Log In Sign up
The largest database of trusted experimental protocols

Ab77231

Manufactured by Abcam
Visit Supplier

Ab77231 is a laboratory equipment product. It is designed for use in scientific research and analysis. The core function of this product is to facilitate specific experimental procedures and data collection. No further details on the intended use or application are provided.

Automatically generated - may contain errors

Lab products found in correlation

Ab1791, by Abcam (2 mentions) Sc 899, by Santa Cruz Biotechnology (1 mentions) Adi spa 901, by Enzo Life Sciences (1 mentions) Sc 5286, by Santa Cruz Biotechnology (1 mentions) Mms 126r, by Fortrea (1 mentions) Ab23738, by Abcam (1 mentions) Ab109388, by Abcam (1 mentions) Anti ar, by Merck Group (1 mentions) Anti pias1, by Santa Cruz Biotechnology (1 mentions) Anti ar, by Santa Cruz Biotechnology (1 mentions) Magna chip protein a magnetic beads, by Merck Group (1 mentions) Anti p21, by Merck Group (1 mentions) Anti tubulin, by Santa Cruz Biotechnology (1 mentions) Normal rabbit igg, by Santa Cruz Biotechnology (1 mentions) Duolink in situ pla kit, by Merck Group (1 mentions) Axr confocal microscope, by Nikon (1 mentions) Ab3742, by Abcam (1 mentions) Ubiquitin, by Cell Signaling Technology (1 mentions) Ab6556, by Abcam (1 mentions) Mab1501, by Merck Group (1 mentions)

5 protocols using ab77231

1

Antibody-based Immunoblotting and ChIP-seq

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies used for immunoblotting were anti-SUMO2/3 (M114-3, MBL), anti-UBC9 (sc10759, Santa Cruz Biotechnology), anti-PIAS1 (ab77231, Abcam), anti-tubulin (sc5286, Santa Cruz Biotechnology), anti-laminB1 (sc6216, Santa Cruz Biotechnology), anti-Pol2 (sc-899, Santa Cruz Biotechnology), anti-histone H3 (ab1791, Abcam), and anti-HSF1 (ADI-SPA-901; ENZO Life Sciences). Antibodies used for ChIP-seq were anti-SUMO2/3 (M114-3, MBL), anti-PIAS1 (ab77231, Abcam), and anti-Pol2 (MMS-126R, Covance).
Niskanen E.A., Malinen M., Sutinen P., Toropainen S., Paakinaho V., Vihervaara A., Joutsen J., Kaikkonen M.U., Sistonen L, & Palvimo J.J. (2015). Global SUMOylation on active chromatin is an acute heat stress response restricting transcription. Genome Biology, 16(1), 153.
+ Open protocol
+ Expand
2

Immunoblotting and Immunoprecipitation of PIAS1, AR, and FOXA1

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
VCaP cell samples were prepared as described (27 (link)) and analyzed by immunoblotting with anti-PIAS1 (Santa-Cruz Biotechnology, sc-8152), anti-AR (28 (link)), anti-p21 (Millipore, OP64) and anti-tubulin (Santa-Cruz Biotechnology, sc-5286) antibodies. The appropriate secondary antibody was from Invitrogen and chemiluminescence detection reagents from Pierce. PIAS1 was immunoprecipitated with anti-PIAS1 antibody (Abcam, ab109388) which was coupled to Magna ChIP™ Protein A Magnetic Beads (Millipore). Normal rabbit IgG (Santa-Cruz Biotechnology, sc-2027) was used as a control antibody and the immunoprecipitates were immunoblotted with anti-AR (Santa-Cruz Biotechnology, sc-7305) antibody. For FOXA1 and PIAS1 immunoprecipitation, anti-FOXA1 (Abcam, ab23738) antibody was used to immunoprecipitate FOXA1 and the immunoprecipitates were immunoblotted with anti-PIAS1 (Abcam, ab77231) antibody.
Toropainen S., Malinen M., Kaikkonen S., Rytinki M., Jääskeläinen T., Sahu B., Jänne O.A, & Palvimo J.J. (2014). SUMO ligase PIAS1 functions as a target gene selective androgen receptor coregulator on prostate cancer cell chromatin. Nucleic Acids Research, 43(2), 848-861.
+ Open protocol
+ Expand
3

