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CycA[c03456] is a genetic stock of the fruit fly Drosophila melanogaster. It carries a mutation in the CycA gene, which is involved in cell cycle regulation. The core function of this stock is to provide researchers with a tool to study the role of the CycA gene in various biological processes.

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Lab products found in correlation

Bamgfp, by Bloomington Drosophila Stock Center (2 mentions) Cyca c8lr1, by Bloomington Drosophila Stock Center (2 mentions) Hsflp, by Bloomington Drosophila Stock Center (2 mentions) Cycb2, by Bloomington Drosophila Stock Center (2 mentions) 42dfrt gfp, by Bloomington Drosophila Stock Center (2 mentions) Cyca 03946, by Bloomington Drosophila Stock Center (2 mentions)

2 protocols using cyca c03456

1

Drosophila Germline Development Protocols

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Drosophila were grown on corn flour and agar media with brewer’s yeast. All strains were grown at 25 °C. pgcGFP, pgcΔ and pgcΔFRT fly lines were generated for this study. liprinγH1 and bamΔ86 flies were acquired from the Treisman Lab (Astigarraga et al., 2010 ) and McKearin Lab (McKearin and Ohlstein, 1995 (link)) respectively. nos-Gal4::VP16, UASCycB (Mathieu et al., 2013 (link)) and sco/CyO; MKRS/TM6 was acquired from the Lehmann lab. UASpCycT-nos3′UTR, UASpcdk9-nos3′UTR and Genomic pgc+ transgene was given to us by the Nakamura Lab (Hanyu-Nakamura et al., 2008 (link)). CycB2, bamGFP, hsFLP, 42DFRT-GFP, CycA[c03456], CycA[03946] and CycA[c8LR1] are available at Bloomington Drosophila Stock Center, Bloomington, IN (Wang and Lin, 2005 (link)). CycTRNAi (v103387) and cdk9RNAi (v103569) lines were acquired from VDRC stock center.
Flora P., Schowalter S., Wong-Deyrup S., DeGennaro M., Nasrallah M.A, & Rangan P. (2017). Transient transcriptional silencing alters the cell cycle to promote germline stem cell differentiation in Drosophila. Developmental biology, 434(1), 84-95.
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2

Drosophila Germline Development Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
Drosophila were grown on corn flour and agar media with brewer’s yeast. All strains were grown at 25 °C. pgcGFP, pgcΔ and pgcΔFRT fly lines were generated for this study. liprinγH1 and bamΔ86 flies were acquired from the Treisman Lab (Astigarraga et al., 2010 ) and McKearin Lab (McKearin and Ohlstein, 1995 (link)) respectively. nos-Gal4::VP16, UASCycB (Mathieu et al., 2013 (link)) and sco/CyO; MKRS/TM6 was acquired from the Lehmann lab. UASpCycT-nos3′UTR, UASpcdk9-nos3′UTR and Genomic pgc+ transgene was given to us by the Nakamura Lab (Hanyu-Nakamura et al., 2008 (link)). CycB2, bamGFP, hsFLP, 42DFRT-GFP, CycA[c03456], CycA[03946] and CycA[c8LR1] are available at Bloomington Drosophila Stock Center, Bloomington, IN (Wang and Lin, 2005 (link)). CycTRNAi (v103387) and cdk9RNAi (v103569) lines were acquired from VDRC stock center.
Flora P., Schowalter S., Wong-Deyrup S., DeGennaro M., Nasrallah M.A, & Rangan P. (2017). Transient transcriptional silencing alters the cell cycle to promote germline stem cell differentiation in Drosophila. Developmental biology, 434(1), 84-95.
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