5-PCF2Am-InsP5 (CF2) was synthesized as previously described [21] (link). 5-InsP7 and 5-PCP-InsP5 (5-PCP) synthesized using similar methods to those previously described [22] (link), [23] (link), [24] (link), [25] . All synthetic compounds were purified by ion-exchange and/or RP-18 chromatography and were fully characterized by 1H, 31 P, and 13 C nuclear magnetic resonance spectroscopy.
Anti-IP6K1, anti-myc, anti-coronin 1B, anti-Arp3, anti-cadherin antibodies were from Santa Cruz Biotechnology. Anti-Arp2, anti-p34 antibodies were from Bethyl Laboratories. Anti-flag, anti-vinculin antibodies were from Sigma-Aldrich. Anti-α-actinin, anti-FAK, anti-paxillin, anti-phospho-paxillin, anti-β-actin antibodies were from Cell Signaling Technology. Anti-phospho-FAK (Y397) antibody was from Abcam. Anti-GST antibody was from Proteintech.
HEK293, HEK293T/17 cell lines were from ATCC, HUVECs were from Lonza.
Anti-IP6K1, anti-myc, anti-coronin 1B, anti-Arp3, anti-cadherin antibodies were from Santa Cruz Biotechnology. Anti-Arp2, anti-p34 antibodies were from Bethyl Laboratories. Anti-flag, anti-vinculin antibodies were from Sigma-Aldrich. Anti-α-actinin, anti-FAK, anti-paxillin, anti-phospho-paxillin, anti-β-actin antibodies were from Cell Signaling Technology. Anti-phospho-FAK (Y397) antibody was from Abcam. Anti-GST antibody was from Proteintech.
HEK293, HEK293T/17 cell lines were from ATCC, HUVECs were from Lonza.