Thioflavin t tht ultrapure grade

Fluorescent dye thioflavin T (ThT) “UltraPure Grade” (AnaSpec, Fremont, CA, USA), isopropyl-beta-D-1-thiogalactopyranoside (IPTG; Fluka, Switzerland), buffer components, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) from Sigma (USA) were used without further purification. For amyloid preparation, Aβ40 and Aβ42 peptides from GL Biochem, Shanghai, China, and 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) from Sigma-Aldrich, St. Louis, MO, USA, were used. DMEM (glucose 4.5 g/L), fetal bovine serum (FBS), 0.25% trypsin-EDTA were purchased from Gibco (Thermo Fisher Scientific, Waltham, MA, USA). Culture flasks and 96-well plates (flat bottom) were purchased from Corning (New York, NY, USA). Dulbecco’s Modified Eagle Medium (DMEM), fetal bovine serum (FBS), penicillin, and streptomycin were purchased from Gibco BRL (Life Technologies, Paisley, Scotland). The human cervical cancer cell line (HeLa) was obtained from the cell bank (The Center of Collective Usage “Collection of Vertebrate Cell Cultures”, Institute of Cytology, RAS)). 3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), rhodamine 123, and propidium iodide were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Guanidine hydrochloride (GdnHCl), lysozyme, buffer components, trypsin from bovine pancreas, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma (St. Louis, MO, USA). Thioflavin T (ThT) “UltraPure Grade” was supplied by AnaSpec (Fremont, CA, USA), and 8-anilino-1-naphthalene sulfonate (ANS) was from Serva (Heidelberg, Germany). Further purification of chemicals has not been performed. Reagents for cell cultivation, including DMEM medium (glucose 4.5 g/L), fetal bovine serum (FBS), and 0.25% Trypsin-EDTA were acquired from Gibco (Thermo Fisher Scientific, Waltham, MA, USA). Culture flasks and 96-well plates (flat bottom) were from Corning (New York, NY, USA).

Fluorescent dye thioflavin T (ThT) “UltraPure Grade” from AnaSpec (United States ), Aβ42 peptide from GL Biochem (China), 1,1,1,3,3,3-Hexafluoro-2-propanol (HFIP), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), guanidine hydrochloride (GdnHCl), trypsin from bovine pancreas, and buffer components from Sigma (United States ), isopropyl-beta-D-1-thiogalactopyranoside (IPTG) from Fluka (Switzerland) were used without additional purification. Reagents for cell cultivation, including DMEM medium (glucose 4.5 g/L), fetal bovine serum (FBS), and 0.25% Trypsin-EDTA were acquired from Gibco (Thermo Fisher Scientific, United States ). Culture flasks and 96-well plates (flat bottom) were purchased from Corning (United States ).