Tem ccd camera

Transmission electron microscopy was employed to observe the structure of nanliposomes and chitosan-coated liposome with a negative staining method according to the protocol of Colas et al. (2007) [57 (link)]. Briefly, the samples were diluted 25-folds with distilled water to reduce the concentration of the particles. Same volume of the diluted solution was mixed with an aqueous solution of ammonium molybdate (2%) as a negative staining agent. Staining was followed by a 3 min wait at room temperature, and 5 min on a copper mesh coated with carbon, then samples were examined using a Philips CM20 Transmission Electron Microscope associated with an Olympus TEM CCD camera.

Transmission electron microscopy (TEM) was performed to monitor the microstructure of the nanoparticle samples using negative staining as previously described [31 (link),42 (link)]. The samples were diluted 30-fold with distilled water. An equal volume of 2% ammonium molybdate solution was added to the diluted sample, followed by incubation for 3 min at room temperature. A drop of this solution was placed on a carbon face of EMS CF200-CU type carbon-copper grid (200 mesh). The excess liquid was removed by filter paper, and the grid was left to dry (room temperature, 5 min). Micrographs were taken using a Philips/FEI CM200 transmission electron microscope (ThermoFisher Scientific, Mérignac, France) operating at 200 kV and recorded using an Olympus TEM CCD camera.

The morphology of nanoliposomes was monitored via TEM using a negative staining method as previously described [30 (link)]. In brief, the liposomal formulation was diluted in distilled water (1:10 ratio) and then mixed with a 2% ammonium molybdate solution with a ratio of 1:1. The mixture was reserved at room temperature for 3 min. Then, one drop was placed and dried on a Formvar carbon-coated copper grid (200 mesh, 3 mm diameter HF 36). Then, the morphology of the nanoliposomes was examined using a Philips CM20 TEM equipped with an Olympus TEM CCD camera at 200 kV.

To monitor the morphology of nanoliposomes, TEM was employed using a negative-staining method as described previously [42 (link)]. Briefly, nanoliposomes concentration was reduced by a 30-fold dilution with distilled water. A drop of a solution of 2% ammonium molybdate and the diluted samples was placed for 5 min on a Formvar/carbon supported copper grid (200 mesh, 3 mm diameter HF 36). The mesh was observed using a Philips CM20 operating at 200 kV and micrographs were recorded using an Olympus TEM CCD camera.

To monitor the morphology of the particles contained in MSC-CM, TEM was employed using a negative staining method. Briefly, MSC-CM was diluted 200-fold with distilled water. A drop of diluted sample was mixed with a drop of 2% ammonium molybdate used as a negative staining agent for 3 min at room temperature. The mixture was then placed for 5 min on a Formvar-carbon coated copper grid (200 mesh, 3 mm diameter HF 36). The mesh was examined using a CM20 transmission electron microscope (Philips Healthcare; Suresnes, France) operating at 200 kV and micrographs were recorded with an Olympus TEM CCD camera.

Blank and naringin-loaded nanoliposomes’ structures were observed using transmission electron microscopy (TEM) via a negative staining method, as described by Colas et al. [74 (link)]. Briefly, to reduce the concentration of nanoliposomes, samples were diluted with ultrapure distilled water (25-fold). To stain nanoliposomes, equal volumes of the diluted solution and an aqueous solution of ammonium molybdate (2%)—used as a negative staining agent—were mixed. After the staining procedure, samples were kept at room temperature for 3 min, followed by a 5 min incubation on a copper mesh coated with carbon. Finally, samples were observed using a Philips CM20 TEM coupled with an Olympus TEM CCD camera.

Blank and naringin-loaded nanoliposomes structures were observed using transmission electron microscopy (TEM) via a negative staining method according to Colas et al. protocol [46 (link)]. Briefly, to reduce the concentration of nanoliposomes, samples were diluted with ultrapure distilled water (25-fold). To stain nanoliposomes, equal volumes of the diluted solution and an aqueous solution of ammonium molybdate (2%), used as a negative staining agent, were mixed. After the staining procedure, samples were kept at room temperature for 3 min, followed by a 5 min incubation on a copper mesh coated with carbon. Finally, samples were observed using a CM20 TEM (Philips, Amsterdam, Netherlands) associated with a TEM CCD camera (Olympus, Tokyo, Japan).