Ta 2513

Catalase (Cat. No. CA2517), and total antioxidant capacity (TAC, Cat. No. TA 2513) were assessed spectrophotometrically in colonic tissue homogenate using commercial kits supplied by Bio-diagnostic (Giza, Egypt). Myeloperoxidase (MPO) activity (Cat. No. MAK068-1 KT) was assessed using a kit supplied by Sigma-Aldrich (MO, United States), where MPO catalyzes the formation of hypochlorous acid, which reacts with taurine to form taurine chloroamine. A colorless product, DTNB, is formed when taurine chloroamine reacts with the chromophore.

Total antioxidant capacity (TAC) in serum was determined by colorimetric method using a trade available kit (Cat. no# TA 2513) obtained from Bio-Diagnostics Company, Egypt, following previously outlined procedures (Koracevic et al., 2001 (link)). The anti-oxidative capacity in rat serum is measured by the reaction of antioxidants in the sample with a known amount of exogenously supplied hydrogen peroxide (H₂O₂), resulting in removing a specific amount of H₂O₂. The remaining H₂O₂ is estimated colorimetrically by an enzymatic reaction, which involves the conversion of 3, 5, dichloro–2–hydroxyl benzensulphonate to a colored product. The level of serum malondialdehyde (MDA), a major lipid peroxidation product, is measured colorimetrically using a commercially available kit (Cat. no# MD2529) obtained from Bio-Diagnostics Company, Egypt, following previously outlined instructions (Ohkawa et al., 1979 (link)). This assay is based on the reaction of thiobarbituric acid (TBA) with MDA in an acidic medium at a temperature of 95°C for 30 min to form a thiobarbituric acid reactive product with a pink color, with its absorbance measured at 534 nm. Serum TAC is measured in mM/L, while serum MDA is measured in nmol/ml.

The lung tissues were homogenized in 1–2 ml of 5 mM cold potassium phosphate buffer (pH 7.4), centrifuged at 4000 rpm for 15 min at 4 °C and the supernatant was removed and stored at − 80 °C for assay of total antioxidant capacity (TAC) (Cat. No. TA 25 13, Biodiagnostics) and malondialdehyde (MDA) (Cat. No. MD 25 29, Biodiagnostics).