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Concanavalin a (cona)

Manufactured by Corning
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ConA is a lectin protein derived from the jack bean plant. It has the ability to agglutinate various cells, including red blood cells, and can be used to precipitate and isolate glycoproteins. ConA is commonly used in laboratory settings for applications involving cell biology, biochemistry, and immunology.

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Lab products found in correlation

Andpenicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Pe conjugated anti human il 2rα, by BioLegend (2 mentions) Accuri c6 flow cytometer, by BD (2 mentions)

3 protocols using concanavalin a (cona)

1

Purification and Culture of IL-2Rα+ YT-1 NK Cells

Cited 1 time
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Unmodified YT-153 and
IL-2Rα+ YT-1 human NK cells54 were cultured in RPMI complete medium
(RPMI 1640 medium supplemented with 10% fetal bovine serum, 2 mM L-glutamine,
minimum non-essential amino acids, sodium pyruvate, 25 mM HEPES, and
penicillin-streptomycin [Gibco]). CTLL-2 cells purchased from ATCC were cultured
in RPMI complete with 10% T-STIM culture supplement with ConA (Corning). 24
hours prior to signaling studies, CTLL-2 cells were resuspended in RPMI lacking
T-STIM culture supplement for IL-2 starvation. Viability (>95%) of CTLL-2
cells was verified by trypan blue exclusion (counted in a hemocytometer)
immediately before performing the signaling assays. All cells were maintained at
37°C in a humidified atmosphere with 5% CO2. The subpopulation
of YT-1 cells expressing IL-2Rα was purified via magnetic selection as
described previously39 (link).
Enrichment and persistence of IL-2Rα expression were monitored by
analysis of PE-conjugated anti-human IL-2Rα (Biolegend) antibody binding
on an Accuri C6 flow cytometer (BD Biosciences).
Silva D.A., Yu S., Ulge U., Spangler J.B., Jude K.M., Labão-Almeida C., Ali L.R., Quijano-Rubio A., Ruterbusch M., Leung I., Biary T., Crowley S.J., Marcos E., Walkey C.D., Weitzner B.D., Pardo-Avila F., Castellanos J., Carter L., Stewart L., Riddell S., Pepper M., Bernardes G.J., Dougan M., Garcia K.C, & Baker D. (2019). De novo design of potent and selective mimics of IL-2 and IL-15. Nature, 565(7738), 186-191.
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2

Purification and Culture of IL-2Rα+ YT-1 NK Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Unmodified YT-153 and
IL-2Rα+ YT-1 human NK cells54 were cultured in RPMI complete medium
(RPMI 1640 medium supplemented with 10% fetal bovine serum, 2 mM L-glutamine,
minimum non-essential amino acids, sodium pyruvate, 25 mM HEPES, and
penicillin-streptomycin [Gibco]). CTLL-2 cells purchased from ATCC were cultured
in RPMI complete with 10% T-STIM culture supplement with ConA (Corning). 24
hours prior to signaling studies, CTLL-2 cells were resuspended in RPMI lacking
T-STIM culture supplement for IL-2 starvation. Viability (>95%) of CTLL-2
cells was verified by trypan blue exclusion (counted in a hemocytometer)
immediately before performing the signaling assays. All cells were maintained at
37°C in a humidified atmosphere with 5% CO2. The subpopulation
of YT-1 cells expressing IL-2Rα was purified via magnetic selection as
described previously39 (link).
Enrichment and persistence of IL-2Rα expression were monitored by
analysis of PE-conjugated anti-human IL-2Rα (Biolegend) antibody binding
on an Accuri C6 flow cytometer (BD Biosciences).
Silva D.A., Yu S., Ulge U., Spangler J.B., Jude K.M., Labão-Almeida C., Ali L.R., Quijano-Rubio A., Ruterbusch M., Leung I., Biary T., Crowley S.J., Marcos E., Walkey C.D., Weitzner B.D., Pardo-Avila F., Castellanos J., Carter L., Stewart L., Riddell S., Pepper M., Bernardes G.J., Dougan M., Garcia K.C, & Baker D. (2019). De novo design of potent and selective mimics of IL-2 and IL-15. Nature, 565(7738), 186-191.
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3

Cell Culture Protocols for CTLL-2 and B16-F10

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All cell lines used in this study were obtained from ATCC and cultured according to the provided protocols. The complete culture medium of CTLL-2 cell line was formulated as 10% FBS, an additional 2 mM L-glutamine, and 1 mM sodium pyruvate in ATCC-formulated RPMI-1640 containing 10% T-STIM with Con A (Corning). The complete medium of B16-F10 cell line is formulated as 10% FBS in ATCC-formulated DMEM.
Shen Z., Xiang Y., Vergara S., Chen A., Xiao Z., Santiago U., Jin C., Sang Z., Luo J., Chen K., Schneidman-Duhovny D., Camacho C., Calero G., Hu B, & Shi Y. (2021). A resource of high-quality and versatile nanobodies for drug delivery. iScience, 24(9), 103014.
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