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Discovery v12

Manufactured by Zeiss
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Sourced in Germany

The Discovery V12 is a versatile lab equipment product from Zeiss. It is designed for high-resolution imaging and analysis of a wide range of samples. The core function of the Discovery V12 is to provide advanced optical performance and flexibility for various research and industrial applications.

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Lab products found in correlation

Axiocam, by Zeiss (6 mentions) Axiocam mrc5, by Zeiss (3 mentions) Axiocam hrc, by Zeiss (2 mentions) Axiocam mrc camera, by Zeiss (2 mentions) Axiocam erc 5s, by Nikon (2 mentions) Jenalab compound microscope, by Zeiss (2 mentions) Stemi sv11, by Zeiss (2 mentions) Axiocam mrc, by Zeiss (2 mentions) Axioskop 2 plus, by Zeiss (2 mentions) Lsm 710, by Zeiss (2 mentions) Coolpix 995, by Nikon (2 mentions) Axioskop, by Zeiss (1 mentions) Focus software, by Helicon (1 mentions) Illustrator cs6, by Adobe (1 mentions) Zen core v2, by Zeiss (1 mentions) Sta7300, by Hitachi (1 mentions) Murashige and skoog basal salt micronutrient solution, by Merck Group (1 mentions) Axiocam 506 color camera, by Zeiss (1 mentions) Axio imager m1, by Zeiss (1 mentions) Ab6789, by Abcam (1 mentions)

59 protocols using discovery v12

1

Scoring Nematode Mouth-Form Phenotypes

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The mouth-form phenotype was scored using the following characters to discriminate between Eu and St individuals, respectively, (i) the presence versus absence of a subventral tooth, (ii) a claw-like versus flint-like or triangular dorsal tooth, and (iii) a wide versus narrow stoma (mouth). Characters (i) and (ii) were discrete, non-overlapping, and sufficient to distinguish the two forms. Apparent intermediates between the two forms were rare (<0.1%) and were not included in counts. Phenotypes could be scored using Zeiss Discovery V.12 stereomicroscopes and were supplemented where necessary with differential interference contrast (DIC) microscopy on a Zeiss Axioskop.
Serobyan V., Xiao H., Namdeo S., Rödelsperger C., Sieriebriennikov B., Witte H., Röseler W, & Sommer R.J. (2016). Chromatin remodelling and antisense-mediated up-regulation of the developmental switch gene eud-1 control predatory feeding plasticity. Nature Communications, 7, 12337.
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2

Examination of Insect Genitalia Specimens

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Our terminology follows McKittrick 1964 [34] , Grandcolas 1996 [35] , Anisyutkin 2010 [36] and Anisyutkin 2013 [37] (link). The genital segments of the examined specimens were macerated in 10% KOH and observed in glycerin with a Zeiss Discovery V12 stereomicroscope. Wings were floated in hot water until fully spread, embedded in neutral balsam, then mounted on slides and covered with coverslips. Drawings were made using a Zeiss Discovery V12 stereomicroscope fitted with a Canon PowerShot G1X digital camera and drawn using Adobe Illustrator CS6. All images of specimens were photographed using a Canon 60D plus a Canon EF 100 mm f/2.8L IS USM Macro lens combined with Helicon Focus software. All specimens studied were pinned in a natural posture and deposited in the medical vector collections of the Zhongshan Entry-Exit Inspection and Quarantine Bureau (ZSCIQ). The specimens we collected in China fully match other published morphological descriptions of H. concinna individuals from other geographic locations [6] , [38] , [39] .
Yue Q., Wu K., Qiu D., Hu J., Liu D., Wei X., Chen J, & Cook C.E. (2014). A Formal Re-Description of the Cockroach Hebardina concinna Anchored on DNA Barcodes Confirms Wing Polymorphism and Identifies Morphological Characters for Field Identification. PLoS ONE, 9(9), e106789.
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3

