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Tlrl picw

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Tlrl-picw is a laboratory equipment product offered by Merck Group. It serves as a core function for research purposes, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

Nigericin, by Merck Group (1 mentions) Sorafenib, by Merck Group (1 mentions) Recombinant ifn γ, by Thermo Fisher Scientific (1 mentions) Z devd fmk, by Santa Cruz Biotechnology (1 mentions) Z vad fmk, by Santa Cruz Biotechnology (1 mentions) Tlrl pgcn, by InvivoGen (1 mentions) Mcc950, by InvivoGen (1 mentions) Ht dna, by Merck Group (1 mentions) Rnase a t1 cocktail, by Thermo Fisher Scientific (1 mentions) S1 nuclease, by Thermo Fisher Scientific (1 mentions) Dnase 1, by Bio-Rad (1 mentions) Nu7441, by Bio-Techne (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) N acetyl cysteine (nac), by Merck Group (1 mentions) Pam3csk4, by InvivoGen (1 mentions) Halofuginone, by Santa Cruz Biotechnology (1 mentions) Forskolin, by Cayman Chemical (1 mentions) Bafilomycin, by Merck Group (1 mentions) Prostaglandin e2 pge2, by Merck Group (1 mentions) Tlrl r848, by InvivoGen (1 mentions)

2 protocols using tlrl picw

1

Immune Activation Protocol Reagents

Cited 1 time
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The following chemicals and drugs were utilized in this study: Talabostat/VbP (MCE #HY-13233A), Lipofectamine 2000 (Invitrogen #11668019), double-stranded alternating copolymer poly(dA:dT) (pdAdT) (Sigma #P0883), poly(I:C) (pIC) (Invivogen #tlrl-picw), HT-DNA (Sigma #D6898), double-stranded homopolymer poly(dA):poly(dT) (Sigma #P9764), poly(dG:dC) (Invivogen #tlrl-pgcn), PAM3CSK4 (Invivogen #tlrl-pms), nigericin (Sigma #N7143), diABZI (MCE #HY-112921B), ANS (MCE #HY-18982), H2O2 (Sigma #H1009), MG-132 (MCE #HY-13259), Bortezomib (MCE #HY-10227), MCC950 (Invivogen #inh-mcc), z-VAD-FMK (Santa Cruz #sc-3067), z-DEVD-FMK (Santa Cruz #sc-311558), H-151 (MCE #HY-112693), NAC (Sigma #A9165), KU-44933 (Santa Cruz #sc-202963), NU-7441 (Tocris #3712), Sorafenib (Sigma #SML2633), PLX-4720 (MCE #HY-51424), Doramapimod (MCE #HY-10320), SB-202190 (MCE #HY-10295), and RNA Polymerase III inhibitor (Sigma #557403). gDNA was isolated from the genomic DNA of HEK293T cells. ISD was synthesized from custom oligos as previously described (55 (link)). Recombinant IFNγ was purchased from Peprotech (#300-02). DNase I (Bio-Rad #7326828), S1 Nuclease (Thermofisher #EN0321), and RNase A/T1 Cocktail (Thermofisher #AM2286) were purchased from the indicated vendors. VACV Copenhagen strain WT and ΔF1L were a kind gift of John Bell (56 (link)) and titered by plaque assay.
Zhou J.Y., Sarkar M.K., Okamura K., Harris J.E., Gudjonsson J.E, & Fitzgerald K.A. (2023). Activation of the NLRP1 inflammasome in human keratinocytes by the dsDNA mimetic poly(dA:dT). Proceedings of the National Academy of Sciences of the United States of America, 120(5), e2213777120.
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2

Innate Immune Stimuli Combination Protocol

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300 ng/mL Pam3Cys-SKKKK (EMC-microcollections L2000), 20 μg/mL LMW polyinosinicpolycytidylic acid (InvivoGen tlrl-picw), 100 ng/mL lipopolysaccharides E. coli 055:B5 (Sigma L6529), 5 μg/mL FLA (B. subtilis) ~32 kDa Gram-positive flagellin (InvivoGen FLA-BS), 10 μg/mL R848 imidazoquinoline compound (InvivoGen tlrl-r848), 5 μM CpG (ODN 1826) unmethylated CpG dinucleotides (InvivoGen tlrl-1826), 10 μg/mL muramyl dipeptide (InvivoGen tlrl-mdp), 10 μg/mL synthetic analog of trehalose-6,6- dimycolate (TDB, Trehalose-6,6-dibehenate) (InvivoGen tlrl-tdb), MOI ~10 Sendai virus (Sendai strain: Cantell) (ATCC VR-907), 5 μg/mL zymosan (S. cerevisiae) (Sigma Z4250), 20 μg/mL HT-DNA (herring testis DNA) (Trevigen). DMSO (Sigma), 10 μM forskolin (Cayman Chemical), 25 μM tert-Butylhydroquinone (tBHQ) (Sigma), 5 μM prostaglandin E2 (pGE2) (Sigma), 1 μM torin (Axon Medchem), 10 μM halofuginone (Santa Cruz Biotechnology), 100 nM bafilomycin (Sigma), 20 μM ZVAD pan-caspase inhibitor (Calbiochem), caspase I inhibitor IV (Calbiochem), 5 μg/mL brefeldin A, 5 μg/mL tunicamycin (Sigma).
O'Connell D.J., Kolde R., Sooknah M., Graham D.B., Sundberg T.B., Latorre I., Mikkelsen T.S, & Xavier R.J. (2016). Simultaneous Pathway Activity Inference and Gene Expression Analysis Using RNA Sequencing. Cell systems, 2(5), 323-334.
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