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11 protocols using ingeo 4043d

1

Characterization of Hemp-PLA Composites

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Hemp fibers (Cannabis sativa, Tisza, Hungary) grown in Saaremaa, Estonia were used. Their properties have been characterized and described in previous work [13 ]. The stems were industrially decorticated by a mechanical process. PLA fibers (IngeoTM 4043D) from NatureWorks (Minnetonka, MN, USA) were used for the research. The polymer was 60 mm long, with a round cross-section, a finesse of 6.7 dtex and a density of 1.24 g cm−3.
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2

Polylactic Acid Composite with Activated Charcoal and Silver

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Polylactic acid (PLA) (IngeoTM 4043D) with 94% L-lactide and 6% D-lactide was provided by Natureworks LLC (Minnetonka, MN, USA) (molecular weight (Mw) of 1.5 × 105 g/mol and polydispersity index (PDI) of 1.81). The melt flow index and density of PLA are 6.0 g/10 min and 1.24 g/cm3, respectively. Activated charcoal was purchased from Sigma-Aldrich Inc. (Lyon, France). Silver nitrate (AgNO3) was supplied by Fisher Scientific Co., Ltd. (Loughboroug, UK). Tungsten (W) and silver (Ag) electrodes (diameter of 1 mm, 99.95% purity) were obtained from The Nilaco Corporation, Japan. Chloroform (CHCl3) and hexane (C6H8) were analytical-grade and bought from RCI Labscan Co., Ltd. (Bangkok, Thailand). Palm oil was provided by Oleen Co., Ltd. (Samutsakhon, Thailand). Escherichia coli strain TISTR 527 and Stapphylococcus aureus strain TISTR 2329 were supplied by the Microbiological Resource Center, Thailand Institute of Scientific and Technological Research (TISTR). Bacteriological-grade beef extract, peptone and agar powder were purchased from HiMedia Laboratory Co., Ltd. (Maharashtra, India).
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3

Biobased and Biodegradable Polymer Composite

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Polylactic acid (PLA) Ingeo 4043D, with a content of 98% L-lactide, an average molecular weight (Mw) of 111 kDa, and a density of 1.24 g/cm3 was purchased from NatureWorks (Minnetonka, MN, USA). PHB powder with Mw 490 kDa and a density of 1.25 g/cm3 was purchased from Biomer (Schwalbach am Taunus, Germany) and acetyl tributyl citrate (ABC) plasticizer from Sigma-Aldrich (Burlington, MA, USA). Microcrystalline cellulose (MCC) powder with a mean particle size of 20 µm was supplied by Sigma-Aldrich (Schnelldorf, Germany). Mixed softwood and hardwood waste was collected from wood exploitations and timber factories. Sulfuric acid 96% from Fluka (Buchs, Switzerland) was used for the treatment of MCC.
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4

Chitin Nanocrystal Reinforced PLA Composites

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Polylactic acid (PLA) in pellet form (Ingeo 4043D) from NatureWorks, (Minnetonka, MN, USA) was used as the matrix. Chitin powder from shrimp shell was purchased from Sigma-Aldrich (grade C7170 (Stockholm, Sweden) and used as a starting material for the isolation of ChNCs. HCl (ACS reagent, 37%) for acid hydrolysis was purchased from Merck (Darmstadt, Germany). TEC (Mw: 276.3 g/mol) in liquid form was purchased from VWR (Stockholm, Sweden), and ethanol (99.5%) was purchased from Solveco (Stockholm, Sweden).
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5

