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B6 cg gt

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B6.Cg-Gt is a laboratory mouse strain. It is a congenic strain that carries a transgene.

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Lab products found in correlation

B6 cg tg, by Jackson ImmunoResearch (2 mentions) Hze j, by Jackson ImmunoResearch (2 mentions) B6d2f1 j, by Jackson ImmunoResearch (1 mentions) B6 cg, by Jackson ImmunoResearch (1 mentions) Cd 1 mice, by Charles River Laboratories (1 mentions) C57bl 6j mice, by Jackson ImmunoResearch (1 mentions) Mouse thymus cell antigen 1 thy 1 promoter, by Jackson ImmunoResearch (1 mentions) B6 cg 7630403g23riktg th cre 1tmd j, by Jackson ImmunoResearch (1 mentions) 26sortm9 cag tdtomato hze j, by Jackson ImmunoResearch (1 mentions) B6 cg tg prrx1 cre 1cjt j, by Jackson ImmunoResearch (1 mentions) 26sortm9, by Jackson ImmunoResearch (1 mentions) C57bl 6j, by Jackson ImmunoResearch (1 mentions)

6 protocols using b6 cg gt

1

Conditional Knockout Mice for Adipose Tissue

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All animal experiments were carried out conforming to a protocol approved by the University of Pennsylvania Institutional Animal Care and Use Committee (IACUC #804855). The following mice were purchased from Jackson Laboratory: B6.Cg-Tg(Prrx1-cre)1Cjt/J (Prx1Cre), B6.Cg-Tg(Prrx1-cre/ERT2,-EGFP)1Smkm/J (Prx1CreER), B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J (R26TdTomato), Tg(Adipoq-cre)1Evdr (Adipoq-Cre), Tg(Col1a2-cre/ERT,-ALPP)7Cpd/J (Col1a2-CreER). Floxed Ikkβ mice (Ikkβf/f) were obtained from M. Karin (University of California, San Diego). Ikkb-deletion by Cre-mediated recombination was validated as in fig. S10. Mice were housed in regular corn bedding and were euthanized at 1-day, 4-week, and 16–20-week-old time points. In another set of experiments, animals were housed in Cellu-nest™ (Shepherd Specialty Papers) soft bedding from birth to the 4th week euthanasia time point.
Ko K.I., Merlet J.J., DerGarabedian B.P., Zhen H., Horiuchi Y., Hedberg M.L., Hu E., Nguyen A.T., Prouty S., Alawi F., Walsh M.C., Choi Y., Millar S.E., Cliff A., Romero J., Garvin M.R., Seykora J.T., Jacobson D, & Graves D.T. (2022). NF-kB perturbation reveals unique immunomodulatory functions in Prx1+ fibroblasts that promote development of atopic dermatitis. Science translational medicine, 14(630), eabj0324.
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2

Transgenic Mouse Lines for Lineage Tracing

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B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J (007914), B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J (007676), B6D2F1/J (100006), B6.Cg-Pdgfrbtm1.1(cre/ERT2)Csln/J (030201), B6.Cg-Tg(Tek-cre)1Ywa/J (008863), B6.129P2(C)-Cx3cr1tm2.1(cre/ERT2)Jung/J (020940), and B6.129S4-Gt(ROSA)26Sortm2(FLP*)Sor/J (012930) were obtained from Jackson Laboratories. Hoxa13Cre-WPRE and Hoxa13Cre mice generated in this study were described below. For timed matings, embryonic day 0.5 (E0.5) was identified by the time a vaginal plug was discovered. The pregnant dams at the indicated times were sacrificed and the embryo and placenta were dissected out from the uterus. All experiments were performed at least twice using different litters and used littermate controls on a mixed background unless otherwise indicated. These mice were maintained in a specific pathogen-free environment under a 14/10 hour light/dark cycle and all animal experiments described were performed in accordance and approval of the University of Pennsylvania Institutional Animal Care and Use Committee.
Chen X., Tang A.T., Tober J., Yang J., Leu N.A., Sterling S., Chen M., Yang Y., Mericko-Ishizuka P., Speck N.A, & Kahn M.L. (2022). Mouse placenta fetal macrophages arise from endothelial cells outside the placenta. Developmental cell, 57(23), 2652-2660.e3.
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3