Swelling Characterization of DLV Foams

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To assess swelling, the DLV foams were imaged in both the lyophilized and rehydrated states with a Zeiss Discovery V12 stereomicroscope (Carl Zeiss Microscopy) (n = 4, N = 2). The dimensions of the foams were determined from the stereomicroscope images using ImageJ analysis software to determine the dry (VD) and hydrated (VH) volumes of the cylindrical foams. The swelling ratio (SR) was then calculated according to the following formula:

Russo V., Omidi E., Samani A., Hamilton A, & Flynn L.E. (2015). Porous, Ventricular Extracellular Matrix-Derived Foams as a Platform for Cardiac Cell Culture. BioResearch Open Access, 4(1), 374-388.
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4

Zebrafish Enhancer Screening Assay

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All experiments involving zebrafish (Danio rerio, AB strain) were approved by the Vanderbilt Institutional Animal Care and Use Committee, Nashville, TN. Microinjection was done as described Ni et al 201236 (link). Briefly, a mixture of individual enhancer-containing vector DNA (25μg/ml) and transposase RNA (25μg/ml) was injected into zebrafish zygotes (1 nl/zygote). The injected embryos were cultured in 0.3x Danieau’s solution at 28.5°C. After 24 hours, the embryos were examined for EGFP expression under a fluorescent dissecting microscope (Zeiss Discovery V12) to determine the stereotypic expression pattern conferred by the enhancer. The total number of embryos injected with the construct and the number of embryos with the stereotypical EGFP pattern were determined to calculate the frequency of the pattern. Embryos were dechorionated and imaged using a Zeiss AxioCam HRc digital camera. At a minimum, ~150–200 embryos were injected per reporter construct and assays were repeated 2–3 times per construct to confirm reproducibility.
Lin C.Y., Erkek S., Tong Y., Yin L., Federation A.J., Zapatka M., Haldipur P., Kawauchi D., Risch T., Warnatz H.J., Worst B.C., Ju B., Orr B.A., Zeid R., Polaski D.R., Segura-Wang M., Waszak S.M., Jones D.T., Kool M., Hovestadt V., Buchhalter I., Sieber L., Johann P., Chavez L., Gröschel S., Ryzhova M., Korshunov A., Chen W., Chizhikov V.V., Millen K.J., Amstislavskiy V., Lehrach H., Yaspo M.L., Eils R., Lichter P., Korbel J.O., Pfister S.M., Bradner J.E, & Northcott P.A. (2016). Active medulloblastoma enhancers reveal subgroup-specific cellular origins. Nature, 530(7588), 57-62.
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5

Fluorescent Lineage Tracing in Embryos

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Fluorescent lineage tracer (FLDx) in embryos was observed using a Zeiss Discovery V12 microscope with fluorescent illumination and 470nm GFP filter set. Photos were taken with a Zeiss AxioCam Mrc5 CCD camera assembled on the microscope, using Axiovision 4.8 software.
Fish M.B., Nakayama T., Fisher M., Hirsch N., Cox A., Reeder R., Carruthers S., Hall A., Stemple D.L, & Grainger R.M. (2014). Xenopus mutant reveals necessity of rax for specifying the eye field which otherwise forms tissue with telencephalic and diencephalic character. Developmental biology, 395(2), 317-330.
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6

Swelling Kinetics of Hollow Filaments

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Dry hollow filaments were cut into 750 µm thin slices with a cutting tool that consisted of several razor blades fixed at a defined distance on a metal rod. The slices were placed under a stereomicroscope (Zeiss, Discovery.V12). Swelling of the material was monitored at 37-fold magnification after addition of PBS. The maximum degree of swelling was reached after 15 min. The outer and inner diameter of the tubes and wall thickness were calculated in microscopic images using ZEN core v2.7 software. Three sections per filament were analyzed. Three independently fabricated hollow filament batches were measured. From each batch, three filaments were used.
Prade I., Schröpfer M., Seidel C., Krumbiegel C., Hille T., Sonntag F., Behrens S., Schmieder F., Voigt B, & Meyer M. (2022). Human endothelial cells form an endothelium in freestanding collagen hollow filaments fabricated by direct extrusion printing. Biomaterials and Biosystems, 8, 100067.
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7

