Plain glass microscope slides

Manufactured by Thermo Fisher Scientific

Sourced in United Kingdom

Plain glass microscope slides are a fundamental piece of laboratory equipment used for a variety of scientific applications. They provide a flat, transparent surface to mount and observe samples under a microscope. These slides are made of high-quality glass, offering a clear and durable platform for sample preparation and analysis.

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Lab products found in correlation

Sylgard 184, by Dow (2 mentions) Noa81, by Thorlabs (2 mentions) Methanol meoh, by Thermo Fisher Scientific (2 mentions) Difco gelatin, by Thermo Fisher Scientific (2 mentions) 2 5 dihydroxybenzoic acid dhb, by Thermo Fisher Scientific (2 mentions) Human hemoglobin, by Merck Group (1 mentions) Immobilon p pvdf membrane, by Merck Group (1 mentions) Plasma cleaner pdc 32g, by Harrick (1 mentions) Neutral buffered formalin, by Merck Group (1 mentions) Sodium phosphate monobasic nah2po4, by Thermo Fisher Scientific (1 mentions) Filtration system, by Merck Group (1 mentions) Filtration system, by Thermo Fisher Scientific (1 mentions)

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6 protocols using plain glass microscope slides

Hydrogel Microwell Array Fabrication

Cited 2 times

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Polyethylene glycol diacrylate (PEGDA) hydrogel posts were covalently attached to glass substrates using methacrylate-terminated silane monolayer formation using standard methods.66 ,71 (link) The plain glass microscope slides (Thermo Fisher) were cut into desired dimensions using a diamond scribe (Ted Pella) and running and nipping pliers (Fletcher–Terry) and stored under vacuum until usage. Polydimethylsiloxane (PDMS, Sylgard® 184, Dow Corning) molds85 (link) were made using standard soft-lithography protocols by mixing elastomer base and curing agent in a 10 : 1 ratio and cured on a SU-8 (MicroChem) master that was prepared using standard photolithography protocols. Molds were designed to create 1 × 1 cm arrays of wells with diameters of 300 μm and 30 μm and depths of 35 μm and 38.6 μm, respectively (Fig. S1†). The arrays contained indexing marks in place of some wells. The individual 1 × 1 cm PDMS molds were cut using a scalpel and had 1.5 mm inlets punched out (Biopsy Punch, Miltex). Norland Optical Adhesive 81 (NOA81, Thorlabs) well arrays were formed on the acrylated glass slides by filling the PDMS molds using degas-driven flow,86 (link) UV curing the NOA81, and removing the PDMS molds (see ESI,† Fig. S2).

Tentori A.M., Nagarajan M.B., Kim J.J., Zhang W.C., Slack F.J, & Doyle P.S. (2018). Quantitative and multiplex microRNA assays from unprocessed cells in isolated nanoliter well arrays †Electronic supplementary information (ESI) available. See DOI: 10.1039/c8lc00498f. Lab on a Chip, 18(16), 2410-2424.

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Protein Deposition on PVDF and Glass Slides

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Lyophilized horse cardiac muscle myoglobin and human hemoglobin were purchased from Sigma Aldrich (Gillingham, UK) and used without further purification. One hundred μM solutions of each were prepared in HPLC grade water, (J. T. Baker, Deventer, The Netherlands). Five μL aliquots were spotted onto Immobilon P PVDF membranes (Millipore, Watford, UK) and plain glass microscope slides (Thermo Scientific, Loughborough, UK) at marked positions. Glass microscope slides were first prepared by rinsing with HPLC grade water and air drying. Following deposition of protein solutions, samples were left to air dry for 4 h prior to analysis.

Martin N.J., Griffiths R.L., Edwards R.L, & Cooper H.J. (2015). Native Liquid Extraction Surface Analysis Mass Spectrometry: Analysis of Noncovalent Protein Complexes Directly from Dried Substrates. Journal of the American Society for Mass Spectrometry, 26(8), 1320-1327.

