Patients’ blood ketone levels and blood glucose levels (BGL) were measured prior to 18F-FDG injection. Ketone levels were measured using the FreeStyle Optium Neo Ketone monitoring system (Abbott Diabetes Care, UK) which measures beta-hydroxybutyrate (BHB). Typically, BHB accounts for 78% of circulating ketone bodies, while acetoacetate and acetone account for 20% and 2% of circulating ketone bodies, respectively (Laffel 1999 (link)); hence, BHB is a suitable marker of an individual’s ketotic state. Patients with serum BHB levels ≥ 0.5 mmol/L were deemed to be in ketosis, and those with levels < 0.5 mmol/L were deemed not ketotic (Laffel 1999 (link)). BGL was measured using the FreeStyle Optium Neo H monitoring system (Abbott Diabetes Care, UK).
Freestyle optium neo h
Manufactured by Abbott
Sourced in United Kingdom, France
The FreeStyle Optium Neo H is a handheld blood glucose monitoring system designed for the management of diabetes. It provides accurate and reliable blood glucose readings to assist individuals in monitoring their condition.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using freestyle optium neo h
1
Measuring Ketosis and Glucose in PET Imaging
2
Biomarkers in fed rodent model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Haematocrit, glycaemia, and lactatemia were measured during tissue collection using a Mini Compur M1100 microspin, FreeStyle Optium Neo H glucometer (Abbott Diabetes Care), and Accutrend® Plus lactate analyser (Roche Diagnostics GmbH), respectively. Sera were prepared from blood samples removed from the tail vein under isoflurane (3%) anaesthesia between 10 am and 12 am in the fed state, and used for the measurement of corticosterone (MyBiosource) and β‐hydroxybutyrate (APExBIO) concentrations.
3
Blood Sample Collection and Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A capillary whole blood sample was collected at the finger tip of each fasting participant using microtainer contact-activated lancets (Becton Dickinson, USA), and was immediately used for the measurement of blood glucose concentration (mg/dl) with the Freestyle Optium Neo H point-of-care device (Abbott Diabetes Care, France).
Blood samples were also collected by venipuncture in four 5 ml serum separation tubes, and in two 5 ml EDTA tubes (Becton Dickinson, USA). Serum separation tubes and EDTA tubes were maintained at 4°C until centrifugation for 15 min at 2,450 and 800 g, respectively. Part of the serum and plasma samples were used by the clinical laboratory of ILM to measure several hematological and biochemical parameters as described inTable 2 . The remaining samples of serum, plasma and red blood cell pellets were aliquoted in several tubes and stored at −20°C until processing as detailed in Table 3 .
Blood samples were also collected by venipuncture in four 5 ml serum separation tubes, and in two 5 ml EDTA tubes (Becton Dickinson, USA). Serum separation tubes and EDTA tubes were maintained at 4°C until centrifugation for 15 min at 2,450 and 800 g, respectively. Part of the serum and plasma samples were used by the clinical laboratory of ILM to measure several hematological and biochemical parameters as described in
4
CSF Collection and Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CSF was obtained from the South Australian Cerebrospinal Fluid Biobank (Flinders University) from patients with normal pressure hydrocephalus and idiopathic intracranial hypertension. CSF collection was approved by the human ethics committee of the South Australian Local Health Network, and all patients provided informed consent. CSF was collected via lumbar puncture into 10-ml tubes and immediately stored at 4°C. After centrifuging at 2000g for 10 min at 4°C, cell-free CSF was stored at −80°C. Before use, CSF samples were filter-sterilized and combined in batches to reduce variability. All samples were clear and colorless. The osmolality (measured using a Fiske Micro-Osmometer, Model 210), protein concentration, and glucose concentration of each sample are reported in table S2. The glucose concentration of CSF samples was measured using a FreeStyle Optium Neo H blood glucose meter (Abbott).
5
Fasting effects on rat metabolism
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Male Wistar-Han rats (Charles River Laboratories) weighing 300–350 g were used in this study. They were kept on a 12:12-hour dark:light cycle with ad libitum access to food and water. All experiments complied with the ethical rules of the European directive (2010/63/EU) for experimentation with laboratory animals and were approved by the ethics review committee of Paris Descartes University (approval no. 18–090 #20,159). For fasting experiments, food was withdrawn from the cage on day 0 at 8.30 am and the bedding was changed to eliminate pellet residues and limit coprophagy. Rats were then sacrificed 24 h, 48 h, or 72 h later at 8.30 am. Control animals were fed ad libitum and all animals had free access to water. Rats were weighed on day 0 and on the day of sacrifice. Glycemia and ketonemia were measured just before sacrifice on a drop of tail-tip blood using a Freestyle Optium Neo H (Abbott) with glucose and ketones strips. For in vivo inhibition of PPAR δ during fasting, rats were dosed with the specific inhibitor GSK3787 administered intra-peritoneally at 2 mg/kg every 12 h for 48 h, starting with food removal.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!