Collagen Filament Shrinkage Analysis

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Shrinkage of the collagen filaments during cultivation was analyzed with pieces of 10 mm length. Individual cell-laden and cell-free collagen tubes were transferred into 6-wells and fully covered with EGM-2 media. Medium was changed every 2–3 days. After 7 and 14 days of incubation at 37 °C in a CO2-incubator, collagen tubes were analyzed with a stereomicroscope (Zeiss, Discovery.V12) and Zeiss software (ZEN core v2.7). Three independently fabricated collagen filament batches were tested. From each batch three filaments were analyzed in triplicates.
Prade I., Schröpfer M., Seidel C., Krumbiegel C., Hille T., Sonntag F., Behrens S., Schmieder F., Voigt B, & Meyer M. (2022). Human endothelial cells form an endothelium in freestanding collagen hollow filaments fabricated by direct extrusion printing. Biomaterials and Biosystems, 8, 100067.
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8

Characterization of Powder Morphology and Slurry Rheology

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Powder morphology was characterized according to the Brunauer‐Emmett‐Teller theory (BET porosimetry) and by Hg intrusion measurements by means of Micromeritics ASAP 2020 and MicroActive AutoPore V9600 instrument, respectively. N2 physisorption was not observed and only the inter‐particle intrusion was detected, thus excluding the intra‐particle one. Thus, powder porosity was considered negligible and not considered in the assessment of the slurry formulation.
Powder particle size distribution was investigated through laser granulometry, using a CILAS 1180 instrument (Compagnie Industrielle des Lasers, Orléans, France).
Slurry rheological properties were assessed by a rotational rheometer set‐up with a disc‐plate configuration (DSR 200 device by Rheometrics) using a parallel disc geometry (upper plated diameter equal to 40 mm, 0.3 mm gap); viscosity was investigated in the shear rate range between 10−1 and 103 seconds−1.
Differential Thermal Analysis‐Thermogravimetry (DTA‐TG) was performed using a STA7300 instrument (Hitachi). Measurements were carried out in air from room temperature up to 900°C, with a heating rate of 5°C·min−1.
Washcoat layer homogeneity and morphology were evaluated by optical microscopy, using a SteREO Discovery V12 instrument equipped with an Axiocam ERc 5 seconds camera by Zeiss.
Balzarotti R., Bisaccia A., Tripi M.C., Ambrosetti M., Groppi G, & Tronconi E. (2020). Production and characterization of copper periodic open cellular structures made by 3D printing‐replica technique. Journal of Advanced Manufacturing and Processing, 2(4), e10068.
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9

Quantifying Magnetic Particle Interactions

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Videos were recorded at 300 fps using the Basler acA800-510uc camera mounted on a stereomicroscope (Zeiss Discovery V12). They were analyzed using a custom script in MATLAB. DHM images were recorded and analyzed on Lyncée Tec reflection DHM R2200. DHM is also used to measure the distance between the top of the magnet and the air-water interface. The error bars of every point in the distance plots of pairwise interactions were estimated from 300 video frames for each point.
Wang W., Giltinan J., Zakharchenko S, & Sitti M. (2017). Dynamic and programmable self-assembly of micro-rafts at the air-water interface. Science Advances, 3(5), e1602522.
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10

Glucose-Supplemented Plant Growth

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Plants were grown on medium containing 0.15 or 0.3 mM KNO3, 1.5 mM CaCl2, 0.75 mM MgSO4, 0.625 mM KH2PO4 and 200 mM glucose, supplemented with Murashige and Skoog basal salt micronutrient solution (Sigma-Aldrich). ½ MS with 200 mM glucose was used as mock control. Images were taken 5 days after germination. Images were taken using a Zeiss Discovery.V12 stereomicroscope equipped with a Zeiss AxioCam HRc.
Durr J., Papareddy R., Nakajima K, & Gutierrez-Marcos J. (2018). Highly efficient heritable targeted deletions of gene clusters and non-coding regulatory regions in Arabidopsis using CRISPR/Cas9. Scientific Reports, 8, 4443.
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