Human skin biopsies were embedded in OCT, and cryo-sections (10 μm) were attached onto the microscope cover glass (1.2 mm diameter, Fisher Scientific Co., Pittsburgh, PA). These AFM samples were allowed to air dry for at least 24 hours before imaging them to AFM analysis. Images were taken by Dimension Icon AFM system (Bruker-AXS, Santa Barbara, CA, USA) using a silicon AFM probe (PPP-BSI, force constant 0.01–0.5N/m, resonant frequency 12–45kHz, NANOSENSORS™, Switzerland). AFM was conducted at the Electron Microbeam Analysis Laboratory (EMAL), University of Michigan College of Engineering, and analyzed using Nanoscope Analysis software (Nanoscope Analysis v120R1sr3, Bruker-AXS, Santa Barbara, CA, USA).
Microscope cover glass
Manufactured by Thermo Fisher Scientific
Sourced in United States, Switzerland
Microscope cover glass is a transparent thin glass slide used to cover and protect specimens during microscopic examination. It serves as a protective layer to keep the specimen in place and prevent contamination. The cover glass provides a flat, smooth surface to improve optical clarity and focus when viewing samples under a microscope.
Automatically generated - may contain errors
Lab products found in correlation
Axio observer z1, by Zeiss (3 mentions)
Dimension icon afm system, by Bruker (2 mentions)
Nanoscope analysis v120r1sr3, by Bruker (2 mentions)
Genteal, by Novartis (2 mentions)
Lsm 700, by Zeiss (2 mentions)
Fluorescence microscope, by Zeiss (2 mentions)
Alexa 546 labeled goat anti rabbit antibody, by Thermo Fisher Scientific (2 mentions)
Hoechst 33258, by Thermo Fisher Scientific (2 mentions)
Vectashield, by Vector Laboratories (2 mentions)
Tcs sp uv confocal laser scanning microscope, by Leica (2 mentions)
Ppp bsi, by Nanosensors (1 mentions)
Volocity 6, by PerkinElmer (1 mentions)
Formaldehyde, by Thermo Fisher Scientific (1 mentions)
Tcs sp5 confocal microscope, by Leica (1 mentions)
Hoechst 33342, by Merck Group (1 mentions)
Prism software, by GraphPad (1 mentions)
24 well plate, by Corning (1 mentions)
Fluoromount g, by Southern Biotech (1 mentions)
Prolong antifade reagent, by Thermo Fisher Scientific (1 mentions)
Fv1000, by Olympus (1 mentions)
34 protocols using microscope cover glass
1
Atomic Force Microscopy of Human Skin
2
Exfoliation of Perovskite Single Crystals
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Scotch tape (3M) was applied to exfoliate quasi‐2D perovskite single crystal. Briefly, a piece of the sample was transferred onto the adhesive side of the tape. After folding the tape with a blank position facing the single crystal, the two sides of the tape were separated to exfoliate the single crystals. After repeating this process several times, the adhesive side of tape was pressed on a piece of microscope cover glass (24 × 50, Fisherbrand) and the single crystal flakes could be observed by microscopy (Olympus IX71).
3
FRET Analysis of Fos-KDM2A Interaction
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For the FRET assay, HEK293T cells were cultured for 48 hrs after transfection with pECFP-c-Fos and/or pEYFP-KDM2A plasmids, on poly-D-lysine-coated coverslips (Fisher Scientific Microscope Cover Glass, 12-545-80, Hampton, NH, USA) and TPA was added for 2 hours. Cells were fixed by 4% paraformaldehyde and FLIM analysis was performed using a Leica TCS SP8 SMD scanning confocal microscope (Leica Microsystems, Wetzlar, Germany; APO 64 × / NA = 1.4, donor activation wavelength 458 nm). The FRET assay was performed with TCS SP8 confocal software (Leica, Germany).
4
Immunofluorescence Staining and Confocal Microscopy Analysis
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Cells were grown on microscope cover glass (Fisher Scientific, Pittsburgh, PA) in 24-well plate (Corning Inc., Lowell, MA USA). Cells were fixed with 1% formaldehyde (Fisher Scientific) in PBS for 10 min, permeabilized with 0.5% Triton/PBS for 14 min on ice and then incubated for an hour at RT with primary antibodies followed by wash with PBS and visualization with highly cross-absorbed secondary antibodies conjugated with Alexa Fluor 488, 594 or 647 dye (Invitrogen, Carlsbad, CA). Cells were stained with Hoechst 33342 (Sigma) for DNA visualization and mounted on slides (Fisher Scientific) with Fluoromount G (Southern Biotech, Birmingham, AL, USA). Images were analyzed using Leica TCS SP5 confocal microscope.
Quantitative analysis of IF images was performed using Volocity 6.3 software (PerkinElmer). At least 200 cells from 10 randomly picked up fields were analyzed for every clone. Statistical analysis was done by GraphPad Prism software (GraphPad Software, Inc., La Jolla, CA).
Quantitative analysis of IF images was performed using Volocity 6.3 software (PerkinElmer). At least 200 cells from 10 randomly picked up fields were analyzed for every clone. Statistical analysis was done by GraphPad Prism software (GraphPad Software, Inc., La Jolla, CA).
