Lyovec lyec 12

All pre-designed shRNA constructs in a lentiviral format were purchased from Sigma-Aldrich, as described in Key resources table. We seeded 1 × 10⁵ cells in each well of a 12-well plate in 500 μL of complete medium and transduced them by lentiviral vectors at an MOI of 10:1. Transduction was carried out in the presence of polybrene (8 μg/mL; TR-1003-G, Sigma-Aldrich). After recovering with complete culture medium, puromycin (5 μg/mL; ant-pr-1, InvivoGen) was used for the selection of transduced cells. STING1 expression plasmid (SC321845, OriGene Technologies) was transfected into THP1 cells (1 × 10⁶) using Lipofectamine 3000 reagent (L3000-015, Invitrogen) according to manufacturer’s instruction. LyoVec (lyec-12, InvivoGen) was used as a nucleic acid complexing agent to facilitate the cellular entry of RNA or DNA-based oligonucleotides.

Trichloroacetic acids (T104257) was from Aladdin. Poly (I:C) (P9582) was purchased from Sigma Aldrich. Poly (dA:dT) naked (tlrl-patn) and LyoVec™(lyec-12) were purchased from InvivoGen. Human IL-2 (AF-200-02-1000) was from PeproTech. Fludarabine (NSC 118218) was purchased selleck.