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Envisu r2200

Manufactured by Bioptigen
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The Envisu R2200 is a high-resolution, non-invasive imaging system designed for ophthalmic and research applications. It utilizes spectral domain optical coherence tomography (SD-OCT) technology to provide detailed, cross-sectional images of the eye's internal structures.

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Lab products found in correlation

Spectral domain ophthalmic imaging system, by Bioptigen (5 mentions) Spectral domain ophthalmic imaging system sdois, by Bioptigen (4 mentions) Invivovue diver 2, by Bioptigen (3 mentions) Invivovue diver, by Bioptigen (2 mentions) Genteal lubricant eye gel, by Alcon (2 mentions) Tropicol, by Bioptigen (2 mentions) Systane ultra, by Alcon (1 mentions) Genteal tears, by Alcon (1 mentions) Tropicamide, by Alcon (1 mentions) Xylazine, by Akorn (1 mentions) Invivovue clinic software, by Bioptigen (1 mentions) Recugel, by Bausch & Lomb (1 mentions) Aim ras, by Bioptigen (1 mentions) Systane lubricant eye drops, by Alcon (1 mentions) Tropicamide, by Bausch & Lomb (1 mentions) Micron 3 retinal imaging microscope, by Phoenix Pharmaceuticals (1 mentions) Mayer s hematoxylin, by Merck Group (1 mentions) Eosin y, by Thermo Fisher Scientific (1 mentions) Micron 3, by Phoenix Pharmaceuticals (1 mentions) Invivovue program, by Bioptigen (1 mentions)

31 protocols using envisu r2200

1

Quantifying Retinal Layer Thickness in Murine Alzheimer's Models

Cited 2 times
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P301S, P301L mice and age- and strain-matched WT mice were examined by OCT before sacrifice. Briefly, mice were anesthetized via intraperitoneal injection with a combination of ketamine (100 mg/kg) and xylazine (10 mg/kg). Mouse eyes were topically dilated with one drop of tropicamide and phenylephrine. Eyes were imaged using Spectral Domain Ophthalmic Imaging System (Envisu R2200, Bioptigen Inc., NC) as described previously [41 (link)–43 (link)]. Annular scans consisted of 1000 A-scans × 100 B-scans covering a donut-shaped area centered at the optic nerve disc were performed in order to remove variance of optic nerve head measurement. The inner and outer radiuses of the donut-shaped area were 200 and 700 μm from the center of the optic nerve disc, respectively. Then OCT depth thickness report and analysis were generated from Bioptigen’s automated segmentation algorithm developed for the murine eye. The thickness of each retinal layer presented in the report for each eye was an average of 100,000 measurements (A-scans) in the donut-shaped area, which accurately measured retinal thickness and excluded the necessity to register and quantify the same anatomical landmark between different animals. The ganglion cell complex (GCC) includes all three innermost layers: the nerve fiber layer (NFL), the ganglion cell layer (GCL) and the inner plexiform layer (IPL).
Xia F., Ha Y., Shi S., Li Y., Li S., Luisi J., Kayed R., Motamedi M., Liu H, & Zhang W. (2021). Early alterations of neurovascular unit in the retina in mouse models of tauopathy. Acta Neuropathologica Communications, 9, 51.
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2

Retinal Thickness Measurement in Mice

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Retinal thickness was measured in situ in mice under anesthesia using a small animal spectral domain optical coherence tomography (SD-OCT) imaging system (Envisu R2200, Bioptigen) and InVivoVue Diver software (Bioptigen). Measurements were made at 4 compass points 350 μm from center of the optic nerve head and averaged. The total retina spans from the inner limiting membrane (ILM) to the retinal pigment epithelium (RPE). The inner retina spans from the ILM to the inner limit of the outer plexiform layer (OPL). The outer retina spans from the inner limit of the OPL to the RPE.
Abcouwer S.F., Shanmugam S., Muthusamy A., Lin C.M., Kong D., Hager H., Liu X, & Antonetti D.A. (2021). Inflammatory resolution and vascular barrier restoration after retinal ischemia reperfusion injury. Journal of Neuroinflammation, 18, 186.
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3

