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Mouse igm and igg3

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Mouse IgM and IgG3 are laboratory reagents used for immunological research. IgM and IgG3 are immunoglobulin isotypes found in mice. These reagents are used for the detection, quantification, and study of these mouse antibody classes.

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Lab products found in correlation

Np25 bsa, by Biosearch Technologies (2 mentions) 96 well immune plate, by Thermo Fisher Scientific (2 mentions) P nitrophenylphosphate, by Southern Biotech (2 mentions) Ap conjugated anti mouse igm and igg3 antibodies, by Southern Biotech (2 mentions)

2 protocols using mouse igm and igg3

1

NP-BSA Immunization Antibody Assay

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For NP-LPS and NP-Ficoll immunization experiments, 96 well immune-plates (Thermo Fisher Scientific) were coated with NP25-BSA (Biosearch Technologies). Mouse serum samples were incubated for 2h at RT. Standard curves were generated using mouse IgM and IgG3 (Southern Biotech). Bound antibodies were detected by AP-conjugated anti-mouse IgM and IgG3-antibodies (Southern Biotech). Plates were developed with p-nitrophenylphosphate (Southern Biotech) dissolved in substrate buffer.
Milanovic M., Heise N., De Silva N.S., Anderson M.M., Silva K., Carette A., Orelli F., Bhagat G, & Klein U. (2016). Differential requirements for the canonical NF-κB transcription factors c-REL and RELA during the generation and activation of mature B-cells. Immunology and cell biology, 95(3), 261-271.
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2

NP-BSA Immunization Antibody Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
For NP-LPS and NP-Ficoll immunization experiments, 96 well immune-plates (Thermo Fisher Scientific) were coated with NP25-BSA (Biosearch Technologies). Mouse serum samples were incubated for 2h at RT. Standard curves were generated using mouse IgM and IgG3 (Southern Biotech). Bound antibodies were detected by AP-conjugated anti-mouse IgM and IgG3-antibodies (Southern Biotech). Plates were developed with p-nitrophenylphosphate (Southern Biotech) dissolved in substrate buffer.
Milanovic M., Heise N., De Silva N.S., Anderson M.M., Silva K., Carette A., Orelli F., Bhagat G, & Klein U. (2016). Differential requirements for the canonical NF-κB transcription factors c-REL and RELA during the generation and activation of mature B-cells. Immunology and cell biology, 95(3), 261-271.
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