Rat anti-mouse bcl-2 and Bim antibody were purchased from Santa Cruz Biotechnology, Santa Cruz, CA. Recombinant HMGB1 was purchased from R&D System, Minneapolis, MI. Rat anti-mouse p53, phosphorylated p53, bcl-2 and Bax antibody were purchased from Cell Signaling Technology, Beverly, MA. MAPK family antibody sampler kit and phospho-MAPK family antibody sampler kit were purchased from Cell Signaling Technology, Beverly, MA. Annexin V- fluorescein isothiocyante (FITC) was purchased from BD, San Diego, CA. The p38 MAPK inhibitor (SB203580) was purchased from Selleck Chemicals, Houston, TX. Enzyme-linked immunosorbent assay (ELISA) kits of IL-12, IL-2, IL-4, interferon (IFN)-γ, and TNF-α were purchased from Biosource, Worcester, MA. Nuclear extract and nuclear factor of activated T cell (NF-AT) assay kits were purchased from Active Motif, Carlsbad, CA.
Phosphorylated p53
Manufactured by Cell Signaling Technology
Sourced in United States
Phosphorylated p53 is a protein that has been chemically modified by the addition of a phosphate group. This modification can affect the protein's function and activity. The phosphorylated form of p53 is important for cellular processes such as DNA repair, cell cycle regulation, and apoptosis.
Automatically generated - may contain errors
Lab products found in correlation
Exoquick tc exosome isolation reagent, by System Biosciences (3 mentions)
Mapk family antibody sampler kit, by Cell Signaling Technology (1 mentions)
Bax antibody, by Cell Signaling Technology (1 mentions)
Recombinant hmgb1, by R&D Systems (1 mentions)
P38 mapk inhibitor sb203580, by Selleck Chemicals (1 mentions)
Tnf α, by Thermo Fisher Scientific (1 mentions)
Phospho mapk family antibody sampler kit, by Cell Signaling Technology (1 mentions)
Bcl 2, by Cell Signaling Technology (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Casp3, by Abcam (1 mentions)
Seramir exosome rna isolation kit, by System Biosciences (1 mentions)
Penicillin streptomycin solution p s, by Thermo Fisher Scientific (1 mentions)
Casp9, by Abcam (1 mentions)
α mem media, by Thermo Fisher Scientific (1 mentions)
Rpmi medium, by Thermo Fisher Scientific (1 mentions)
Trypsin edta solution, by Thermo Fisher Scientific (1 mentions)
β actin, by Abcam (1 mentions)
Bcl 2, by Abcam (1 mentions)
Bcl2 associated x (bax), by Abcam (1 mentions)
Phosphorylated p70s6k, by Cell Signaling Technology (1 mentions)
5 protocols using phosphorylated p53
1
Apoptosis and Cytokine Signaling Assays
2
Isolation and Analysis of Exosomes
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ExoQuick-TC exosome-isolation reagent (EXOTC50A-1), SeraMir exosome RNA-isolation kit (RA808A-1), exosome-specific marker anti-αCD63 antibody (EXOABCD63A-1), and florescence exosome-preparation reagent (EXDC300A-1) were from System Biosciences (SBI, Palo Alto, CA, USA). Penicillin-streptomycin (PS) solution, trypsin-EDTA solution, RPMI medium, and α-MEM media were obtained from Life Technologies GIBCO (Grand Island, NY, USA). Fetal bovine serum (FBS) was from Sigma-Aldrich (St. Louis, MO, USA). Antibodies used for immunoblotting were against phosphorylated p53 (Cell Signaling Technology, Beverly, MA, USA), BAX, BCL2, CASP9, CASP3, β-actin (Abcam, Cambridge, MA), and cytochrome c (ENZO Diagnostics Inc., Farmingdale, NY, USA).
3
Western Blot Analysis of Dkk-1, p53, and p-p53
4
Immunoblotting Analysis of Cell Signaling
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Cell lysate was subjected to sodium dodecyl sulfate‐polyacrylamide gel electrophoresis. The protein was transferred to a nitrocellulose membrane and hybridized with antibody against AMPKα (catalog number: #2532), phosphorylated AMPKα at Thr 172 (#2535), 4E‐BP1 (#9452), phosphorylated 4E‐BP1 at Thr 37/46 (#9459), p70S6K (#9202), phosphorylated p70S6K at Thr389 (#9205), phosphorylated p53 at Ser 15 (#9284), AKT (#9272), phosphorylated AKT at Ser 473 (#9271), ACC at Ser 79 (#3661), actin (#4970) (Cell Signaling, Danvers, MA, USA), phosphorylated H2AX at Ser 139 (#613401) (BioLegend, San Diego, CA, USA), p53 (Ab‐6, clone DO‐1) and tubulin‐α (clone DM1A, Thermo Fisher Scientific, Fremont, CA, USA) followed by an appropriate second antibody. The membranes were developed with the ECL system (GE Healthcare, Buckinghamshire, UK). actin and tubulin‐α were used as a loading control. DMSO, a solvent of nutlin‐3a, was used as a control.
5
Immunohistochemical Analysis of Dental Tissues
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The specimens were embedded in wax using conventional methods. Sections (4 μm thickness) of the specimens were boiled in 10 mM citrate buffer (pH 6.0) for 20 min and cooled at room temperature for 20 min. The specimens were incubated with primary antibodies against HIF-1α (Abcam, CAM, UK; dilution 1:200), phosphorylated p53 (Cell signaling; dilution 1:100), cleaved Caspase-3 (Cell signaling, MA, USA; dilution 1:100), PCNA (Abcam; dilution 1:200), Glut-1(Abcam; dilution 1:200), Glut-2 (Novus biologicals, CR, USA; dilution 1:200), MMP20 (Abcam; dilution 1:200), Amelx (Santa Cruz Biotechnology, TX, USA; dilution 1:200), Dmp1 (LSBio, WA, USA; dilution 1:200), Dspp (Santa Cruz Biotechnology; dilution 1:200) at 4°C overnight. The specimens were incubated with goat anti-rabbit Alexa Fluor 488 (Thermo Fisher Scientific, MA, USA; dilution 1:200), goat anti-mouse Alexa Fluor 555 (Thermo Fisher Scientific; dilution 1:200) antibodies. The sections were counterstained with DAPI (Molecular Probes, OR, USA, dilution 1:1,000) and examined using a confocal laser microscope (DMi8; Leica, Wetzlar, Germany).
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