5
Immunofluorescence Assay for HMGB1
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For immunofluorescence assays, cells were cultured in collagen treated-Fisher-Brand Microscope Cover Glass in 12-well plates. For HMGB1, 1.5 × 104 cells were seeded onto coverslips and were grown overnight before treatment. Cells were treated for 48 h with P2Et, Doxorubicin or vehicles and washed with PBS twice. Cells were fixed in 4% formaldehyde for 20 min, washed and permeabilized with 0.1% Triton X-100 for 5 min. This was followed by blocking with 10% FCS PBS for 1 h, and incubation with anti-HMGB1 primary antibody for 30 min at room temperature (RT) and Alexa Fluor 488- conjugated secondary antibody for 30 min. Finally, cells were stained with DAPI (300 nM) for 5 min and coverslips were mounted on slides with Prolong Antifade Reagent (Life Technologies, Woburn, MA, USA). Images of 640 × 640 pixels resolution were acquired with a laser scanning confocal microscope FV1000 (Olympus, Tokyo, Japan) using UPLSAPO 60× 1.35 NA oil immersion objective.
6
Silicone Adhesive for Glass Lamination
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Example 1
Silicone adhesive cured with branched alkenyl siloxane and linear crosslinker. A siloxane solution was formed with non-linear alkenyl organosiloxane ((ViMe2SiO1/2)4(Me2SiO2/2)120(SiO4/2)) having a dynamic viscosity measured at 25° C. of 100-600 centipoise (cp) and linear hydrogenorganosiloxane (((Me3SiO1/2)2(Me2SiO2/2)3-4(HMeSiO2/2)5-6) with Si—H/vinyl ratio of 1.5:1, a Pt catalyst, and diallyl maleate inhibitor, with inhibitor/catalyst ratio of 150:1. This siloxane solution was spin coated on the carrier glass with thickness 10-100 μm, and then cured at 160° C. for 2 minutes. A slim glass (Fisherbrand® Microscope cover glass with dimension of 50 mm×45 mm and 0.13 mm to 0.17 mm thickness) was then laminated on top of the cured silicone on the carrier. The assembly was placed under vacuum at around 10−3 Torr (0.13 pascal) for 1 hour to achieve close adhesion, and then was put in a preheated air circulated oven at 250° C. for 1.5 hours. No outgassing was observed. The slim glass was released from the silicone on the carrier with a 90 degree peel force of 45 g/cm.
7
Silicone Adhesive Curing Mechanism
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Example 2
Silicone adhesive cured with linear alkenyl siloxane and branched crosslinker. A siloxane solution was formed with linear alkenyl organosiloxane ((ViMe2SiO1/2)2(Me2SiO2/2)150) and branched hydrogenorganosiloxane ((HMe2SiO1/2)1.58(SiO4/2) wherein the unit subscripts indicate mole ratios thereof, an oligomer) with Si—H/vinyl ratio of 1.5:1, a Pt catalyst, and diallyl maleate inhibitor with inhibitor/catalyst ratio of 150:1. This siloxane solution was spin coated on the carrier glass with thickness 10-100 μm, and then cured at 160° C. for 2 minutes. A slim glass (Fisherbrand® Microscope cover glass with dimension of 50 mm×45 mm and 0.13 mm to 0.17 mm thickness) was then laminated on top of the cured silicone on the carrier. The assembly was placed under vacuum at around 10−3 Torr (0.13 pascal) for 1 hour to achieve closely adhesion, and then was put in a preheated air circulated oven at 250° C. for 1.5 hours. No outgassing was observed. The slim glass was released from the silicone on the carrier with a 90 degree peel force of 34 g/cm.
8
Immobilizing Quantum Dots on a Coverglass
9
Analyzing DRG Axonal Degeneration
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DRGs axonal degeneration analyses were performed as previously described (Unsain et al., 2014 (link)). Briefly, DRGs grown on filters or plastic culture plates were fixed with 4% paraformaldehyde for 20 min at room temperature and then incubated in blocking solution (TBS-T, 5% skim milk, and 0.3% Triton X-100) for 1 h. DRGs were then incubated overnight with antibodies against β-III tubulin, diluted 1:10,000 in blocking solution. Plates or filters were then incubated with Alexa488-conjugated goat anti-mouse secondary antibodies (Jackson Laboratory, United States) for 2 h at room temperature. The filters were removed from the insert, placed in fluorescent mounting medium (Fluoroshield, Sigma Aldrich, Canada), and mounted on Superfrost Plus slides (Fisher Scientific, Canada) then sealed with microscope cover glass (Fisher Scientific, Canada). Imaging was performed using a Zeiss AxioObserverZ1 inverted epifluorescence microscope at 5× magnification with an automated, motorized stage. Tiled images were stitched automatically with Zen 2 software to produce images of the entire culture plate or filter.
10
Mitochondrial Imaging in HeLa Cells
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HeLa cells cultured in microscope cover glass (Fisher Scientific) were washed with ice-cold phosphate buffered saline (PBS) and fixed with 4% paraformaldehyde. Cells were permeabilized with 0.2% Triton X-100 for 3 min and blocked with 1% bovine serum albumin (BSA) for 1 h at room temperature (RT). Cells were then incubated with the indicated primary antibodies (anti- mitochondria and Flag at 1:200 dilution) for 1 h at RT. Following washing, cells were incubated with proper secondary antibodies (FITC-conjugated goat anti-rabbit or TRITC-conjugated goat anti-mouse IgG) for 45 min at RT, washed and stained with 4, 6-diamidino-2-phenylindole (DAPI) to detect nuclei. Immunofluorescence was performed with a Nikon A1 confocal microscope.
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