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Goat anti rabbit igg conjugated with 18 nm colloidal gold particles

Manufactured by Jackson ImmunoResearch
Sourced in Panama

Goat anti-rabbit IgG conjugated with 18 nm colloidal gold particles. This product is a secondary antibody that binds to rabbit IgG antibodies and is labeled with 18 nm colloidal gold particles.

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3 protocols using goat anti rabbit igg conjugated with 18 nm colloidal gold particles

1

Immunogold Electron Microscopy of α-Synuclein and TDP-43

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A piece of thalamic fasciculus from formalin-fixed brain of MSA-13 was processed for immunogold electron microscopy (IEM) as described previously [44 (link)]. Rabbit polyclonal antibodies to α-synuclein [39 (link)] and TDP-43 (ProteinTech Group, Chicago, IL) were used. Goat anti-rabbit IgG conjugated with 18 nm colloidal gold particles was purchased from Jackson ImmunoResearch Laboratories (West Grove, PA). For TDP-43 IEM, thin sections collected on Formvar-coated nickel grids were treated with citrate buffer, pH 6, for 10 minutes at 95°C before antibody incubation. EM images were obtained with a Gatan 831 Orius digital camera fitted in a Philips 208S electron microscope, and processed using Photoshop software.
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2

Visualizing Recombinant and Patient Derived Poly(GA) Proteins

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To examine the filamentous structure of recombinant GST-(GA)50 proteins, recombinant GST or GST-(GA)50 proteins were diluted to 1 µg/µl in 20 mM Tris–HCl, pH 7.5, 50 mM KCl, 10 mM MgCl2 in a final volume of 30 µl. The samples were incubated at 30 °C for 6 h, and then diluted to 0.1 µg/µl by reaction buffer and loaded onto grids for regular EM analysis. For immuno-EM analysis, mouse monoclonal anti-GST antibody (1:20, Thermo Scientific) was used as primary antibody, and goat anti-mouse IgG conjugated with 6 nm colloidal gold particles (1:20, Jackson ImmunoResearch Laboratories) was used as the secondary antibody. To examine the filamentous structure of poly(GA) proteins in c9FTD/ALS patients, small pieces (1.5 × 1.5 × 1 mm) of cerebellar folia or hippocampus from formalin-fixed brains were dissected and processed for routine electron microscopy (EM) or post-embedding immunogold EM as previously described [34 (link)]. Rabbit polyclonal anti-poly(GA) antibody (1:50) was used as a primary antibody and goat anti-rabbit IgG conjugated with 18 nm colloidal gold particles (1:20, Jackson ImmunoResearch Laboratories) was used as the secondary antibody. Thin sections stained with uranyl acetate and lead citrate were examined with a Philips 208S electron microscope (FEI) fitted with a Gatan 831 Orius CCD camera (Gatan). Digital images were processed with Adobe Photoshop CS5 software.
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3

Visualizing poly(GA) protein fibrils in mouse brain

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To examine the filamentous structure of poly(GA) proteins in mouse brain, immunoEM was performed as previously described52 (link). Thin sections were pretreated with sodium citrate buffer, pH 6 at 90–95 °C for 10 min before immunolabeling. Rabbit polyclonal antibody to poly(GA) (1:150 in PBS) was used as the primary antibody, and goat anti-rabbit IgG conjugated with 18 nm colloidal gold particles (1:20, Jackson ImmunoResearch Laboratories) was used as the secondary antibody. Thin sections stained with uranyl acetate and lead citrate were examined with a Philips 208S electron microscopy (FEI) fitted with a Gatan 831 Orius charge-coupled device (CCD) camera (Gatan). Digital images were processed with Adobe Photoshop CS5 software.
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