Huxuc 9004

Differentiation of UC-MSCs into adipocytes was performed using Mesenchymal Stem Cell Adipogenic Differentiation Medium (Cyagen, HUXUC-90031) according to the manufacturer's instruction. The Oil Red O staining was performed 17 days later. Differentiation of UC-MSCs into chondrocytes was performed using Mesenchymal Stem Cell Chondrogenic Differentiation Medium (Cyagen, HUXUC-9004) according to the manufacturer's instruction. The tissue samples were formalin-fixed and paraffin-embedded on day 18 and further underwent Alcian Blue staining. Differentiation of UC-MSCs into osteoblasts was performed using Mesenchymal Stem Cell Osteoblastic Differentiation Medium (Cyagen, HUXUC-90021) according to the manufacturer's instruction. The Alizarin Red staining was performed 17 days later.

The hUCMSCs were purchased from Haixing Biosciences (Suzhou, Jiangsu, China). The hUCMSCs were cultured in accordance with a previously described method[45 (link)]. Afterward, the adipogenic, osteogenic, chondrogenic capacity of these cells was performed. Passages 3–7 of hUCMSCs were cultured in osteogenic, adipogenic or chondrogenic differentiation medium (Cyagen, China, HUXUC-9004) as described by manufacturer. Oil red O staining, Alizarin red staining and Alcian blue staining were used to evaluate adipogenesis, osteogenesis, and chondrogenesis. Detection of hUCMSCs surface markers by a FACSVerse instrument (BD Bioscience, San Jose, CA, USA) is performed as follows. The hUCMSCs were stained with human anti-CD14, anti-CD19, anti-CD34, anti-CD45, anti-CD73, anti-CD90, anti-CD105, or anti-HLA-DR. Identical concentrations of PE-conjugated mouse IgG isotype antibodies were used as negative controls (all from BD Biosciences). Data were analyzed using the software FlowJo V10 (FlowJo).