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Vamp3

Manufactured by Proteintech
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VAMP3 is a recombinant protein that is part of the VAMP (Vesicle-Associated Membrane Protein) family. VAMP3 is a core component of the SNARE complex, which is involved in the fusion of vesicles with the target membrane during intracellular trafficking.

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Lab products found in correlation

Tsg101, by Proteintech (3 mentions) Rhodamine phalloidin, by Thermo Fisher Scientific (2 mentions) Draq5, by Thermo Fisher Scientific (2 mentions) Alexa fluor 647 phalloidin, by Thermo Fisher Scientific (2 mentions) Antibodies to β actin and ha tag, by Cell Signaling Technology (2 mentions) Antibodies against double stranded dna, by Abcam (2 mentions) Histone h3 tri methyl k9, by Abcam (2 mentions) Emerin, by Proteintech (2 mentions) Apex1, by Proteintech (2 mentions) Annexin a1, by Proteintech (2 mentions) Mouse monoclonal antibody against human cd63 h5c6, by Developmental Studies Hybridoma Bank (2 mentions) Alexa fluor plus 488, by Thermo Fisher Scientific (2 mentions) Alexa fluor plus 555, by Thermo Fisher Scientific (2 mentions) Alexa fluor plus 680, by Thermo Fisher Scientific (2 mentions) Alexa fluor plus 800, by Thermo Fisher Scientific (2 mentions) Nuc green stain, by Thermo Fisher Scientific (2 mentions) α tubulin, by Merck Group (2 mentions) Integrin β1, by Merck Group (2 mentions) High capacity magnetic sepharose beads, by Merck Group (2 mentions) Histone h3, by Proteintech (2 mentions)

3 protocols using vamp3

1

Antibody Reagents for Extracellular Vesicle Analysis

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Antibodies to β-actin and HA tag were purchased from Cell Signaling Technologies. Antibodies against double stranded DNA and Histone-H3 (tri-methyl K9) were purchased from Abcam. β1-integrin and α-tubulin antibodies were purchased from MilliporeSigma. CD63 antibody used for immunoblotting was obtained from System Biosciences International. VAMP3, ARF6, Emerin, APEX1, Annexin-A1, Flotillin, Alix, CD81, TSG-101, SRY, and Histone H-3 antibodies, along with rabbit polyclonal antibody against cGAS, were purchased from Proteintech. Mouse monoclonal antibody against human CD63 (H5C6) used for immunofluorescence was purchased from the Developmental Studies Hybridoma Bank. Antibody against GFP was purchased from Life Technologies. Fluorophore conjugated secondary antibodies (donkey anti-mouse Alexa Fluor Plus 488, donkey anti-rabbit Alexa Fluor Plus 555, donkey anti-mouse Alexa Fluor Plus 680, and donkey anti-rabbit Alexa Fluor Plus 800), rhodamine phalloidin, Alexa Fluor 647-phalloidin, To-Pro-3 iodide, Nuc Green stain, and DRAQ-5 were purchased from Life Technologies. High capacity magnetic sepharose beads were purchased from MilliporeSigma. Unless noted, all other chemicals were purchased from V.W.R.
Clancy J.W., Sheehan C.S., Boomgarden A.C, & D’Souza-Schorey C. (2022). Recruitment of DNA to tumor-derived microvesicles. Cell reports, 38(9), 110443.
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2

Protein Extraction and Western Blot Analysis

Cited 1 time
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Protein extraction and WB assays were performed as previously described [28 (link)]. Primary antibodies targeting the following proteins were used in our WB analysis: Lamp2 (Proteintech, Wuhan, China, 27823-1-AP, 1:2000), EEA1 (Proteintech, 28347-1-AP, 1:2000), LC3B (Proteintech, 18725-1-AP, 1:2000), CD63 (Abcam, Burlingame, CA, USA, ab134045, 1:2000), GAPDH (Proteintech, 60004-1-Ig, 1:2000), NOS3 (Proteintech, 27120-1-AP, 1:2000), VAMP3 (Proteintech, 10702-1-AP, 1:2000), TSG101 (Proteintech, 28283-1-AP, 1:2000), Calnexin (Proteintech, 10427-2-AP, 1:2000).
Hu Y., Zang W., Feng Y., Mao Q., Chen J., Zhu Y, & Xue W. (2024). mir-605-3p prevents liver premetastatic niche formation by inhibiting angiogenesis via decreasing exosomal nos3 release in gastric cancer. Cancer Cell International, 24, 184.
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3

Antibody Reagents for Extracellular Vesicle Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies to β-actin and HA tag were purchased from Cell Signaling Technologies. Antibodies against double stranded DNA and Histone-H3 (tri-methyl K9) were purchased from Abcam. β1-integrin and α-tubulin antibodies were purchased from MilliporeSigma. CD63 antibody used for immunoblotting was obtained from System Biosciences International. VAMP3, ARF6, Emerin, APEX1, Annexin-A1, Flotillin, Alix, CD81, TSG-101, SRY, and Histone H-3 antibodies, along with rabbit polyclonal antibody against cGAS, were purchased from Proteintech. Mouse monoclonal antibody against human CD63 (H5C6) used for immunofluorescence was purchased from the Developmental Studies Hybridoma Bank. Antibody against GFP was purchased from Life Technologies. Fluorophore conjugated secondary antibodies (donkey anti-mouse Alexa Fluor Plus 488, donkey anti-rabbit Alexa Fluor Plus 555, donkey anti-mouse Alexa Fluor Plus 680, and donkey anti-rabbit Alexa Fluor Plus 800), rhodamine phalloidin, Alexa Fluor 647-phalloidin, To-Pro-3 iodide, Nuc Green stain, and DRAQ-5 were purchased from Life Technologies. High capacity magnetic sepharose beads were purchased from MilliporeSigma. Unless noted, all other chemicals were purchased from V.W.R.
LaMantia M., Miura T., Tachikawa H., Kaplan H.A., Lennarz W.J, & Mizunaga T. (1991). Glycosylation site binding protein and protein disulfide isomerase are identical and essential for cell viability in yeast.. Proceedings of the National Academy of Sciences of the United States of America, 88(10), 4453-4457.
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