E. coli LPS 0127:B8 with the lot activity of 3,000,000 U/mg, and 40 kDa fluorescein isothiocyanate (FITC)-dextran were purchased from Sigma (St. Louis, MO). The antibody recognizing VE-cadherin was from Cayman Chemical (Ann Harbor, MI); antibody to cleaved caspase-3 was from Cell Signaling (Beverly, MA); antibodies to Bcl-2 and Bad were from Santa Cruz Biotechnology (Dallas, TX); antibodies to phospho-Bad and Bim were from Cell Signaling (Beverly, MA), antibody to β-actin was from Sigma (St. Louis, MO). Carboxy-dichlorofluorescein diacetate (carboxy-DCFH-DA), and all reagents used for immunofluorescent staining were obtained from Invitrogen (Carlsbad, CA). All reagents for Flow Cytometry were from BD Biosciences (San Jose, CA).
Antibody to cleaved caspase 3
Manufactured by Cell Signaling Technology
Sourced in United Kingdom
The Antibody to cleaved caspase 3 is a lab equipment product that detects the presence of cleaved caspase 3, a protein involved in the apoptosis (programmed cell death) pathway. This antibody can be used to identify and quantify the levels of cleaved caspase 3 in various experimental settings.
Automatically generated - may contain errors
Lab products found in correlation
Lps e coli 0127 b8, by Merck Group (1 mentions)
Antibody to β actin, by Merck Group (1 mentions)
Annexin 5 fluorescein isothiocyanate fitc apoptosis detection kit, by BD (1 mentions)
Fecl2 4h2o, by Sinopharm (1 mentions)
Cck 8, by Dojindo Laboratories (1 mentions)
Fecl3 6h2o, by Sinopharm (1 mentions)
Nitric acid, by Sinopharm (1 mentions)
Ammonium hydroxide, by Sinopharm (1 mentions)
Pharmingen brdu flow kit, by BD (1 mentions)
Dnase, by Roche (1 mentions)
Liberase, by Roche (1 mentions)
Scanscope at2, by Leica (1 mentions)
Dako autostainer, by Agilent Technologies (1 mentions)
4 protocols using antibody to cleaved caspase 3
1
Endothelial Cell Apoptosis Pathway
2
Evaluating MWCNTs for Bladder Cancer
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MWCNTs (diameter 20–50 nm, length 10–30 µm, purity >95%) were purchased from Nanotech Port (Shenzhen, China). Reagents were acquired: sulfuric acid, nitric acid, ammonium hydroxide, FeCl2⋅4H2O, and FeCl3⋅6H2O (Analytical Reagent) from Sinopharm Chemical Reagent (Shanghai, China); antibodies to BCL2, BAX, and Ki67 from Abcam (Cambridge, UK); antibody to cleaved caspase 3 from Cell Signaling Technology (Danvers, MA, USA); EPI from Selleck Chemicals (Houston, TX, USA); a fluorescein isothiocyanate (FITC)–annexin V apoptosis-detection kit from BD Biosciences (San Jose, CA, USA); N-methyl-N-nitrosourea (MNU), DAPI, and FITC-labeled phalloidin from Sigma-Aldrich (St Louis, MO, USA); CCK8 from Dojindo Laboratories (Kumamoto, Japan); and a Cell Light ethynyl deoxyuridine (EdU) Apollo 567 in vitro imaging kit from RiboBio (Guangzhou, China). The human bladder transitional-cell carcinoma 5637 and T24 cell lines were kindly provided by the Stem Cell Bank, Chinese Academy of Sciences in 2016. Eight-week-old female Wistar rats were obtained from the Experimental Animal Center of Shandong University. Animal care and protocols were approved by the Institutional Animal Care and Use Committee of Shandong University. All animal experiments were performed in adherence with the National Institutes of Health Guide for the Care and Use of Laboratory Animals.
3
BrdU Labeling of Thymic Cells
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BrdU (1 mg in 100 μl) was injected by the intraperitoneal (ip) route into pregnant females and E17.5 embryos were harvested after 90 minute chase. Alternatively, newborn pups were ip injected with BrdU (200 μg in 20 μl) and thymic lobes were harvested after 2 hrs. Thymi were digested with 0.5 WU/ml Liberase TM (Roche) containing 0.1% DNASE (Roche). Cells were stained with antibodies to surface proteins, followed by fixation and permeablization and staining with anti-BrdU (BD PharmingenTM BrdU Flow Kit). To determine the frequency of TEC apoptosis, 4% PFA fixed frozen thymus sections were stained with antibody to cleaved caspase 3 (Cell Signaling Technology) and FOXN1.
4
FFPE Liver Tissue Analysis Protocol
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Formalin-fixed paraffin-embedded (FFPE) livers were sectioned at 4 μm thickness for histopathology and immunohistochemistry. Sections were stained with hematoxylin and eosin (H&E) and scanned using a Leica Biosystems ScanScope AT2 brightfield line scanner within Translational Pathology Laboratory at University of North Carolina at Chapel Hill. Cleaved caspase 3 staining was accomplished using Dako Autostainer (Dako, Carpinteria, CA). Tissue sections were de-paraffinized and rehydrated prior to antigen retrieval in Pascal presser cooker (Dako) for 30 sec at 123°C in Tris-EDTA buffer. Slides were cooled, rinsed and then treated sequentially with endogenous peroxidase blocker and universal block prior to incubation for 2 hr at room temperature with antibody to cleaved caspase 3 (Cell Signaling, 9661S) diluted 1:100, and 30 min at room temperature with secondary antibody followed by development with 3,3′-diaminobenzidine and counterstain with hematoxylin. Slides were then dehydrated and mounted. Antibodies used in this study are listed in Supporting Information (S2 Table )
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