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5 n n dimethyl amiloride hydrochloride dma

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5-(N,N-Dimethyl)-amiloride hydrochloride (DMA) is a chemical compound used as a laboratory reagent. It functions as a sodium-proton exchanger inhibitor.

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Lab products found in correlation

Anti hsp70, by System Biosciences (3 mentions) Anti cd9, by System Biosciences (3 mentions) Anti cd63, by System Biosciences (3 mentions) Anti cd81, by System Biosciences (3 mentions) Rapamycin, by Merck Group (2 mentions) Gw4869, by Merck Group (2 mentions) Exoquick kit, by System Biosciences (2 mentions) Afatinib, by Selleck Chemicals (1 mentions) Ml141, by Bio-Techne (1 mentions) Nsc23766, by Bio-Techne (1 mentions) Epidermal growth factor (egf), by Merck Group (1 mentions) Cytochalasin d, by Bio-Techne (1 mentions) Zcl278, by Bio-Techne (1 mentions) Casin, by Bio-Techne (1 mentions) 5 n ethyl n isopropyl amiloride eipa, by Cayman Chemical (1 mentions) Wiskostatin, by Bio-Techne (1 mentions) Ck869, by Bio-Techne (1 mentions) Ck666, by Bio-Techne (1 mentions) U0126, by Merck Group (1 mentions) Ly294002, by Merck Group (1 mentions)

4 protocols using 5 n n dimethyl amiloride hydrochloride dma

1

Inhibitors of Actin Dynamics in Cell Biology

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All ligands, chemical compounds, and inhibitors used in this manuscript were cell biological grade: EGF (Sigma-Aldrich, cat# E9644), NH4Cl (Sigma-Aldrich, cat# A0171), NSC23766 (TOCRIS, cat# 2161), Casin (TOCRIS, cat# 5050), ZCL278 (TOCRIS, cat# 4794), ML141 (TOCRIS, cat# 4266), PBP10 (Calbiochem, cat# 529625), Rapamycin (Sigma-Aldrich, cat# S-015), U0126 (Sigma-Aldrich, cat# 19–147), Quercetagetin (Calbiochem, cat# 551590), Afatinib (Selleckchem, cat# S1011), 5-(N,N-Dimethyl)-amiloride hydrochloride (DMA, Sigma-Aldrich, cat# A4562), 5-(N-ethyl-N-isopropyl)-amiloride (EIPA, Cayman Chemical Company, cat# 14406), Wiskostatin (TOCRIS, cat# 4434), 187-1, N-WASP inhibitor (TOCRIS, cat# 2067), CK869 (TOCRIS, cat# 4984), CK666 (TOCRIS, cat# 3950), Cytochalasin D (TOCRIS, cat# 1233), and LY294002 (Sigma-Aldrich, cat# L9908).
Endo Y., Hickerson B.T., Ilyushina N.A., Mohan N., Peng H., Takeda K., Donnelly R.P, & Wu W.J. (2022). Identification of a pharmacological approach to reduce ACE2 expression and development of an in vitro COVID-19 viral entry model. Journal of Virus Eradication, 8(4), 100307.
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2

Adipocyte Differentiation Assay Protocol

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Minimum essential medium α (MEMα), Opti-MEM alpha, and fetal bovine serum (FBS) were obtained from Gibco (Carlsbad, CA, USA). Total exosome isolation reagent, and lipofectamine RNAiMAX was purchased from Invitrogen (Foster city, CA, USA) and 5-(N,N-Dimethyl) amiloride hydrochloride (DMA), insulin, 3-isobutyl-1-methylxanthine (IBMX), dexamethasone (DEXA), anti-TRPML1, and oil red O dye were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Antibodies against PPARγ was purchased from Santa-Cruz Biotechnology, Inc. (CA, USA). Antibodies against C/EBPα, and β-actin were obtained from Cell Signaling Technology (Beverly, MA, USA). Exosome-depleted FBS and exosome marker antibodies including anti-CD9, anti-CD81, anti-CD63, and anti-Hsp70 were procured from System Biosciences (Palo Alto, CA, USA). Anti-LAMP1 antibody was purchased from Abcam (Cambridge, MA, USA).
Kim M.S., Muallem S., Kim S.H., Kwon K.B, & Kim M.S. (2019). Exosomal release through TRPML1-mediated lysosomal exocytosis is required for adipogenesis. Biochemical and biophysical research communications, 510(3), 409-415.
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3

Extracellular Vesicle Characterization in Prostate Cancer Cell Lines

Cited 1 time
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Human PCa LNCaP, 22Rv1, PC3, and PWR-1E cells were purchased from ATCC (Manassas, VA). MDA PCa 2b cells were from Dr. Balaji (Wake Forest School of Medicine). RC77T/E and RC77N/E cell lines characterization was reported by Theodore et al.64 (link) E006AA-hT cells were from Dr. Koochekpour (Roswell Park Memorial Institute). ExoQuick™ Kit was from System Biosciences (Mountain View, CA). CD63 (#ab59479), actin (#ab8226), and Rab5 (#ab18211) antibodies were from Abcam (Cambridge, MA). Secondary antibodies anti-rabbit IgG (H + L) F(ab’)2 fragment (Alexa Fluor® 555 Conjugate) (#4413 S), anti-mouse IgG (H + L) F(ab’)2 fragment (Alexa Fluor® 647 Conjugate) (#4410 S), and anti-mouse IgG (H + L) F(ab’)2 fragment (Alexa Fluor® 488 Conjugate) (#4408 S) were from Cell Signaling Technology (Danvers, MA). RPMI1640, penicillin and streptomycin, 0.25% trypsin, fetal bovine serum (FBS), and heat-inactivated FBS were from Gibco Laboratories (Gaithersburg, MD). Halt™ Protease and Phosphatase Inhibitor Cocktail, Keratinocyte-SFM and BRFF-HPC1™ media were from Thermo Fisher Scientific (Waltham, MA). DC™ Protein Assay kit was from Bio-Rad (Hercules, CA). The Poly- L-Lysine, Lactate Assay Kit II, 5-(N,N-Dimethyl)amiloride hydrochloride (DMA) and GW4869 were from Sigma-Aldrich (St. Louis, MO).
Panigrahi G.K., Praharaj P.P., Peak T.C., Long J., Singh R., Rhim J.S., Abd Elmageed Z.Y, & Deep G. (2018). Hypoxia-induced exosome secretion promotes survival of African-American and Caucasian prostate cancer cells. Scientific Reports, 8, 3853.
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4

In vitro Screening of FDA-Approved Drugs

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An FDA-approved drug library was purchased from Selleckchem (L1300) and used for in vitro screening study. The following chemicals were purchased from Sigma Aldrich, including Sulfisoxazole (SFX, S6377), 5-(N,N-dimethyl)amiloride hydrochloride (DMA, A4562), GW4869 (D1692), Rapamycin (R8781), FTY720 (SML0700), ET1 (E7764), BQ123 (B150), PD156707 (PZ0141), zibotentan (SML1550), and Bafilomycin (B1793). ET2 (#1164) was obtained from Tocris Bioscience. Docetaxel (DOC) was a gift from professor Keon-Wook Kang (Seoul National University).
Im E.J., Lee C.H., Moon P.G., Rangaswamy G.G., Lee B., Lee J.M., Lee J.C., Jee J.G., Bae J.S., Kwon T.K., Kang K.W., Jeong M.S., Lee J.E., Jung H.S., Ro H.J., Jun S., Kang W., Seo S.Y., Cho Y.E., Song B.J, & Baek M.C. (2019). Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A. Nature Communications, 10, 1387.
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