Log In Sign up
The largest database of trusted experimental protocols

Luminex instrument

Manufactured by Bio-Rad
Visit Supplier
Sourced in United States

The Luminex instrument is a multiplex analytical system that utilizes flow cytometry technology to detect and quantify multiple analytes simultaneously in a single sample. It provides a platform for high-throughput, rapid analysis of various biomolecules, including proteins, nucleic acids, and cell-based assays.

Automatically generated - may contain errors

Lab products found in correlation

Tissuemiser, by Thermo Fisher Scientific (2 mentions) Quantigene 2.0 multiplex kit, by Thermo Fisher Scientific (2 mentions) T per tissue protein extraction reagent, by Thermo Fisher Scientific (2 mentions) Calbiochem protease inhibitor cocktail set 1, by Merck Group (2 mentions) Match it dna software, by Immucor (1 mentions) Quantigene plex magnetic separation assay kit, by Thermo Fisher Scientific (1 mentions) Bead ruptor 24, by Omni International (1 mentions) Quantigene plex 2, by Panomics (1 mentions) Quantigene plex 2, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Luminex IS 100 instrument Luminex 200 instrument system Bio-Plex 200 Luminex instrument Bio-Plex Luminex 200 XYP instrument Luminex IS 200 instrument Luminex Bio-Plex 200 IS 100 instrument Luminex 200 instrument

7 protocols using luminex instrument

1

HLA Genotyping of Pregnancy Cohort

Check if the same lab product or an alternative is used in the 5 most similar protocols
Class I and II HLA genotyping was performed for DNA prepared from infant samples collected in the pregnancy cohort. Commercial kits for HLA‐A, HLA‐B, HLA‐C, DRB1, DRB3,4,5, DQB1 and DPA/B loci (Immucor, Norcross, Georgia, USA) were used for locus‐specific PCR amplification according to manufacturer's instructions, followed by detection via allele‐specific probes using a Luminex instrument (Bioplex, Biorad) and Match‐It DNA software (Immucor). The typing was reported at the 2/4 ‐ digit resolution.
Savulescu D.M., Groome M., Malfeld S.C., Madhi S., Koen A., Jones S., Duxbury V., Scheuermaier K., De Assis Rosa D, & Suchard M. (2020). HLA antibody repertoire in infants suggests selectivity in transplacental crossing. American Journal of Reproductive Immunology, 84(2), e13264.
+ Open protocol
+ Expand
2

Multiplex Cytokine Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Plasma contents of IL-1, IL-6, IL-4, and TNF-α were determined in duplicate by using Bio-Plex Pro cytokine assays kit (Bio-Rad, Nazareth, Belgium) and measured using a Luminex instrument (Bio-Plex; Bio-Rad) following the manufacturer’s instructions.
Everard A., Matamoros S., Geurts L., Delzenne N.M, & Cani P.D. (2014). Saccharomyces boulardii Administration Changes Gut Microbiota and Reduces Hepatic Steatosis, Low-Grade Inflammation, and Fat Mass in Obese and Type 2 Diabetic db/db Mice. mBio, 5(3), e01011-14.
+ Open protocol
+ Expand
3

Dnm1l and actb mRNA Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
For quantification of mRNA levels of Dnm1l and actb in muscle, we used the QuantiGene Plex Magnetic Separation Assay kit (Affymetrix, Santa Clara, CA, USA) following the manufacturer’s instructions and as previously described [32 ]. The signal was detected with a Luminex instrument (Bio-Rad, Milan, Italy). For each sample, the average signal (MFI) for Dnm1l and actb were determined and, after average background signal subtraction, Dnm1l signal was normalized to the housekeeping gene signal.
Vicario N., Spitale F.M., Tibullo D., Giallongo C., Amorini A.M., Scandura G., Spoto G., Saab M.W., D’Aprile S., Alberghina C., Mangione R., Bernstock J.D., Botta C., Gulisano M., Buratti E., Leanza G., Zorec R., Vecchio M., Di Rosa M., Li Volti G., Lazzarino G., Parenti R, & Gulino R. (2021). Clobetasol promotes neuromuscular plasticity in mice after motoneuronal loss via sonic hedgehog signaling, immunomodulation and metabolic rebalancing. Cell Death & Disease, 12(7), 625.
+ Open protocol
+ Expand
4

