Quick seal polyallomer tubes

Manufactured by Beckman Coulter

Sourced in United States

Quick-Seal polyallomer tubes are laboratory centrifugation tubes designed to withstand high-speed centrifugation. They are made of a polyallomer material, which provides durability and resistance to solvents and chemicals. The tubes are available in various sizes to accommodate different sample volumes.

Automatically generated - may contain errors

Lab products found in correlation

Nd 2000 uv vis spectrophotometer, by Thermo Fisher Scientific (3 mentions) Fraction recovery system, by Beckman Coulter (3 mentions) Optima l 100 xp, by Beckman Coulter (2 mentions) Optima xe 100, by Beckman Coulter (1 mentions) Optiprep, by Merck Group (1 mentions) Powersoil dna extraction kit, by Qiagen (1 mentions) Vti 65.2 rotor, by Beckman Coulter (1 mentions) Mobio powermax dna isolation kit, by Qiagen (1 mentions) Nd 1000 spectrophotometer, by Thermo Fisher Scientific (1 mentions) Beckman optima xpn 100, by Beckman Coulter (1 mentions) Ar200, by Leica (1 mentions) Beckman ultracentrifuge, by Beckman Coulter (1 mentions) Mobio powersoil dna isolation kit, by Qiagen (1 mentions)

Spelling variants of this product

Polyallomer tubes (33 citations) Quick‐Seal tube (2 citations)

7 protocols using quick seal polyallomer tubes

Isolation and Purification of Extracellular Vesicles

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell
supernatant was transferred to 94 mL quick-seal polyallomer tubes
(Beckman Coulter) and centrifuged at 20,000g_avg for 25 min at 4 °C using a 45Ti rotor and an Optima
XE-100 ultracentrifuge (Beckman Coulter) to pellet intermediate-size
EVs. The supernatant was carefully removed, transferred to new tubes,
and ultracentrifuged at 100,000g for 120 min at 4
°C (Type 45 Ti, k-factor 210.4) to pellet small EVs (also known
as exosomes). The exosome-like pellet was resuspended in a total of
1 mL of PBS and floated in a high-resolution iodixanol density gradient
(Optiprep, Sigma-Aldrich) at 120,000g_avg for 16 h at 4 °C (SW 32.1 Ti, k-factor 249.1, Beckman Coulter)
as previously described by our group.^{31 (link)} Nine fractions were collected from top to bottom (corresponding
to iodixanol concentrations of 10–50%). After full characterization
of each fraction, F1–F3 (corresponding to 10, 20, and 22% iodixanol)
were pooled, transferred to new 94 mL PBS tubes, and ultracentrifuged
at 120,000g_avg for 2.5 h (Type 45 Ti,
k-factor 175.3). Those EV pellets were resuspended in PBS and stored
at −80 °C.

Lázaro-Ibáñez E., Faruqu F.N., Saleh A.F., Silva A.M., Tzu-Wen Wang J., Rak J., Al-Jamal K.T, & Dekker N. (2021). Selection of Fluorescent, Bioluminescent, and Radioactive Tracers to Accurately Reflect Extracellular Vesicle Biodistribution in Vivo. ACS Nano, 15(2), 3212-3227.

+ Open protocol

+ Expand

Isopycnic Density Gradient Centrifugation for Ammonia Oxidizer Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Isopycnic density gradient centrifugation was performed on DNA extracted from each 30-day microcosm sample as previously described (22 (link), 37 (link), 86 (link)). Briefly, 1 μg DNA was mixed in 8.5 ml CsCl solution. The mixture was adjusted to a final CsCl buoyant density of 1.71 g ml⁻¹ and then transferred to 8-ml quick-seal polyallomer tubes (Beckman Coulter, Palo Alto, CA, USA) before centrifugation in a MLN80 rotor (Beckman Coulter) was performed at 45,000 rpm for 60 h at 20°C. Each tube was divided into 15 fractions (500 μl each), polyethylene glycol was used to precipitate DNA, followed by 70% ethanol purification, and the resultant DNA pellet was dissolved in 30 μl sterile water. AOA, AOB, and comammox amoA gene abundances were then determined in each DNA fraction (fractions 2 to 14) by qPCR as described above. Autotrophic growth of ammonia oxidizer communities was determined by comparing [¹²C]CO₂ and [¹³C]CO₂ incorporation profiles, i.e., when the buoyant density peaks were distinct between the two treatments.

