Vancomycin and oxacillin were purchased from Sigma‐Aldrich (St Louis, MO, USA). Diclofenac and daptomycin were purchased from Dalian Meilun Biotechnology Co., Ltd (Dalian, China). All drugs were made up to10 mg mL−1 stocks in DMSO or ddH2O. For assays with daptomycin, the medium or buffer was supplemented with 50 µg mL−1 CaCl2.
Daptomycin
Manufactured by Meilun
Sourced in China
Daptomycin is a cyclic lipopeptide antibiotic used in the treatment of certain bacterial infections. It functions by disrupting the cell membrane of Gram-positive bacteria, leading to cell death.
Automatically generated - may contain errors
Lab products found in correlation
Cefazolin, by Meilun (2 mentions)
Erythromycin, by Meilun (2 mentions)
Tigecycline, by Meilun (2 mentions)
Vancomycin, by Merck Group (1 mentions)
Oxacillin, by Merck Group (1 mentions)
Linezolid, by Selleck Chemicals (1 mentions)
Trimethoprim sulfamethoxazole, by Merck Group (1 mentions)
Gentamicin, by Meilun (1 mentions)
Rifampicin, by Meilun (1 mentions)
Clindamycin, by Meilun (1 mentions)
Moxifloxacin, by Meilun (1 mentions)
Linezolid, by Meilun (1 mentions)
Glucose 6 phosphate (g6p), by Merck Group (1 mentions)
Vancomycin, by Meilun (1 mentions)
Amikacin, by Meilun (1 mentions)
Tetracycline, by Meilun (1 mentions)
Levofloxacin, by Meilun (1 mentions)
Oxacillin, by Meilun (1 mentions)
4 protocols using daptomycin
1
Antibiotic Preparation for Assays
2
Daptomycin-Loaded PEGylated Liposomes
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Daptomycin was purchased from Meilun Biotech Co. (Dalian, China). NHS–PEG3400–DSPE was supplied by Nanocs Corp. (New York, NY, USA). Soybean phospholipids (SPC) were from Aladdin Bio-Chem Technology Co., Ltd. (Shanghai, China). m-Polyethylene glycol2000 (mPEG2000)–DSPE was obtained from AVT pharmaceuticals Co., Ltd. (Shanghai, China). 1,1′-Dioctadecyl-3,3,3,3-tetramethylindotricarbocyanineiodide (DiR) was supplied by Invitrogen Corp. (Life Technologies, Carlsbad, CA, USA). Fluoresceinamine isomer II (FAM) was from Sigma-Aldrich (St. Louis, MO, USA). The other reagents were all of analytical grade.
MRSA252 was incubated in tryptic soy broth (Nissui Pharmaceuticals, Japan) at 37 °C. Six-week-old male/female Kunming (KM) mice weighing 20±2 g were purchased from the experimental animal center of Third Military Medical University (Chongqing, China) and kept in regulated conditions. All animal experiments were performed in accordance with the guidelines approved by the ethics committee of the College of Pharmaceutical Sciences, Southwest University, China.
MRSA252 was incubated in tryptic soy broth (Nissui Pharmaceuticals, Japan) at 37 °C. Six-week-old male/female Kunming (KM) mice weighing 20±2 g were purchased from the experimental animal center of Third Military Medical University (Chongqing, China) and kept in regulated conditions. All animal experiments were performed in accordance with the guidelines approved by the ethics committee of the College of Pharmaceutical Sciences, Southwest University, China.
3
Antimicrobial Agents Evaluation Protocol
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The antimicrobials utilized in this work were cefazolin, gentamicin, erythromycin, tigecycline, rifampicin, daptomycin (purchased from Dalian Meilun Biotech, Dalin, China), ciprofloxacin, clindamycin, vancomycin (from the National Institutes for Food and Drug Control, Beijing, China), linezolid (Selleck Chemicals, Houston, TX, United States), and trimethoprim–sulfamethoxazole (Sigma–Aldrich, St Louis, MO, United States).
4
Antibiotic Susceptibility Testing Protocol
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Antibiotic agents (oxacillin, cefazolin, cefuroxime, clindamycin, erythromycin, levofloxacin, moxifloxacin, tetracycline, tigecycline, amikacin, fosfomycin, trimethoprim-sulfamethoxazole, linezolid, vancomycin, daptomycin, rifampin) were purchased from Dalian Meilun Biotech (Dalian, China). Glucose-6-phosphate was obtained from Sigma-Aldrich Co. (St Louis, MO, USA). tigecycline and daptomycin susceptibility test were done via broth dilution method according to CLSI recommendations. The MICs of the other 15 antibiotics were determined using the twofold serial agar dilution method.9
E. faecalis ATCC 29212 were used as quality-control strains.
E. faecalis ATCC 29212 were used as quality-control strains.
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