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Sc 423402 act

Manufactured by Santa Cruz Biotechnology
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Sourced in United States

Sc-423402-ACT is a laboratory instrument designed for the detection and analysis of specific biomolecules. It utilizes advanced spectroscopic techniques to provide accurate and reliable data for research applications. The core function of this product is to facilitate the identification and quantification of target analytes in complex samples.

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Lab products found in correlation

Pci neo vector, by Promega (2 mentions) Dcas9 vp64 p65 rta construct19, by Addgene (2 mentions) Pci neo expression vector, by Promega (2 mentions)

2 protocols using sc 423402 act

1

Synthetic Transcriptional Activators for CRISPR Modulation

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
VP64 and VP160 sequences containing 4 or 10 copies of the nucleotides encoding VP16 minimal transactivator sequence DALDDFDLDML18 (link) (Table S3) were synthesized (GenScript) and subcloned in the N-terminus of dCas9 in the pCI-neo vector (Promega). The dCas9. VP64-p65-Rta (VPR) construct19 (link) which served as the template for the various PCR amplifications was purchased from Addgene (#63,798; Cambridge, MA, USA). All VP-based constructs were subcloned into the pCI/neo expression vector (Promega) for uniformity. The Cas-effector with synthetic activation motif (SAM) targeting the mouse TIMP1 gene was purchased from Santa Cruz Biotechnology (sc-423402-ACT, Santa Cruz, CA, USA).
Duellman T., Doll A., Chen X., Wakamiya R, & Yang J. (2017). dCas9-mediated transcriptional activation of tissue inhibitor of metalloproteinases. Metalloproteinases in medicine, 4, 63-73.
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2

Synthetic Transcriptional Activators for CRISPR Modulation

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
VP64 and VP160 sequences containing 4 or 10 copies of the nucleotides encoding VP16 minimal transactivator sequence DALDDFDLDML18 (link) (Table S3) were synthesized (GenScript) and subcloned in the N-terminus of dCas9 in the pCI-neo vector (Promega). The dCas9. VP64-p65-Rta (VPR) construct19 (link) which served as the template for the various PCR amplifications was purchased from Addgene (#63,798; Cambridge, MA, USA). All VP-based constructs were subcloned into the pCI/neo expression vector (Promega) for uniformity. The Cas-effector with synthetic activation motif (SAM) targeting the mouse TIMP1 gene was purchased from Santa Cruz Biotechnology (sc-423402-ACT, Santa Cruz, CA, USA).
Duellman T., Doll A., Chen X., Wakamiya R, & Yang J. (2017). dCas9-mediated transcriptional activation of tissue inhibitor of metalloproteinases. Metalloproteinases in medicine, 4, 63-73.
+ Open protocol
+ Expand

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