The immortalized normal human urothelial cell line SV-HUC-1 and human bladder urothelial carcinoma (BLCA) cell lines T24 and 5637, and human proximal tubular epithelial cell line HK-2 and human clear cell renal cell carcinoma (ccRCC) cell lines 786-O and caki-1, and human normal prostatic epithelial cells RWPE-1 and human prostate cancer (PCa) cell lines PC-3 and DU145 were all purchased from Procell Life Science and Technology Co., Ltd. (Wuhan, China). SV-HUC-1 and PC-3 cells were cultured in Ham’s F-12K medium (Procell) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. T24, HK-2, caki-1, and DU145 cells were cultured in DMEM medium (Procell) supplemented with 10% FBS and 1% penicillin/streptomycin. RWPE-1 cells were cultured in complete medium of human prostatic epithelial cells (Procell). Furthermore, 5637 and 786-O cells were cultured in RPMI 1640 medium (Procell) supplemented with 10% FBS and 1% penicillin/streptomycin. All the cell lines were cultured in an incubator with 5% CO2 at 37 °C. Elesclomol (Synta Pharmaceuticals, Lexington, MA, United States) was diluted in a cell culture medium at 100 nmol/L concentration.
Sv huc 1
Manufactured by Procell
Sourced in China
The SV-HUC-1 is a laboratory instrument that performs cell harvesting. It is designed to efficiently detach adherent cells from culture vessels, enabling their collection for further analysis or processing.
Automatically generated - may contain errors
Lab products found in correlation
Umuc3, by Procell (6 mentions)
Rpmi 1640 medium, by Procell (5 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (4 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions)
Rpmi 1640, by Thermo Fisher Scientific (3 mentions)
Dmem medium, by Procell (2 mentions)
Caki 1, by Procell (2 mentions)
Rwpe 1, by Procell (2 mentions)
Du145, by Procell (2 mentions)
Ham s f 12k medium, by Procell (2 mentions)
Sv huc 1, by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by Procell (2 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)
Rt112, by Procell (2 mentions)
Umuc3, by American Type Culture Collection (2 mentions)
Sv huc 1, by American Type Culture Collection (2 mentions)
Um uc 3, by Thermo Fisher Scientific (2 mentions)
Ham s f 12k, by Thermo Fisher Scientific (1 mentions)
18 protocols using sv huc 1
1
Cell Culture Conditions for Cancer and Normal Cell Lines
2
Culturing Human Cell Lines for Research
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The human proximal tubular epithelial cell line HK-2 and human ccRCC cell lines 786-O and Caki-1, immortalized normal human urothelial cell line SV-HUC-1 and human bladder urothelial carcinoma cell lines T24 and 5637, and human normal prostatic epithelial cell line RWPE-1 and human PCa cell lines PC-3 and DU145 were all purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China). HK-2, Caki-1, T24, and DU145 cells were cultured in DMEM medium (Procell) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. Furthermore, 786-O and 5637 cells were cultured in RPMI 1640 medium (Procell) supplemented with 10% FBS and 1% penicillin/streptomycin. SV-HUC-1 and PC-3 cells were cultured in Ham’s F-12K medium (Procell) supplemented with 10% FBS and 1% penicillin/streptomycin. RWPE-1 cells were cultured in complete medium of human prostatic epithelial cells (Procell). All the cell lines were cultured in an incubator with 5% CO2 at 37 °C.
3
Cell Culture and MED Compound Preparation
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SV-HUC-1 (RRID:CVCL_3798), T24 (RRID: CVCL_0554) and EJ-1 (RRIDCVCL_2893) cell lines, which were obtained from Procell Life Science &Technology Co., Ltd. (China), were cultured in 1640 medium containing 10 % fetal bovine serum (Gibco, USA) and 1 % penicillin-streptomycin (Gibco) in an incubator containing 5 % CO 2 at 37 °C. MED (C 16 H 14 O 6 ) with ≥ 98 % of purity was brought from Desite (CAS NO: 32383-76-9, Chengdu, China), and dissolved in dimethyl sulfoxide (DMSO) to obtain the mother liquor.
4
Bladder Cancer Cell Line Culture
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Human bladder cancer cells T24, UMUC3, and J82 and human normal bladder epithelium cell line SVHUC-1 were purchased from Procell (Procell Life Science & Technology Co., Ltd). Cells were cultured in RPMI-1640 medium (Invitrogen) mixed with 10% FBS. The incubator was set in a water-saturated atmosphere with 5% CO2 at 37 °C.
