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2 protocols using corilagin

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Phytochemical Characterization and Quantification

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Methanol (HPLC grade and MS grade) was purchased from Fisher Scientific (Waltham, MA, USA). Distilled water was purchased from Watson's Food & Beverage Co., Ltd. (Guangzhou, China). Acetic acid (MS grade) was purchased from Fisher Scientific (Waltham, MA, USA). Reference standards of gallic acid (98%, CAS No: 149-91-7), punicalagin A (63%, CAS No: 65995-63-3), punicalagin B (37%, CAS No: 65995-63-3), methyl gallate (98%, CAS No: 99-24-1), geraniin (98%, CAS No: 2360976-49-0), corilagin (98%, CAS No: 23094-69-1), chebulinic acid (98%, CAS No: 18942-26-2), chebulagic acid (98%, CAS No: 23049-71-5), and ellagic acid (98%, CAS No: 476-66-4) were purchased from Chengdu-PUSH Bio-Technology Co., Ltd. (Chengdu, Sichuan, China).
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2

Evaluation of α-Glucosidase Inhibitors

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The α-Glucosidase (from Saccharomyces cerevisiae) was purchased from Sigma-Aldrich (St. Louis, MO, USA). The p-nitrophenyl-α-D-glucopyranoside (PNPG , ≥99%), acarbose (≥95%), ellagic acid (≥98%), gallic acid (≥98%), protocatehuic acid (≥98%), methyl gallate (≥98%), gallic acid ethyl ester (≥98%), kaempferol-3-Orobinobioside (≥98%) and astragalin (≥98%) reference substances were obtained from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China) . Chromatography grade formic acid (≥98%) and caffeic acid (≥98%) reference substance were purchased from Shanghai Aladdin Reagent Co., Ltd. (Shanghai, China) . Corilagin (≥98%), geraniin (≥98%) and rutin (≥98%) reference substances were obtained from Chengdu Push Biotechnology Co., Ltd. (Chengdu, China) . Isoquercitrin (≥98%) reference substance was purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China) .
Chromatography grade methanol and acetonitrile were obtained from Merck (Darmstadt, Germany). The ultrapure water was purified by Milli-Q 700 ultrapure water system (Merck Millipore, Germany).
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