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Covalent Attachment of PEGDA Hydrogels on Glass

Cited 2 times

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Polyethylene glycol diacrylate (PEGDA) hydrogel posts were covalently attached to glass substrates using methacrylate-terminated silane monolayer formation using standard methods.^{66 ,71 (link)} The plain glass microscope slides (Thermo Fisher) were cut into desired dimensions using a diamond scribe (Ted Pella) and running and nipping pliers (Fletcher-Terry) and stored under vacuum until usage. Polydimethylsiloxane (PDMS, Sylgard® 184, Dow Corning) molds^{85 (link)} were made using standard soft-lithography protocols by mixing elastomer base and curing agent in a 10:1 ratio and cured on a SU-8 (MicroChem) master that was prepared using standard photolithography protocols. Molds were designed to create 1 × 1 cm arrays of wells with diameters of 300 μm and 30 μm and depths of 35 μm and 38.6 μΜ, respectively (Fig. S1^†). The arrays contained indexing marks in place of some wells. The individual 1 × 1 cm PDMS molds were cut using a scalpel and had 1.5 mm inlets punched out (Biopsy Punch, Miltex). Norland Optical Adhesive 81 (NOA81, Thorlabs) well arrays were formed on the acrylated glass slides by filling the PDMS molds using degas-driven flow,^{86 (link)} UV curing the NOA81, and removing the PDMS molds (see ESI,f Fig. S2).

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Preparation of LK7β Peptide Thin Films

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The LK₇β peptide was obtained as a lyophilized powder (GL Biochem Ltd.). The powder was dissolved in H₂O, H₂¹⁸O (Sigma Aldrich; 97 atom % ¹⁸O), D₂O (Cambridge Isotope Laboratories, Inc., Andover, MA; 99.9 atom % D), D₂¹⁸O (Sigma Aldrich; 99 atom % D, 95 atom % ¹⁸O), or a mixture of H₂O and D₂O at 1 mM concentration in 100 μL aliquots. The aliquots were frozen in liquid nitrogen and then stored at −80 °C until time of use. Plain glass microscope slides (Thermo Scientific, Portsmouth, NH) were plasma-cleaned (Harrick Plasma, Ithaca, NY; Plasma Cleaner PDC-32G), and 100 μL of defrosted LK₇β solution was pipetted onto the slide. The slides were then dried in a sealed container with desiccant and purged with dry nitrogen to avoid replacement of water isotopes by atmospheric water. The LK₇β peptide formed a hydrated thin film on the glass slides for the SFG experiments.(55 –57 )

Perets E.A, & Yan E.C. (2019). Chiral Water Superstructures Around Anti-parallel β-sheets Observed by Chiral Vibrational Sum Frequency Generation Spectroscopy. The journal of physical chemistry letters, 10(12), 3395-3401.

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MALDI-TOF Mass Spectrometry Protocol

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All water used in this study was doubly distilled on a Millipore filtration system (Burlington, MA) or Fisher HPLC grade. Difco gelatin, plain glass microscope slides, all crab saline components (see below in Tissue Collection section), and methanol (MeOH) were obtained from Fisher Scientific (Pittsburgh, PA). Formic acid (FA) was purchased from Fluka (Mexico City, Mexico), and the 2,5-dihydroxybenzoic acid (DHB) was obtained from Acros Organics (Morristown, New Jersey). 10% neutral buffered formalin (94:4:1:0.5:0.5 (v/v/v/w/w) H₂O:CH₂O:MeOH:NaH₂PO₄:Na₂HPO₄) was purchased from Sigma-Aldrich (Saint Louis, MO). Sodium phosphate, monobasic (NaH₂PO₄) and sodium phosphate, dibasic (anhydrous) (Na₂HPO₄) were purchased from Fisher Scientific (Pittsburgh, PA).

Vu N.Q., Buchberger A.R., Johnson J, & Li L. (2021). Complementary Neuropeptide Detection in Crustacean Brain by Mass Spectrometry Imaging Using Formalin and Alternative Aqueous Tissue Washes. Analytical and bioanalytical chemistry, 413(10), 2665-2673.

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Reagent Preparation for Biomolecular Analysis

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All water (H₂O) used in this study was doubly distilled on a Millipore filtration system (Burlington, MA) or Fisher HPLC grade. Difco™ gelatin, plain glass microscope slides, all crab saline components (see below), and methanol (MeOH) were obtained from Fisher Scientific (Pittsburgh, PA). EtOH utilized in this study was from Pharmco-Aaper (Chicago, IL). Formic acid (FA) was purchased from Fluka (Mexico City, Mexico), and the 2, 5-dihydroxybenzoic acid (DHB) was obtained from Acros Organics (Morris, New Jersey).

Buchberger A.R., Vu N.Q., Johnson J., DeLaney K, & Li L. (2020). A Simple and Effective Sample Preparation Strategy for MALDI-MS Imaging of Neuropeptide Changes in the Crustacean Brain due to Hypoxia and Hypercapnia Stress. Journal of the American Society for Mass Spectrometry, 31(5), 1058-1065.

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