Retinal Structure Evaluation in rd10 Mice

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Rd10 nontreated and rd10+PTZ mice were anesthetized with ketamine/xylazine as described.16 (link) Retinal structure was evaluated in vivo using a Bioptigen Spectral Domain Ophthalmic Imaging System (SDOIS; Bioptigen Envisu R2200, Morrisville, NC, USA) in mice at ages P60, P90, P120, and P180. The imaging protocol included averaged single B-scan and volume intensity scans with images centered on the optic nerve head. Because of the considerable outer retinal disruption in rd10 retina, it is preferable to use the manual caliper feature to measure retinal layers versus autosegmentation postimaging analysis (a feature of the InVivoVue Diver 2.4 software). We used the manual caliper feature to acquire inner, outer, and total retinal thickness measurements. Inner retina was measured from the superior boundary of inner limiting membrane (ILM) to the lower edge of the inner nuclear layer (INL). Outer retina was measured from the lower edge of the INL to the inferior boundary of the retinal pigment epithelium layer (RPE); if there was separation of photoreceptors from RPE, we obtained outer retinal thickness by adding the RPE layer thickness and the distance from the lower edge of INL to the edge of neuronal retina as described previously.22 (link)
Wang J., Saul A, & Smith S.B. (2019). Activation of Sigma 1 Receptor Extends Survival of Cones and Improves Visual Acuity in a Murine Model of Retinitis Pigmentosa. Investigative Ophthalmology & Visual Science, 60(13), 4397-4407.
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4

Retinal OCT Imaging in Mice

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Mice were anesthetized, and their pupils dilated as described above. Artificial tears (Systane Ultra, Alcon, Fort Worth, TX) were used to maintain corneal hydration and clarity. OCT images were obtained using the Bioptigen Spectral Domain Ophthalmic Imaging System (Bioptigen Envisu R2200, Morrisville, NC). Image acquisition software was provided by the vendor. The thickness of the ONL was measured at a distance of 0.624 mm from the optic nerve head (ONH) using software provided by vendor.
Li Y., Furhang R., Ray A., Duncan T., Soucy J., Mahdi R., Chaitankar V., Gieser L., Poliakov E., Qian H., Liu P., Dong L., Rogozin I.B, & Redmond T.M. (2019). Aberrant RNA splicing is the major pathogenic effect in a knock-in mouse model of the dominantly inherited c.1430A>G human RPE65 mutation. Human mutation, 40(4), 426-443.
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5

Retinal Structural Analysis by SD-OCT

Cited 2 times
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Retinal structure was evaluated using SD-OCT as described (Navneet et al, 2019b (link); Wang et al, 2021 (link)). Briefly, mice were anesthetized by intraperitoneal (i.p.) injection of ketamine 80mg/kg, xylazine 10mg/kg; retinal architecture was assessed using the Bioptigen SD Ophthalmic Imaging System (Bioptigen Envisu R2200, NC). The imaging protocol included averaged single B scan and volume intensity scans with images centered on the optic nerve head. Post-imaging analysis included autosegmentation analysis and manual assessment of retinal layers using InVivoVue™ Diver 2.4 software (Bioptigen). Measurements included the nerve fiber layer (NFL), inner plexiform layer (IPL), inner nuclear layer (INL), outer plexiform layer (OPL), and outer nuclear layer + inner segments (ONL+IS), outer segments (OS) and retinal pigment epithelium (RPE). Each layer thickness was plotted separately; data for a given retinal layer in each group were averaged.
Xiao H., Wang J., Barwick S.R., Yoon Y, & Smith S.B. (2021). Effect of Long-term Chronic Hyperhomocysteinemia on Retinal Structure and Function in the Cystathionine-β-Synthase Mutant Mouse. Experimental eye research, 214, 108894.
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6

Retinal Structure Assessment using OCT and Histology

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Retinal structure was assessed at 7 days on anesthetized mice using OCT (the Bioptigen Spectral Domain Ophthalmic Imaging System, SDOIS; Bioptigen Envisu R2200, NC) as previously described70 (link). Retinal thickness was determined by morphometric analysis on H&E-stained retinal frozen cross sections as previously described19 (link),26 (link),70 (link). Inner retina (INL + IPL + GCL) thickness was measured on H&E images at three different distances from optic nerve head using ImageJ software. Averaged retinal thickness was presented as percentage compared to the contralateral sham eyes.
Fouda A.Y., Xu Z., Shosha E., Lemtalsi T., Chen J., Toque H.A., Tritz R., Cui X., Stansfield B.K., Huo Y., Rodriguez P.C., Smith S.B., Caldwell R.W., Narayanan S.P, & Caldwell R.B. (2018). Arginase 1 promotes retinal neurovascular protection from ischemia through suppression of macrophage inflammatory responses. Cell Death & Disease, 9(10), 1001.
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7

