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Mouse pro inflammatory 7 plex kit

Manufactured by Mesoscale
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The Mouse Pro-inflammatory 7-Plex kit is a multiplex assay designed to measure the levels of seven pro-inflammatory cytokines in mouse samples. The kit allows for the simultaneous detection and quantification of these target analytes in a single well.

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Lab products found in correlation

Discovery sector 2400 instrument, by Mesoscale (2 mentions) Meso quickplex sq 120 imager, by Mesoscale (2 mentions) Multi array technology, by Mesoscale (1 mentions)

Close spelling variants of this product

Mouse pro-inflammatory 7-Plex tissue culture kit MSD Mouse Pro-inflammatory 7-Plex Ultra-Sensitive Kit Mouse Pro-inflammatory 7-plex

8 protocols using mouse pro inflammatory 7 plex kit

1

Assessing L. monocytogenes Pathogenesis

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Mice were infected with 5 × 104 CFU wild type or 1 × 107 CFU ΔactA mutant Lm (in 200 μl of PBS) via intravenous injection in the lateral tail vein. Liver and spleen were collected at 72 h post infection. Organs were placed in 1 ml of sterile PBS and homogenized. A dilution series was plated on BHI plates, grown overnight at 37°C and individual bacterial colonies were enumerated.
For cytokine analysis, cell culture supernatants and tissue homogenates were centrifuged (12,000 rpm for 12′) and supernatants were collected and stored at −80°C. Cytokines were measured using the mouse 7-plex pro-inflammatory kit from Meso Scale Discovery, according to manufacturer’s instructions.
Czuczman M.A., Fattouh R., van Rijn J., Canadien V., Osborne S., Muise A.M., Kuchroo V.K., Higgins D.E, & Brumell J.H. (2014). Listeria monocytogenes exploits efferocytosis to promote cell-to-cell spread. Nature, 509(7499), 230-234.
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2

Assessing L. monocytogenes Pathogenesis

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Mice were infected with 5 × 104 CFU wild type or 1 × 107 CFU ΔactA mutant Lm (in 200 μl of PBS) via intravenous injection in the lateral tail vein. Liver and spleen were collected at 72 h post infection. Organs were placed in 1 ml of sterile PBS and homogenized. A dilution series was plated on BHI plates, grown overnight at 37°C and individual bacterial colonies were enumerated.
For cytokine analysis, cell culture supernatants and tissue homogenates were centrifuged (12,000 rpm for 12′) and supernatants were collected and stored at −80°C. Cytokines were measured using the mouse 7-plex pro-inflammatory kit from Meso Scale Discovery, according to manufacturer’s instructions.
Czuczman M.A., Fattouh R., van Rijn J., Canadien V., Osborne S., Muise A.M., Kuchroo V.K., Higgins D.E, & Brumell J.H. (2014). Listeria monocytogenes exploits efferocytosis to promote cell-to-cell spread. Nature, 509(7499), 230-234.
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3

Multiplexed Immunoassay for Cytokines

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Serum samples were subjected to multiplexed immunoassays by the Emory Multiplexed Immunoassay Core (EMIC), using a mouse proinflammatory 7-PLEX kit (Meso-Scale, Rockville, MD) and the Discovery Sector 2400 instrument (Meso-Scale). Samples were analyzed for cytokines IFNγ, TNFα, chemokine (C-X-C motif) ligand 1 (CXCL1, mKC), IL1, IL6, IL10, and IL12.
Nyagode B.A., Jahangardi R., Merrell M.D., Tansey M.G, & Morgan E.T. (2014). Selective effects of a therapeutic protein targeting tumor necrosis factor-alpha on cytochrome P450 regulation during infectious colitis: implications for disease-dependent drug–drug interactions. Pharmacology Research & Perspectives, 2(1), e00027.
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4

Cytokine Profiling via Multiplexed Immunoassay

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Serum samples were subjected to multiplexed immunoassays by the Emory Multiplexed Immunoassay Core (EMIC), using a mouse pro-inflammatory 7-PLEX kit (Meso-Scale, Rockville, MD) and the Discovery Sector 2400 instrument (Meso-Scale). Samples were analyzed for cytokines IFNγ, TNFα, chemokine (C-X-C motif) ligand 1 (CXCL1, mKC), IL1, IL6, IL10 and IL12.
Nyagode B.A., Jahangardi R., Merrell M.D., Tansey M.G, & Morgan E.T. (2014). Selective effects of a therapeutic protein targeting tumor necrosis factor-alpha on cytochrome P450 regulation during infectious colitis: implications for disease-dependent drug–drug interactions. Pharmacology Research & Perspectives, 2(1), e00027.
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5

Serum Proinflammatory Profiling in Mice

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Following the last hypersensitivity study, mice were anesthetized and serum samples taken. The serum was analyzed by Leidos Clinical Services Program using a Meso Scale Discovery mouse proinflammatory 7-plex kit.
Zhao Z., Fan H., Higgins T., Qi J., Haines D., Trivett A., Oppenheim J.J., Wei H., Li J., Lin H, & Howard O.M. (2014). Fufang Kushen injection inhibits sarcoma growth and tumor-induced hyperalgesia via TRPV1 signaling pathways. Cancer letters, 355(2), 232-241.
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6

Colon Tissue Cytokine Profiling

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Colon tissue was homogenized, and cytokine protein levels were measured using a Mouse Pro-inflammatory 7-Plex kit (Meso Scale Diagnostics, Rockville, MD) read on a MESO QuickPlex SQ 120 imager (Meso Scale), as described in detail in the supplemental methods.
Gao R.Y., Shearn C.T., Orlicky D.J., Battista K.D., Alexeev E.E., Cartwright I.M., Lanis J.M., Kostelecky R.E., Ju C., Colgan S.P, & Fennimore B.P. (2020). Bile Acids Modulate Colonic MAdCAM-1 Expression in a Murine Model of Combined Cholestasis and Colitis. Mucosal immunology, 14(2), 479-490.
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7

Colon Tissue Cytokine Profiling

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Colon tissue was homogenized, and cytokine protein levels were measured using a Mouse Pro-inflammatory 7-Plex kit (Meso Scale Diagnostics, Rockville, MD) read on a MESO QuickPlex SQ 120 imager (Meso Scale), as described in detail in the supplemental methods.
Gao R.Y., Shearn C.T., Orlicky D.J., Battista K.D., Alexeev E.E., Cartwright I.M., Lanis J.M., Kostelecky R.E., Ju C., Colgan S.P, & Fennimore B.P. (2020). Bile Acids Modulate Colonic MAdCAM-1 Expression in a Murine Model of Combined Cholestasis and Colitis. Mucosal immunology, 14(2), 479-490.
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8

Quantitative Cytokine Profiling via Electrochemiluminescence

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Multi-Array technology by Meso Scale Diagnostics (Rockville, MD, USA) allows quantitative assessment of expression levels of proteins using electrochemiluminescence. To evaluate concentration of proinflammatory cytokines, a Mouse Proinflammatory 7-Plex kit (Meso Scale Diagnostics, Rockville, MD, USA) was used. Assay was performed in accordance to manufacturer’s manual. The plate was read using a Sector S 600 analyzer (Meso Scale Diagnostics, Rockville, MD, USA).
Papinska A.M., Soto M., Meeks C.J, & Rodgers K.E. (2016). Long-term administration of angiotensin (1-7) prevents heart and lung dysfunction in a mouse model of type 2 diabetes (db/db) by reducing oxidative stress, inflammation and pathological remodeling. Pharmacological research, 107, 372-380.
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