5
Bladder Cancer Cell Culture Protocols
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Human bladder carcinoma SW780 (Grade I) and 5637 (Grade II) cells, as well as human bladder epithelial cell SV-HUC-1, were obtained from Procell Life Science & Technology Co., Ltd. (Wuhan, China). All the above cell lines were certified and routinely tested for mycoplasma contamination before use. For SV-HUC-1 cells, the culture medium consisted of Ham’s F-12K supplemented with 10% FBS (GIBCO, USA) and 1% Penicillin/Streptomycin (Invitrogen, USA), while for 5637 cells, RPMI-1640 supplemented with 10% FBS (GIBCO, USA) and 1% Penicillin/Streptomycin (Invitrogen, USA) were used and grew under 95% air and 5% CO2 at 37°C. SW780 cells were cultured in Leibovitz’s L-15 supplemented with 10% FBS (GIBCO, USA) and 1% Penicillin/Streptomycin (Invitrogen, USA) under 100% air at 37°C.
6
Bladder Cancer Cell Line Cultivation
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Human normal bladder epithelial cell line SV-HUC-1 was purchased from ATCC. The human urinary bladder transitional cell carcinoma cell lines T24, RT4, UMUC3 and 5637 were purchased from ATCC. EJ cells were obtained from the Institute of Biochemistry and Cell Biology of Chinese Academy of Sciences (Shanghai, China). CDDP-resistant cell lines (EJ-CDDP) were derived from our previous studies [15 (link)]. All cells were maintained in a humidified incubator with 5% CO2 at 37℃. The SV-HUC-1 cells were cultured in F12K medium (Procell, China). The T24, EJ, RT4, 5637 and EJ-CDDP cells were cultured in RPMI 1640 medium (Procell, China). The UMUC3 cells were cultured in DMEM (Procell, China). All medium was supplemented with 10% FBS (Vazyme, China) and 1% penicillin/streptomycin (Procell, China). All cell lines were confirmed negative for Mycoplasma contamination. Cisplatin (CDDP) and actinomycin D (ActD) were purchased from Sigma-Aldrich (USA) and solubilized in DMSO.
7
Cell Culture Protocols for Bladder and Kidney
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The BCa cell lines (T24, 5637, and UM-UC-3) and the human embryonic kidney cell line HEK-293 T were obtained from the cell bank of the Chinese Academy of Sciences (Shanghai, China). The normal urothelial cell line SV-HUC-1 was obtained from the Procell Life Science and Technology (Wuhan, China). These cell lines were separately cultured in McCoy's 5a (T24), RPMI 1640 (5637 and SV-HUC-1), and DMEM (UM-UC-3 and HEK-293 T) in an incubator at 37 °C, in the presence of 5% CO2. All the culture media were supplemented with 10% fetal bovine serum (FBS, Gibco, USA) and 1% antibiotics (penicillin/streptomycin) (Servicebio, China).
8
Bladder Cancer Cell Culture Protocol
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The human bladder cancer cell lines T24 and SV-HUC-1 was purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China). The 5637, J82, UMUC-3 and EJ-1 cells were obtained from Yuhuangding Hospital (Yantai, China). 5637, EJ-1, UM-UC-3 cells were cultured in RPMI1640 media (Procell, PM150110); J82 cells were cultured in MEM (NEAA) media (Procell, PM150410), and T24 cells were cultured in McCoy’s 5A media (PM150710). A total of 10% FBS (Gibco,1099141C) and 1% antibiotics (penicillin and streptomycin, Procell, PB180120) were added to the basic media. All the cells were cultured at 37°C with 5% CO2.
9
TFRC Silencing in Bladder Cancer Cells
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All cell lines were purchased from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). Two BLCA cell lines (J82 and UMUC3) were cultivated in DMEM (Gibco) containing 10% fetal bovine serum, and one normal human urothelial cell line (SV-HUC1) were grown in SV-HUC-1 cell special medium (procell, Wuhan, China). Cultures were incubated at 37 °C with 5% CO2 in a humidified atmosphere. The TFRC si-RNA was designed and synthesized by Hanbio (Shanghai, China). Lipofectamine 2000 (Invitrogen, USA) was utilized as a transfection reagent according to the instructions. J82 and UMUC3 cell lines were transfected with siRNA (10 nmol/l) utilizing Lipofectamine 2000 (5 μl per well, Invitrogen, USA) according to the manufacturer's protocol.
10
Bladder Cancer Cell Line Culture
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Human bladder cancer cells T24, 5637, J82, RT112, EJ, TCCSUP, UM-UC-3, and human bladder immortalized epithelium cell line (SV-HUC-1) were purchased from Procell (Procell Life Science& Technology Co., Ltd). SV-HUC-1 cells were cultured in Ham’s F-12K medium. MEM mixed with 10% FBS (Excell) was used to cultivate the UM-UC-3 and the J82 line. RPMI1640 (Invitrogen) mixed with 10% FBS (Excell) was used to cultivate T24, 5637, RT112, EJ, TCCSUP, and those cell lines were all cultured in a water-saturated atmosphere with 5% CO2 at 37°C.
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