Dextromethorphan hydrobromide, chloroquine, denatonium benzoate, quinine hydrochloride, phenylthiocarbamide (PTC), 6-n-propylthiouracil (PROP), sodium thiocyanate, salicin, nicotine, yohimbine, colchicine, thiamine, caffeine, chloramphenicol and picrotoxinin were purchased from Sigma. Fluo-4 NW calcium assay kit was purchased from Invitrogen. hPASMCs and hASMCs were either purchased from ATCC or were a kind gift from Dr. Andrew Halayko, Dept. of Physiology, University of Manitoba. Cell culture media and hPASMC growth kit were purchased from Cedarlane, Canada. Nucleofection kit for smooth muscle cell transfection was purchased from Lonza. Polyclonal antibodies specific for T2R1, T2R38 and normal IgG specific rabbit antisera were obtained from Abcam (Cambridge, MA, USA) and Santa Cruz Biotechnology (Dallas, TX, USA) respectively [6] (link), [7] (link). Phospho-MLC-Ser19 monoclonal antibody and MLC polyclonal antibody were purchased from Cell Signaling technology (Danvers, MA, USA). DyNazyme hot start was purchased from Thermo Fisher Scientific (Toronto, ON, Canada). The shRNA specific for T2R1 was purchased from Qiagen (Toronto, ON, Canada).
6 n propylthiouracil
Manufactured by Merck Group
Sourced in Australia
6-n-Propylthiouracil is a chemical compound used in laboratory research. It functions as a thyroid peroxidase inhibitor, which plays a role in the synthesis of thyroid hormones. The core function of this product is to inhibit thyroid peroxidase activity, without further interpretation of its intended use.
Automatically generated - may contain errors
Lab products found in correlation
Yohimbine, by Merck Group (1 mentions)
Quinine hydrochloride, by Merck Group (1 mentions)
Salicin, by Merck Group (1 mentions)
Dextromethorphan hydrobromide, by Merck Group (1 mentions)
Phenylthiocarbamide, by Merck Group (1 mentions)
Sodium thiocyanate, by Merck Group (1 mentions)
Chloramphenicol, by Merck Group (1 mentions)
Chloroquine, by Merck Group (1 mentions)
Fluo 4 nw calcium assay kit, by Thermo Fisher Scientific (1 mentions)
Thiamine, by Merck Group (1 mentions)
Colchicine, by Merck Group (1 mentions)
Hasmc, by American Type Culture Collection (1 mentions)
Picrotoxinin, by Merck Group (1 mentions)
Caffeine, by Merck Group (1 mentions)
Nicotine, by Merck Group (1 mentions)
Water purification system, by Merck Group (1 mentions)
Copper 2 chloride dehydrate, by Merck Group (1 mentions)
Neocuprine, by Merck Group (1 mentions)
Ammonium acetate, by Merck Group (1 mentions)
Gallic acid, by Merck Group (1 mentions)
4 protocols using 6 n propylthiouracil
1
Investigating Taste Receptor Signaling in Smooth Muscle Cells
2
Antioxidant Activity Assay Protocol
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Chemicals used in this study included Ethanol, (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 6-n-propylthiouracil (PROP), Ammonium Acetate, Copper (II) Chloride Dehydrate, Folin-Ciocalteu Reagent, Gallic Acid, Neocuprine, and Sodium Carbonate and were purchased from Sigma-Aldrich (Castle Hill, Sydney, Australia). The DI water was prepared using the Millipore water purification system (Millipore Australia, North Ryde, NSW, Australia).
3
Quantification of Propylthiouracil Using Spectrophotometry
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PROP preparation and quantification were performed as described.17 (link) Briefly, 50 mg propylthiouracil (PTU) tablets were purchased from T.O. Chemical manufacturer. No. 1 Whatman filters (3 cm diameter, Sigma-Aldrich) were cut in half and saturated with a 50-mmol/L PROP solution. PROP was eluted from randomly selected saturated disks by incubating each disk in 20 mL methanol overnight at room temperature. The amount of PROP in the impregnated filter paper disks was quantified using spectrophotometry. Pure PROP (6-n-propylthiouracil, Sigma-Aldrich) was dissolved in methanol and used as a standard. The PROP standard was prepared at 6 concentrations (0–0.01 mg/mL). The absorbance of PROP in the methanol solution was measured at a 275-nm wavelength. A calibration curve was constructed and the extracted PROP concentration was determined. We ascertained that the mean amount of PROP per disk was 0.412 ± 0.017 mg, and the percentage coefficient of variation across the disks (n = 3) was 4.2%.
4
Measuring PROP Bitterness Perception
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PROP testing strips were prepared using 8.5 g of 6-n-propylthiouracil (Sigma Catalog #P3755) in 500 mls of distilled water. This mixture was stirred on a hotplate until dissolved. Whatman’s No. 1 filter paper was then briefly and completely submerged into the solution, removed and allowed to dry. Dry PROP papers were cut into 1 cm2 strips and stored at room temperature in a sterile petri dish sealed with Parafilm. For each experiment, subjects were first asked to rinse their mouths with distilled water. The investigator then removed a single PROP testing strip from the petri dish using sterile forceps and placed it on the subject’s palm. The subject placed the PROP strip onto the anterior tip of their tongue for 15 seconds and rated the intensity of the taste using a ratio scale, the general Labeled Magnitude Scale, which was developed for sensory testing including PROP bitterness perception and comparison. This scale ranges from 0 (not detectable) to 100 (strongest imaginable sensation of any kind) [77 (link), 78 ]. Participant responses were recorded by hand and then included in each subject’s coded file.
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