Protein a g plus agrose

Manufactured by Santa Cruz Biotechnology

Protein A/G PLUS-Agarose is a protein affinity resin designed for the purification of immunoglobulins (IgGs) from various sources. It consists of recombinant Protein A and Protein G covalently coupled to high-performance agarose beads, providing a versatile platform for the capture and isolation of IgGs.

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Lab products found in correlation

Ab16046, by Santa Cruz Biotechnology (1 mentions) Ab16046, by Merck Group (1 mentions) Abe68, by Abcam (1 mentions) Anti myc antibody, by Roche (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)

3 protocols using protein a g plus agrose

Immunoprecipitation of Cellular Proteins

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For immunoprecipitation, the cell lysates were incubated with specific primary antibodies for 4 h at 4 °C. Then protein A/G PLUS-Agrose (Santa cruz) were added to antibody containing cell lysates and incubated for 2 h at 4 °C. Immunoprecipitated proteins were loaded to SDS-PAGE.

Yu J.E., Yeo I.J., Yoo S.S., Kim S.H., Son D.J., Yun J., Han S.B, & Hong J.T. (2023). Induction of ER stress-mediated apoptosis through SOD1 upregulation by deficiency of CHI3L1 inhibits lung metastasis. Theranostics, 13(8), 2693-2709.

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Co-Immunoprecipitation Assay Protocol

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Co-IP assays were performed by transfecting HEK-293T cells with the indicated plasmids using lipofectamine™ 2000 (Invitrogen). After 48 hours, cells were harvested. Cell extracts were subjected to immunoprecipitation with either the anti-Myc antibody (1/2000, Roche, 11667149001), anti-His (1/2000, GenScript, A00174) or anti-Flag (1/2000, Agilent Technologies, 200472) antibody as indicated for overnight at 4°C. The antibody was coupled to protein A/G PLUS-Agrose (Santa Cruze, sc-2003). The immunoprecipitates were washed eight times with washing buffer and analyzed by SDS-PAGE and immunoblotting with antibody as indicated. For in vivo analysis, Nuclear extracts from E13.5 limb were immunoprecipitated with anti-Foxp1 antibody (Millipore, ABE68), anti-Foxp2 antibody (Abcam, ab16046) or IgG (Santa Cruze, sc-2027), and then blotted with anti-Foxp1 (1/1000, Millipore, ABE68), anti-Foxp2 (1/2000, Abcam, ab16046), anti-Foxp4 antibody (1/1000, Milipore, ABE74) and anti-Runx2 antibody (1/1000, Santa Cruze, sc-10758) as indicated.

Zhao H., Zhou W., Yao Z., Wan Y., Cao J., Zhang L., Zhao J., Li H., Zhou R., Li B., Wei G., Zhang Z., French C.A., Dekker J.D., Yang Y., Fisher S.E., lucker H.O, & Guo X. (2014). Foxp1/2/4 regulate endochondral ossification as a suppresser complex. Developmental biology, 398(2), 242-254.

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Extraction and Detection of Membrane Proteins

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Proteins of cellular membrane were extracted using the ProteoExtract ® Transmembrane Protein Extraction Kit (NOVAGEN). Total 500 μg of membrane proteins were used for immunoprecipitation with an anti AT 1 -R or LOX-1 antibody.
The immunecomplexes were precipitated with protein A/G PLUS-Agrose (Santa Cruz Biotechnology) and subjected to the SDS-PAGE for detecting amounts of LOX-1 or AT 1 -R.

Li L., Zhou N., Kang L., Wang Q., Wu J., Wang X., Yang C., Zhang G., Chen Y., Jiang H., Chen R., Yang X., Sun A., Gong H., Ren J., Aikawa H., Komuro I., Ge J., Yang C., & Zou Y. (2020). Oxidized LDL but not angiotensin II induces the interaction between LOX-1 and AT1 receptors.

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