All chemicals were purchased from Sigma-Aldrich Chemical Co. or Thermo Fisher Scientific. EB-NH2 was synthesized as described previously 26 (link), 27 (link). Cyclic-(Arg-Gly-Asp-D-Phe-Lys) (RGD) peptide was purchased from C.S. Bio. InvivoPlus anti-mouse PD-L1 mAb was purchased from BioXCell (Cat. No. BP0101). The murine colon adenocarcinoma cell line, MC38, was purchased from the China National Infrastructure of Cell Line Resource. Labeling efficiency and radiochemical purity were tested using a Mini-Scan radio-TLC Scanner (BioScan, USA) and Dionex Ulti-Mate 3000 high performance liquid chromatography (HPLC; Thermo Scientific, USA), with a flow-counter radioactivity detector (BioScan, USA). Radioactivity was measured using a γ-counter (WIZARD 2480; Perkin-Elmer, USA) and CRC-25R Dose Calibrators (CAPIN-TEC Inc., USA). SPECT imaging was performed using a nanoScan SPECT/CT scanner (Mediso, Hungary).
Mini scan radio tlc scanner
Manufactured by Bioscan
Sourced in United States
The Mini-Scan radio-TLC Scanner is a compact device designed for the detection and analysis of radioactive samples on thin-layer chromatography (TLC) plates. It provides precise and accurate quantification of radioactive compounds, enabling researchers to study and analyze their samples effectively.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using mini scan radio tlc scanner
1
Radiolabeled Anti-PD-L1 Antibody Protocol
2
Radiolabeling and Characterization of 99mTc-GlcNAc-PEI
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Briefly, 100 μg/100 μL of GlcNAc-PEI and 20 μL of SnCl2 (2 mg/mL in 0.1 M HCl) were mixed in a vial. Next, 185~370 MBq of freshly Na99mTcO4 obtained from Zhongshan Hospital affiliated with Xiamen University was added to the mixture. The reaction mixture was then allowed to incubate at room temperature for 30 min to obtain the resulting radiotracer 99mTc-GlcNAc-PEI. The labeling efficiency of 99mTc-GlcNAc-PEI was determined by instant thin-layer chromatography (ITLC). The chromatography analyses were performed on ITLC silica gel strips (Pall Life Sciences, Ann Arbor, MI, USA) with acid-citrate-dextrose buffer (0.068 M citrate, 0.074 M dextrose, pH 5.0) as a mobile phase. The stability of the radiotracer was tested in the same way after incubation in saline or mice serum at 37 ºC for 4 h. Next, all the strips were detected using a Mini-Scan radio-TLC Scanner (BioScan, Poway, CA, USA).
3
Multimodal Imaging Tracer Protocols
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Na131I and Na99mTcO4 were obtained from Zhongshan Hospital Affiliated of Xiamen University. The 18F-FDG was obtained from the First Affiliated Hospital of Xiamen University. Na125I was obtained from China Isotope & Radiation Corporation. GSA kits were obtained from Beijing Shihong Pharmaceutical Center of Beijing Normal University. Palladium(ii ) acetylacetonate (Pd(acac)2, 99%) was bought from Alfa Aesar. Poly(vinylpyrrolidone) (PVP K30) was obtained from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). Methoxypoly(ethylene glycol) thiol (mPEG-SH, 5 K) was bought from Sinopeg Biotech Co., Ltd. N,N-Dimethylpropionamide (DMP) was obtained from Sigma-Aldrich Co., LLC. The TLC strips were detected with a Mini-Scan radio-TLC Scanner (BioScan, USA). The radioactivity was measured with a γ-counter (WIZARD 2480, Perkin-Elmer, USA) and CRC-25R Dose Calibrators (CAPIN-TEC. Inc, USA). The SPECT imaging study was performed with a nanoScan SPECT/CT scanner (Mediso, HUNGARY). Animal PET/CT scans were performed using an Inveon device (Siemens Medical Solutions Inc., USA). PA imaging was performed with Nexus 128 photoacoustic tomography systems (Ann Arbor, MI, USA). TEM images were recorded on a TECNAI F-30 high-resolution transmission electron microscope operating at 300 kV.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!