The oral glucose tolerance test (OGTT) was performed at the end of week 0 and 6 (end of the experiment), according to Ferrere et al. (2016 (link)), with some modifications. A solution of 0·2% d ‐glucose was given to each animal via gavage after overnight (12 h) fasting conditions, then blood was collected from the tail after 0, 30, 60 and 120 min. Glucose concentration in the blood serum was measured by putting a blood drop on a Comfort Curve Strips (F. Hoffmann–La Roche, Basel, Switzerland) that was then inserted into an ACCU‐CHEK Advantage Glucometer (Roche, Basel, Switzerland) and the OGT was determined according to Cardoso et al. (2011 (link)).
Accu chek advantage glucometer
Manufactured by Roche
Sourced in Switzerland, United States, Germany, Canada
The Accu-Chek Advantage glucometer is a compact and portable device designed for blood glucose monitoring. It provides users with accurate and reliable blood glucose readings to support diabetes management.
Automatically generated - may contain errors
10 protocols using accu chek advantage glucometer
1
Oral Glucose Tolerance Test Protocol
2
Glucose and Insulin Tolerance Tests
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
GTTs were performed in 12-h-fasted fl/fl and CD36LKO mice following an intraperitoneal injection of glucose (1 g/kg body weight), and ITTs were performed in 4-h-fasted fl/fl and CD36LKO mice following an intraperitoneal injection of insulin (0.7 units/kg body weight). Blood glucose levels from tail vein blood were measured at 0, 15, 30, 60, and 120 min with an ACCU-CHEK Advantage glucometer (Roche Diagnostics, USA).
3
Serum Lipid and Glucose Measurement
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serum was separated by centrifuging venous blood from mice at 3000 rpm for 15 minutes, and the total cholesterol (TC) and triglyceride (TG) levels were measured using assay kits (Dong'ou, Wenzhou, China) according to the manufacturer's instructions. Glucose was measured in peripheral blood with an ACCU-CHEK Advantage glucometer (Roche Diagnostics Inc., Penzberg, Germany). Each sample was tested three times.
4
High-fat diet impacts metabolic profile
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Experiments were conducted in accordance with the Guide for the Care and Use of Laboratory Animals, National Health and Medical Research Council/Commonwealth Scientific, and Industrial Research Organization/ACC Australian Code of Practice for the Care and Use of Animals for Scientific Purposes (1997), as approved (University of Melbourne Animal Ethics Committee). 8-wk-old male Sprague-Dawley rats were fed either a control (Specialty Feeds, SF13-081; 12% lipid energy intake) or high-fat diet (HFD; SF04-001; 43% lipid intake) for 15–16 wk. Food and water were available ad libitum, and intake and body weights were monitored weekly. At the completion of the feeding period, rats were anesthetized with isoflurane and decapitated. Trunk blood was collected for measurement of plasma glucose (ACCU-CHEK Advantage glucometer; Roche Diagnostics) and lipids (cholesterol, triglycerides, and high- and low-density lipoproteins; Cobas B 101; Roche Diagnostics).
5
Glucose and Insulin Tolerance Tests
6
Glucose Tolerance Test in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Glucose tolerance test (GTT) was performed as previously described 36 (link). Mice were overnight fasted and then injected with glucose (1 g/kg b.wt), intraperitoneally. Following injection, glucose levels were detected in blood obtained from the tail tip of mice at the interval of 15, 30, 60, and 120 min using an ACCU-CHEK Advantage Glucometer (Roche Diagnostics, Laval, QC, Canada).
7
Glucose and Insulin Tolerance Tests in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
On GD15, mice were fasted 6 h and injected intraperitoneally with 2.0 g/kg glucose for the glucose tolerance test. Then, blood glucose concentrations were analyzed using an ACCU-CHEK advantage glucometer (Roche Diagnostics). The glucose tolerance tests were recorded at 0, 30, 60, 90, and 120 min after glucose injection. For the insulin tolerance tests, mice were intraperitoneally injected with insulin (1.0 mU/kg) after a 60-min fast and the blood glucose concentrations were measured at baseline and after insulin injection (30, 60, 90, and 120 min). The homeostasis model assessment for insulin resistance (HOMA-IR) was calculated by (fasting blood glucose [mmol/l]*fasting plasma insulin [µU/ml])/22.5 as described previously15 (link). Quantitative Insulin Sensitivity Check Index (QUICKI) was calculated by 1/(log fasting blood glucose [mmol/l] + log fasting plasma insulin [µU/ml]) as described previously16 (link).
8
Glucose and Insulin Tolerance Tests
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Glucose and insulin tolerance tests were performed on mice that were fasted for 4 hours. Insulin (0.75 U/kg) or glucose (2 g/kg) were intraperitoneally injected into mice and glucose concentrations were determined with an Accu-Chek Advantage glucometer (Roche) in blood collected from the tail vein at the indicated time points.
9
Insulin, GLP-1, and Intralipid Interaction
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Insulin, GLP-1, and Intralipid + heparin were infused for various times as detailed in each study protocol. Insulin (Humulin; Eli Lilly and Company) was infused systemically at 3 mU/kg/min with arterial blood glucose determined every 10 min using an Accu-Chek Advantage glucometer (Roche Diagnostics, Indianapolis, IN) and 30% dextrose (30% weight for volume) infused at a variable rate to maintain blood glucose within 10% of basal. The time course and area under the curve (AUC) of the glucose infusion rate (GIR; mg/kg/min) were calculated. GLP-1 (7–36) amide (Bachem Americas, Inc.) was infused continuously at 30 pmol/kg/min. Intralipid (6.6%) plus heparin (60 U/mL) was infused at 5 µl/min. At the rates selected, insulin and GLP-1 each potently recruit muscle microvasculature (8 (link),11 (link),23 (link),24 (link)), and Intralipid + heparin abrogates insulin-mediated muscle microvascular and metabolic responses (8 (link),33 (link)).
10
Intraperitoneal Glucose Tolerance Test
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For glucose tolerance tests (GTT), mice were subjected to an overnight fast (∼16 h) and intraperitoneal (IP) injection of D-glucose (1 g/kg of body weight). Blood glucose was monitored at 0, 15, 30, 60, and 90 min after glucose injection using a portable glucose analyzer (Accu-Chek Advantage glucometer, Roche, Mannheim, Germany).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!