Model 704 glucose analyzer

Plasma glucose was measured using a Hitachi model 704 glucose analyzer (Roche Hitachi, Indianapolis, IN) with intra-assay and interassay coefficients of variation of 1.2 and 1.6%, respectively. Plasma insulin was measured by radioimmunoassay using an anti-insulin serum raised in guinea pigs, ¹²⁵I-labeled insulin (Linco, St. Louis, MO), and a double antibody method to separate bound from free tracer. Assays of fasting plasma lipid profiles were performed in a laboratory that is NHLBI/Centers for Disease Control and Prevention standardized, with the LDL cholesterol concentration calculated using the Friedewald equation. C-reactive protein (CRP) was measured using a high-sensitivity ELISA. HbA_1c was measured in red blood cells using high-performance liquid chromatography methods.

Measurements of total cholesterol, HDL-C, and triglycerides (TGs) were performed as previously described (17 (link)). LDL-C was calculated using the Friedewald equation. Glucose was measured using a Hitachi model 704 glucose analyzer (Roche Hitachi, Indianapolis, IN) and insulin was measured by radioimmunoassay (RIA) with an anti-insulin serum raised in guinea pigs, ¹²⁵I-labeled insulin (Linco, St Louis, MO) and a double antibody method to separate bound from free tracer.