RA-FLSs (1.5 × 105 / well) treated as indicated were collected and stained with Annexin V-FITC/propidium iodide (PI) Apoptosis Detection kit (Elabscience, China). Cell death analysis was performed on the BD FACS Caliber flow cytometer.
Annexin 5 fitc propidium iodide apoptosis detection kit
Manufactured by Elabscience
Sourced in China
The Annexin V-FITC/propidium iodide (PI) Apoptosis Detection kit is a tool used to detect and quantify apoptosis, a type of programmed cell death, in cell samples. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, and propidium iodide, a fluorescent dye that binds to DNA, to identify cells at different stages of apoptosis.
Automatically generated - may contain errors
Lab products found in correlation
Facs caliber flow cytometer, by BD (1 mentions)
Arginine glycine aspartic acid, by Merck Group (1 mentions)
Hoechst 33342, by Thermo Fisher Scientific (1 mentions)
Tert butyl alcohol tbuoh, by Thermo Fisher Scientific (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Rmpi 1640, by Thermo Fisher Scientific (1 mentions)
P formaldehyde, by Merck Group (1 mentions)
Facsvia flow cytometer, by BD (1 mentions)
4 protocols using annexin 5 fitc propidium iodide apoptosis detection kit
1
RA-FLSs Apoptosis Assessment
2
Cytotoxicity Evaluation of Zinc-based Nanomaterial
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Imidazole-2-carboxaldehyde (2-ICA; 99% purity), zinc nitrate hexahydrate (Zn(NO3)2∙6H2O; 99% purity), polyvinylpyrrolidone (PVP; average mol. wt. = 40000), p-formaldehyde (95% purity), phosphate buffered saline (PBS), fluorescein (95% purity), arginine-glycine-aspartic acid (RGD), cisplatin (Cis; 99% purity), hydrochloric acids, and dimethyl sulfoxide (DMSO; 99.5% purity) were obtained from Sigma Aldrich. Methanol (99.9% purity) and tert-butyl alcohol (t-BuOH; 99% purity) were purchased from Fisher Scientific. RMPI 1640, fetal bovine serum (FBS), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and trypsin were acquired from Gibco (Grand Island, USA). Hoechst33342 was procured from Invitrogen. Annexin V-FITC/propidium iodide (PI) apoptosis detection kit was received from Elabscience. A Milli-Q gradient water system was used to produce ultra-pure water. Normal human lung fibroblast cells (MRC-5 cells) and adenocarcinoma human alveolar epithelial cells (A549 cells) were obtained from the American Type Culture Collection (ATCC). All materials detailed herein were used without further purification.
3
Apoptosis Quantification via Annexin V-FITC/PI
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An Annexin V-FITC/Propidium iodide (PI) apoptosis detection kit (Elabscience Biotechnology, Wuhan, China) was used to detect cell apoptosis. After treating cells with OSW-1 for 24 h, the cells were digested to generate single-cell suspensions, which were stained according to the kit instructions. The experiment was replicated three times for each measurement.
4
Melatonin Modulates Apoptosis and PD-L1 in NSCLC
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To detect cell apoptosis, NSCLC cells (5 × 105 cells/well) were seeded into 6-well plates and treated with melatonin for 24 h. Cells were trypsinized followed by incubation with an Annexin V-FITC/propidium iodide (PI) Apoptosis detection kit (Elabscience) according to the manufacturer’s instruction. To measure PD-L1 protein levels, cells were treated with melatonin (2.5 mM) in the presence or absence of IFNγ (50 ng/mL) for 24 h. Cells were then trypsinized followed by incubation with a PE-conjugated anti-PD-L1 antibody (Abcam) and analyzed with a BD FACSVia flow cytometer (BD Biosciences).
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