Zhao J., Meng Y., Drewer J., Skiba U.M., Prosser J.I, & Gubry-Rangin C. (2020). Differential Ecosystem Function Stability of Ammonia-Oxidizing Archaea and Bacteria following Short-Term Environmental Perturbation. mSystems, 5(3), e00309-20.

+ Open protocol

+ Expand

Soil Metagenome Extraction and Density Gradient Centrifugation

Check if the same lab product or an alternative is used in the 5 most similar protocols

DNA extraction was conducted on two soil samples from each treatment that were incubated for 3, 6, and 9 days, using a Powersoil DNA extraction kit (MO BIO Laboratories, Inc. Carlsbad, CA, USA) following the manufacturer’s instructions. The DNA content was quantified with an ND-2,000 UV-Vis spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). Thereafter, about 10,000-ng DNA was added to Quick-Seal polyallomer tubes (13 × 51 mm, 5.1 mL, Beckman Coulter, Pasadena, CA, USA) and spun in Tris-EDTA (TE, pH 8.0)/CsCl solution. Prior to sealing the tubes with one cordless quick-seal tube topper (Beckman Coulter), the average buoyant density (BD) of all prepared gradients was confirmed with a digital refractometer (model AR200, Leica Microsystems Inc., Buffalo Grove, IL, USA), and adjusted by adding small volumes of CsCl solution or Tris-EDTA buffer. The tubes were transferred to an ultracentrifuge (Optima L-100XP, Beckman Coulter). Centrifugation was performed at 45,000 g (20°C) for 48 h. Subsequently, the centrifuge tubes were placed onto a fraction recovery system (Beckman Coulter) and fractions (150 μL for each) were collected. The BD of each fraction was then measured, and CsCl was removed by introducing concentrated ethanol [30 (link)].

Jiang L., Song M., Luo C., Zhang D, & Zhang G. (2015). Novel Phenanthrene-Degrading Bacteria Identified by DNA-Stable Isotope Probing. PLoS ONE, 10(6), e0130846.

+ Open protocol

+ Expand

CsCl Gradient Centrifugation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

A density gradient centrifugation was done with CsCl gradients following the protocol of Dunford and Neufeld (2010). Briefly, 4 μg of DNA from each sample was added to a CsCl solution to the final density of 1.725 g ml⁻¹ in 5.1 ml QuickSeal Polyallomer tubes (Beckman Coulter, Brea, CA). The samples were centrifuged for 60 h at 44 000 rpm (177 000 g_av) at 20°C using a Vti 65.2 rotor (Beckman Coulter). The samples were then fractionated to 13 equal fractions, and the gradient formation was checked by measuring the density of each fraction from 10 μl of sample with a digital refractometer (AR2000 Reichert Technologies, Buffalo, NY). DNA was precipitated by adding 2 volumes of PEG solution (30% PEG6000, 1.6 M NaCl) and 20 μg of polyacrylamide as a carrier, incubating at RT for 2 h and pelleting the DNA by centrifugation at 13 000 ×rcf for 30 min at 4°C. Pellets were washed with 70% ethanol, let dry and resuspended in PCR‐grade water.

Suominen S., Dombrowski N., Sinninghe Damsté J.S, & Villanueva L. (2020). A diverse uncultivated microbial community is responsible for organic matter degradation in the Black Sea sulphidic zone. Environmental Microbiology, 23(6), 2709-2728.

+ Open protocol

+ Expand

Density Gradient DNA Fractionation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total DNA from the matrices were extracted using the Mobio PowerMax DNA Isolation Kit (MO BIO Laboratories, Inc., Carlsbad, CA) according to the manufacturer's instructions. DNA concentration was determined using a ND-1000 spectrophotometer (Nanodrop Technologies-ThermoFisher Scientific, Wilmington, DE). Up to 10, 000 ng DNA, in Tris-EDTA (10 mM Tris-HCl, 1 mM EDTA, pH 8.0)-cesium chloride (CsCl) solution, was put into Quick-Seal polyallomer tubes (13 mm × 51 mm, 5.1 mL; Beckman Coulter), adjusted to a buoyant density (BD) of ~1.77 g mL -1 , sealed and centrifuged at 178, 000 × g (20 o C), for 48 h using an ultracentrifuge (Beckman Optima XPN-100, Beckman Coulter, USA) (Song et al., 2015) (link). Then the centrifuged DNA was fractioned into 22 fractions. Each fraction of the DNA was purified using the Omega MicroElute DNA Clean-Up Kit (Omega Bio-Tek, Norcross, GA, USA).