PLA Assay for YTHDF2-PIAS1 Interaction

Check if the same lab product or an alternative is used in the 5 most similar protocols
Akata (EBV+) cells were fixed with cold methanol for 5 min at −20°C followed by permeabilization with 0.5% Triton X-100 for 5 min. The PLA experiments were performed using Duolink in situ PLA kit (DUO92101, Sigma) according to manufacturer’s instructions. Briefly, cells were blocked with 3% BSA in PBS at room temperature for 1 h, then incubated with PBS control or a mixture of mouse anti-YTHDF2 (H00051441-B01P, Abnova) and rabbit anti-PIAS1 (ab77231, Abcam) antibodies (1:500 dilution in PBS) at 4°C overnight. Then the probes were incubated at 37°C for 1 h, followed by ligation and amplification. Cell nuclei were stained using Duolink in situ mounting media with 4′,6-diamidino-2-phenylindole and visualized by Nikon AXR confocal microscope.
Sugiokto F.G., Saiada F., Zhang K, & Li R. (2024). SUMOylation of the m6A reader YTHDF2 by PIAS1 promotes viral RNA decay to restrict EBV replication. mBio, 15(2), e03168-23.
+ Open protocol
+ Expand
4

Antibodies Used in Protein Study

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies to human proteins used in this study included: actin (1:20,000 dilution (MAB1501, Millipore, RRID: AB_2223041)); DNMT1 (1:1,000, described in ref. 11); FLAG (1:1,000 (A00187, GenScript, RRID: AB_1720813)); GFP (1:5,000, Abcam (ab6556, RRID: AB_305564)); HA (1:1,000 (11867423001, Roche, RRID: AB_390918)); histone H3 (1:20,000, Abcam (ab1791, RRID: AB_302613)); PARP1 (1:1,000, Santa Cruz Biotechnology (sc-8007, RRID: AB_628105)); PIAS1 (1:1,000 (ab77231, Abcam, RRID: AB_1524188)); RNF4 (1:5,000, described in ref. 54); SUMO1 (1:1,000, Thermo Fisher Scientific (33-2400, RRID: AB_2533109)); SUMO2/3 (1:1,000, Abcam (ab3742, RRID: AB_304041); 1:1,000, Abcam (ab81371, RRID: AB_1658424)); TOP1 (1:500, Bethyl (A302-590A, RRID: AB_2034875)); TOPORS (1:250, sheep polyclonal raised against full-length human TOPORS); ubiquitin (1:1,000, Santa Cruz Biotechnology (sc-8017 AC, RRID: AB_2762364); 1:1,000, Millipore (04-263, RRID: AB_612093)); ubiquitin (K48-linked) (1:1,000, Millipore (05-1307, RRID: AB_1587578)); ubiquitin (K63-linked) (1:1,000, (05-1308, RRID: AB_1587580)); UBE2K (1:1,000, Cell Signaling Technology (3847, RRID: AB_2210768)); and USP7 (1:1,000, Bethyl (A300-033A, RRID: AB_203276)).
Liu J.C.Y., Ackermann L., Hoffmann S., Gál Z., Hendriks I.A., Jain C., Morlot L., Tatham M.H., McLelland G.L., Hay R.T., Nielsen M.L., Brummelkamp T., Haahr P, & Mailand N. (2024). Concerted SUMO-targeted ubiquitin ligase activities of TOPORS and RNF4 are essential for stress management and cell proliferation. Nature structural & molecular biology.
+ Open protocol
+ Expand
5

Protein Expression Analysis in SCLC

Check if the same lab product or an alternative is used in the 5 most similar protocols
NCI-H82 cells were infected with miR-375-expressing (H82-miR-375) or empty (H82-NC) lentivirus at a MOI of 80. The infection efficiency was about 100% as assessed by microscopy of GFP fluorescence. The cells were harvested after 72 hours. Total protein of the cells or fresh tissues (2 AC, 2 SQ, 3 SCLC and 1adjacent normal tissue) were prepared using RIPA lysis buffer. Cell protein lysates were separated in 10% SDS-polyacrylamide gels, electrophoretically transferred to polyvinylidenedifluoride membranes (Roche, Pleasanton, CA), then detected with antibodies including anti-ITPKB (for cell line: ab171984, Abcam; for tissue: NBP1- 81589, Novus Biologicals), anti-RUNX1(ab54869, Abcam), anti-LRP5 (ab38311, Abcam), anti-PIAS1(ab77231, Abcam), anti-FZD8 (ab155650, Abcam), anti-ITGA10 (ab118099, Abcam), anti-GAPDH (Santa Cruz) and peroxidise-conjugated secondary antibodies (Santa Cruz). The intensity of protein fragments was quantified using Quantity One software and was normalized by GAPDH.
Jin Y., Liu Y., Zhang J., Huang W., Jiang H., Hou Y., Xu C., Zhai C., Gao X., Wang S., Wu Y., Zhu H, & Lu S. (2015). The Expression of miR-375 Is Associated with Carcinogenesis in Three Subtypes of Lung Cancer. PLoS ONE, 10(12), e0144187.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!