Quantifying Trabecular Bone mRNA Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
A sample of trabecular bone was taken from the distal right femur and homogenized in buffer provided by the manufacturer using a high-speed homogenizer (Bead Ruptor 24, Omni, Kennesaw, GA, USA) and steel beads at 4°C. The tissue lysate was incubated at 65°C and centrifuged to precipitate the debris.
mRNA expression was quantified using the QuantiGene Plex 2.0® technique (Panomics/Affymetrix Inc, Santa Clara, CA, USA) according to the manufacturer’s instructions. Oligonucleotide probe sets for the genes of interest were designed by the manufacturer. The tissue lysate was pipetted into a 96-well plate preloaded with capture reagent and a probe set. After incubation overnight at 54°C, hybridization with the preamplifier, amplifier, and biotinylated label was carried out. Luminescence was measured using a Luminex® instrument (Bio-Rad, Hercules, CA, USA), and the mean fluorescence intensity specific for each gene (proportional to the mRNA captured by the bead) was generated. Expression of each gene was normalized to the expression of glyceraldehyde 3-phosphate dehydrogenase. The genes of interest are listed in Table 1.
Chin K.Y, & Ima-Nirwana S. (2014). Effects of annatto-derived tocotrienol supplementation on osteoporosis induced by testosterone deficiency in rats. Clinical Interventions in Aging, 9, 1247-1259.
+ Open protocol
+ Expand
5

Multiplex Gene and Protein Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
For gene array, corneas were processed using a magnetic bead-based Quantigene 2.0 multiplex kit (Affymetrix). Briefly, samples were lysed to extract RNA and incubated with specific target probes overnight. Signals were amplified with a hybridization technique, and detected using a luminex instrument (Biorad) after adding streptavidin with phycoerythrin as a substrate. For protein array, tissues were homogenized with a Tissuemiser (Fisher Scientific) in Tissue Protein Extraction Reagent (T-PER, Fisher Scientific) in the presence of 1x Calbiochem protease inhibitor cocktail set I (Millipore). Homogenates were centrifuged in a microcentrifuge at 10,000×g for 90 sec at 4° C, and the supernatants were evaluated for analyte content by multiplex (Millipore) or ELISA (R&D) assays.
Gurung H.R., Carr M.M., Bryant K., Chucair-Elliott A.J, & Carr D.J. (2017). Fibroblast growth factor-2 Drives and Maintains Progressive Corneal Neovascularization following HSV-1 Infection. Mucosal immunology, 11(1), 172-185.
+ Open protocol
+ Expand
6

Multiplex Gene and Protein Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
For gene array, corneas were processed using a magnetic bead-based Quantigene 2.0 multiplex kit (Affymetrix). Briefly, samples were lysed to extract RNA and incubated with specific target probes overnight. Signals were amplified with a hybridization technique, and detected using a luminex instrument (Biorad) after adding streptavidin with phycoerythrin as a substrate. For protein array, tissues were homogenized with a Tissuemiser (Fisher Scientific) in Tissue Protein Extraction Reagent (T-PER, Fisher Scientific) in the presence of 1x Calbiochem protease inhibitor cocktail set I (Millipore). Homogenates were centrifuged in a microcentrifuge at 10,000×g for 90 sec at 4° C, and the supernatants were evaluated for analyte content by multiplex (Millipore) or ELISA (R&D) assays.
Gurung H.R., Carr M.M., Bryant K., Chucair-Elliott A.J, & Carr D.J. (2017). Fibroblast growth factor-2 Drives and Maintains Progressive Corneal Neovascularization following HSV-1 Infection. Mucosal immunology, 11(1), 172-185.
+ Open protocol
+ Expand
7

Quantifying TTK mRNA in HCC samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
The mRNA expression of TTK in HCC specimens and their adjacent non-tumourous liver tissues were quantified using QuantiGene Plex 2.0® assay (Affymetrix) following the manufacturer's instructions. All the oligo nucleotide probe sets including capture, label and blocker probes were designed by the manufacturer. For each sample, 200 μg extracted total RNA was used at the beginning of the assay. Mean fluorescence intensity (MFI) generated from each specific probe set were captured and quantified using a Luminex instrument (Bio-Rad).
Liu X., Liao W., Yuan Q., Ou Y, & Huang J. (2015). TTK activates Akt and promotes proliferation and migration of hepatocellular carcinoma cells. Oncotarget, 6(33), 34309-34320.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!