Corneal Thickness and Anterior Chamber Angle Changes Following Alkali Burn

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After mice were anesthetized, one drop of Genteal tears (Alcon Laboratories, Fort Worth, TX) was applied to the eye to maintain corneal hydration. A spectral-domain OCT system (Envisu R2200; Bioptigen, Durham, NC) was used to image the anterior segment of the eye at 0, 3, 7 and 14 days after alkali burn. Full corneal thickness was measured from epithelium to endothelium using ImageJ (Li et al., 2022 (link); Luisi et al., 2021 ). Based on the separation distance between corneal endothelium border and the root of the iris seen in OCT images, we scored changes in anterior chamber angle using the Shaffer grading system (Lange et al., 2009 (link)).
Ouverney C.C, & Fuhrman J.A. (1999). Combined Microautoradiography–16S rRNA Probe Technique for Determination of Radioisotope Uptake by Specific Microbial Cell Types In Situ. Applied and Environmental Microbiology, 65(4), 1746-1752.
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8

Retinal Structure Evaluation in Mice

Cited 4 times
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At P42, retinal structure was evaluated in mice (anesthetized by i.p injection of ketamine 80 mg/kg, xylazine 10 mg/kg) using the Bioptigen Spectral Domain Ophthalmic Imaging System (SDOIS; Bioptigen Envisu R2200, Durham, NC) as described previously (Navneet et al, 2019 (link);Wang et al, 2016b (link); Wang et al, 2019b (link); Xiao et al, 2020 ). We used the manual caliper feature to measure the total retinal thickness, thickness of the inner retina and thickness of the outer retina as described previously (Wang et al, 2019a (link), Mezu-Ndubuisi et al, 2017 (link)). The inner retina measurement extended from the inner limiting membrane (ILM) to the distal edge of the inner nuclear layer (INL). The inner retina included the nerve fiber layer, ganglion cell layer, inner plexiform layer and the INL. The outer retina measurement extended from the distal edge of the INL to the basolateral border of the retinal pigment epithelium layer (RPE) and included the outer plexiform layer, outer nuclear layer (ONL), the inner and outer segments and the RPE. In instances where there was separation (detachment) of PRCs from RPE, which is a common feature of the rd10 retina, we measured the RPE layer thickness and added this value to the outer retinal thickness.
Wang J., Xiao H., Shannon B., Liu Y, & Smith S.B. (2020). Optimal timing for activation of Sigma 1 Receptor in the Pde6brd10/J (rd10) mouse model of retinitis pigmentosa.. Experimental eye research, 202, 108397.
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9

Noninvasive Retinal Neuronal Analysis by SD-OCT

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Spectral-domain (SD)-OCT was used to noninvasively analyze retinal neuronal structure.21 (link) Mice were anesthetized with an intraperitoneal injection of a mixture of ketamine (100 mg/kg) and xylazine (10 mg/kg). Pupils were dilated with tropicamide and phenylephrine, and mice were then positioned on the imaging platform of the Spectral-Domain Ophthalmic Imaging System (Envisu R2200; Bioptigen Inc., Morrisville, NC, USA). An en face scan consisting of 100 B-scans in a rectangular pattern was performed, in which high-resolution B-scans consist of 1000 A-scans with 1.4-μm resolution and 1.4-mm width. Retinal thickness within a donut-shaped area centered at the optic nerve disc was measured in an annular pattern. The inner and outer radiuses of the donut-shaped area were 200 and 700 μm from the center of the optic nerve disc, respectively. Retinal thickness maps and statistics were obtained using Bioptigen's report generator. The analysis included the “mouse report” template, which uses techniques for automated segmentation of the retinal images to generate depth maps and statistical tables of each retinal layer at various depths. The ganglion cell complex (GCC) is defined as the three innermost retinal layers: the nerve fiber layer, the GCL, and the inner plexiform layer.
Ha Y., Liu W., Liu H., Zhu S., Xia F., Gerson J.E., Azhar N.A., Tilton R.G., Motamedi M., Kayed R, & Zhang W. (2018). AAV2-mediated GRP78 Transfer Alleviates Retinal Neuronal Injury by Downregulating ER Stress and Tau Oligomer Formation. Investigative Ophthalmology & Visual Science, 59(11), 4670-4682.
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10

Retinal Integrity Validation via OCT Imaging

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OCT imaging to verify retina integrity after TPM imaging was performed using SD-OCT Envisu R2200 (Bioptigen, Morrisville, NC)
Palczewska G., Dong Z., Golczak M., Hunter J.J., Williams D.R., Alexander N.S, & Palczewski K. (2014). Noninvasive two-photon fluorescence microscopy imaging of mouse retina and RPE through the pupil of the eye. Nature medicine, 20(7), 785-789.
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