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Pezx am04 cmir

Manufactured by GeneCopoeia

PEZX-AM04-cmiR is a lab equipment product. It is a plasmid vector designed for cloning and expressing custom microRNA constructs.

Automatically generated - may contain errors

2 protocols using pezx am04 cmir

1

Lentiviral Transduction of Scp-1 and PASC-1 Cells

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The following plasmids were used for stable lentiviral transduction of Scp-1 and PASC-1 cells: pPACKH1-GAG, pPACKH1-REV, pVSV-G (Systems Biosciences), pLV-[mir-control], pLV-[mir-181a] (Biosettia), pEZX-AM04-cmiR, pEZX-AM04-anti-miR-181a (Genecopeia), pshPER3 #1 TRCN0000018503, pshPER3 #2 TRCN0000018506, pLKO.1-puro Non-Target shRNA (Sigma), psi-LVRU6GP-shPPARG HSH064513, psi-LVRU6GP-shscram CSHCTR001-LVRU6GH (Genecopeia). For 3′UTR luciferase assays pEZX-MT06-PER3 HmiT021640-MT06 (Genecopeia) was used.
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2

Lentiviral Transduction of FTSECs

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The following plasmids were used for stable lentiviral transduction of FTSECs: pPACKH1-GAG, pPACKH1-REV, pVSV-G (Systems Biosciences), pLV-[mir-control], pLV-[mir-181a] (Biosettia), pEZX-AM04-cmiR, pEZX-AM04-anti-miR-181a (Genecopoeia), pshRB1 TRCN0000295842, pLKO.1-puro Non-Target shRNA (Sigma). For 3′UTR luciferase assays pEZX-MT06-RB1 HmiT021640-MT06 and pEZX-MT06-TMEM173 HmiT100627-MT06 (Genecopoeia) was used.
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