Xu H.J., Bai J., Li W., Murrell J.C., Zhang Y., Wang J., Luo C, & Li Y. (2021). Mechanisms of the enhanced DDT removal from soils by earthworms: Identification of DDT degraders in drilosphere and non-drilosphere matrices. Journal of hazardous materials, 404(Pt B).

+ Open protocol

+ Expand

Density Gradient Centrifugation of Microbial DNA

Check if the same lab product or an alternative is used in the 5 most similar protocols

After centrifuging 100 mL of each water sample from the 12 C-PHE and 13 C-PHE treatments, total nucleic acids were extracted from the resulting cell pellets using the PowerSoil DNA Isolation Kit (MO BIO, Carlsbad, CA, USA) according to the manufacturer's instructions. 39 DNA content was quantified using the ND-2,000 UV-Vis spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA).
Approximately 5 µg DNA were added to Quick-Seal polyallomer tubes (13 × 51 mm, 5.1 mL; Beckman Coulter, Pasadena, CA, USA) and mixed with Tris-EDTA (pH 8.0)-CsCl solution at a final buoyant density (BD) of ~1.77 g/mL. The BD was determined using a digital refractometer (model AR200; Leica Microsystems Inc., Buffalo Grove, IL, USA). After balancing and sealing, the tubes were transferred to an ultracentrifuge (Optima L-100XP, Beckman Coulter) at 45,000 g (20°C) for 48 h. Subsequently, DNA in the tube was fractioned (400 µL each) and collected using a fraction recovery system (Beckman Coulter). After the BD measurements, the DNA fractions were purified using the method described by Sun et al. 40 The relationships between BD and the fraction number or DNA concentration are listed in Figure S1 and Figure S2, respectively.

Li J., Luo C., Song M., Dai Q., Jiang L., Zhang D, & Zhang G. (2017). Biodegradation of Phenanthrene in Polycyclic Aromatic Hydrocarbon-Contaminated Wastewater Revealed by Coupling Cultivation-Dependent and -Independent Approaches. Environmental science & technology, 51(6).

+ Open protocol

+ Expand

Isopycnic Ultracentrifugation of PAH-Amended DNA

Check if the same lab product or an alternative is used in the 5 most similar protocols

On Day 3, 9 and 14, samples amend with 13 C labeled and unlabeled PAHs (ANT, PHE and FLA) were sacrificed for DNA extraction. Total genomic DNA was extracted using a MoBio power soil DNA isolation kit (MO BIO Laboratories, Inc.
Carlsbad, CA) according to the manufacturer's instruction. DNA concentrations were determined using an ND-2000 UV-Vis spectrophotometer (NanoDrop Technologies, Wilmington, DE). Then DNA was prepared as previously for isopycnic ultracentrifugation and gradient fractionation [32, 33] . Briefly, approximately 10,000-ng DNA was added to Quick-Seal polyallomer tubes (13×51 mm, 5.1 ml, Beckman Coulter), along with a Tris-EDTA (TE, pH 8.0)/CsCl solution with a final buoyant density of ∼1.77 g mL -1 . After sealed with a cordless quick-seal tube topper, the tubes were transferred to a Beckman ultracentrifuge and centrifuged at 178,000 × g (20°C) for 48 h. Following centrifugation, 150-μL fractions were collected from each tube using a fraction recovery system (Beckman). The BD value of each fraction was then measured. CsCl was removed by glycogen-assisted ethanol precipitation [35] .

Song M., Jiang L., Zhang D., Luo C., Wang Y., Yu Z., Yin H, & Zhang G. (2016). Bacteria capable of degrading anthracene, phenanthrene, and fluoranthene as revealed by DNA based stable-isotope probing in a forest soil. Journal of hazardous materials, 308.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!