Murine Lineage Tracing Protocols

Cited 1 time
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All mouse protocols were approved by the Animal Care Committee at the University of Toronto, which operates in accordance with the Canadian Council on Animal Care. Mice were kept on a 12-h light dark/light cycle. Food was available ad libitum. Water was supplied ad libitum except during EdU delivery (see below). The number of mice used in each experiment is indicated in the figure captions. Adult mice used in this study were 8–24 weeks old and included: CD1 mice (022, Charles River), C57/BL6J mice (000,664, Jackson Laboratories), B6.Cg-Gt(Rosa26)SorTm14(CAG−tdTomato)Hze mice [35 (link)] (007,914, Jackson Laboratories) and Msx1-CreERT2 mice [36 (link)]. The Msx1-CreERT2 mouse line was a gift from Dr. Michel Cayouette and Dr. Benoît Robert and the CreERT2 construct originated from the IGBMC via Pierre Chambon. Msx1-CreERT2 is a transgenic line where the CreERT2 fusion protein is expressed in place of the endogenous Msx1 protein, as it has been knocked in, in frame, in the first exon of Msx1. Msx1-CreERT2 mice were crossed with B6.Cg-Gt(Rosa26)SorTm14(CAG−tdTomato)Hze mice to generate Msx1-CreERT2;B6.Cg-Gt (Rosa26)SorTm14(CAG−tdTomato)Hze mice, which were used for lineage tracing experiments. Each mouse used was genotyped by PCR amplification. For full list of genotyping primers see Additional file 1.
Grisé K.N., Coles B.L., Bautista N.X, & van der Kooy D. (2021). Activation of adult mammalian retinal stem cells in vivo via antagonism of BMP and sFRP2. Stem Cell Research & Therapy, 12, 560.
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4

Generating Dopaminergic Neuron Reporter Mice

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We bred the following two mouse strains from The Jackson Laboratory to generate a tdTomato reporter mice for dopaminergic neurons: Cre-dependent tdTomato reporter mice [B6.Cg-Gt(Cooper et al.)26Sortm9(CAG-tdTomato)Hze/J, stock #007909], and tyrosine hydroxylase (TH) promotor-driven Cre expression (B6.Cg-7630403G23RikTg(Th-cre)1Tmd/J, stock #008601). In order to focally stimulate SNr GABAergic neurons in ex vivo brain slices, we used transgenic mice expressing ChR2 fused to YFP under the control of mouse thymus cell antigen 1 (Thy 1) promoter (stock #007612, The Jackson Laboratory), which specifically express ChR2 in SNr GABA neurons, but not in SN dopaminergic neurons. All mice used for the study were between four and eight weeks old. To detect transgene and floxed alleles, we used a genotyping service (Transnetyx).
Singh M.R., Vigh J, & Amberg G.C. (2021). Angiotensin-II Modulates GABAergic Neurotransmission in the Mouse Substantia Nigra. eNeuro, 8(2), ENEURO.0090-21.2021.
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5

Genetic Labeling of Inhibitory Neurons in Mice

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All experiments were performed with approval of protocols from Stanford University Institutional Animal Care and Use Committee. B6J.129S6(FVB)-Slc32a1tm2(cre)Lowl/MwarJ (VGat-Cre; catalog #028862; https://www.jax.org/strain/028862), B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J (Ai14; catalog #007914; https://www.jax.org/strain/007914), and B6.129P2-Pitx3tm1Mli/Mmjax (Pitx3-GFP) (catalog #41479; https://www.jax.org/strain/028554) mice were obtained from The Jackson Laboratory and bred at Stanford University. Males and females were both used in experiments. Although we were not sufficiently powered to determine sex differences, we identify the number of samples from males and females in each experiment (Table 1). Mice were housed in a 12 h light/dark (LD) cycle and fed ad libitum.
Manning C.E., Fritz M, & Kauer J.A. (2022). Function of Excitatory Periaqueductal Gray Synapses in the Ventral Tegmental Area following Inflammatory Injury. eNeuro, 9(6), ENEURO.0324-22.2022.
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6

Diabetic Fracture Healing in Mice

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All animal experiments were initiated on 8- to 10-week-old male and female mice conforming to a protocol approved by the University of Pennsylvania Institutional Animal Care and Use Committee. The following mice were purchased from The Jackson Laboratory: C57BL/6J, B6.Cg-Tg(Prrx1-cre)1Cjt/J (Prx1Cre), B6.Cg-Tg(Prrx1-cre/ERT2,-EGFP)1Smkm/J (Prx1CreER), B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J (R26TdTomato), and B6.129P2-Gt(ROSA)26Sortm1(DTA)Lky/J (R26DTA). B6;SJL-Tg(Col2α1-cre)1Bhr/J mice (Col2α1Cre) were obtained from Dr. Patrick O’Connor (Rutgers University, Newark, NJ), and floxed Ikkβ mice (Ikkβf/f) from Dr. Michael Karin (University of California San Diego, La Jolla, CA). Low-dose streptozotocin injection was performed intraperitoneally for T1D induction (5 consecutive days, 50 mg/kg) (Cayman Chemical) as previously described (22 (link)). Animals were considered diabetic when blood glucose was >220 mg/dL 1 week after last injection and remained diabetic for 4–6 weeks prior to fracture surgery.
Ko K.I., Syverson A.L., Kralik R.M., Choi J., DerGarabedian B.P., Chen C, & Graves D.T. (2019). Diabetes-Induced NF-κB Dysregulation in Skeletal Stem Cells Prevents Resolution of Inflammation. Diabetes, 68(11), 2095-2106.
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