Polymer Blends with Natural Antioxidants

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The object of the research was to study polymer blends based on polylactide (PLA) and polyhydroxybutyrate (P(3.4 HB) contained selected natural antioxidants. Polylactide (PLA), Ingeo 4043D, was obtained from NatureWorks LLC (Minnetonka, MN, USA). This material with a density of 1.25 g/cm3 is characterized by glass transition of about 55–60 °C. The melting point of this polymer is 145–160 °C and the melt flow index equals 6 g/10 min. Poly(hydroxybutyrate) (P(3.4 HB)) containing 12 mol% 4-hydroxybutyrate was obtained from Simag Holdings LTD (Hong Kong, China). The density of this polymer is 1.25 g/cm3, its melt flow index equals 18 g/10 min and the melting point is 170 °C. Different natural antioxidants, including lignin (alkali, low sulfonate content lignin, kraft, pH: 10.5 (3 wt %)), flavone (Mw = 222.24 g/mol, mp: 94–97 °C, ≥99.0%) and trans-chalcone (Mw = 208.26 g/mol, mp: 55–57 °C, 97%), used as stabilizers, were obtained from Sigma-Aldrich (Saint Louis, MI, USA).
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6

PLA-based Polymer Synthesis

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Polylactic acid (PLA) Ingeo 4043D, with an L-lactide content of 98%, an average molecular weight of 111 kDa, and a density of 1.24 g/cm3, was supplied by Nature Works (Blair, NE, USA). Sebacic acid (Se) (purity 99%) and titanium (IV) butoxide (TBT) (purity 97%) were purchased from Aldrich (Milwaukee, WI, USA), while 1,4-butanediol (BDO) (purity 99%) was procured from Merck Co. (Darmstadt, Germany). All chemicals were used as received without further purification.
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7

Fabrication of PLA-based Composites

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Polylactide (PLA)-type Ingeo 4043D was purchased from NatureWorks (Minnetonka, Minneapolis, MN, USA). Diatomaceous earth (Perma-Guard, Bountiful, UT, USA) was derived from diatomite deposits. Synthetic wax WTH-B microbeads were bought from WTH GmbH (Hamburg, Germany).
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8

Doxorubicin-loaded PLA/PVP Nanocomposites

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Polylactic acid (PLA) resin (Ingeo 4043D, NatureWorks), 98% L-lactide, with weight average molecular weight of 111 kg/mol was purchased from NatureWorks (Minnetonka, MN, USA). Polyvinylpyrrolidone (PVP) with average molecular weight of 1300 kg/mol was purchased from Alfa Aesar (Haverhill, MA, USA). Dimethyl sulfoxide (DMSO) and dimethylformamide (DMF) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Doxorubicin hydrochloride (99%, Dox-HCl) was purchased from MedKoo Biosciences (Morrisville, NC, USA). Solvents, phosphate buffered saline (PBS, pH 7.4) were purchased from Thermo Fisher (Waltham, MA, USA), and other chemicals were purchased from Fisher Scientific (Hampton, NH, USA).
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9

Polylactide Composite with Grape Seed Extract

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Unless otherwise specified, all chemicals and reagents were purchased from Sigma Aldrich and used as received. Polylactide (PLA) pellets were purchased from NatureWorks, LLC (Ingeo 4043D, Mn ∼100,000Da, and Mw ∼150,000Da). Grape seed extract (GSE) was 95% proanthocyanidins-rich Vitis vinifera (MegaNatural™ gold grape seed extract, California, USA).
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10

Biopolymer-Based Antimicrobial Composite

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Polymer matrixes employed in this research: epoxidized natural rubber (ENR) (Epoxyprene ENR-50; 50 mol% epoxidation) obtained from Kumpulan Guthrie Berhad (Kuala Lumpur, Malaysia) and poly(lactic acid) (PLA), Ingeo 4043D purchased from Nature Works (Minnetonka, MN, USA). Lauric acid (97% purity), 1,2-dimethylimidazole (highest available purity) provided by Sigma-Aldrich (Darmstadt, Germany) and elastin hydrolysate purchased from Proteina (Lodz, Poland) were used as a cross-linking system. Natural additives, namely, δ-tocopherol (δ-TF), CM—curcumin (CM), β-carotene (β-CT), quercetin (QU), of the highest available purities were purchased from Sigma-Aldrich (Darmstadt, Germany). Structures of natural additives are shown in Figure 1.
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