For this experiment, 8–10 week old male C57BL/6 mice were anesthetized with Avertin (240 mg/kg intraperitoneally). An incision of 3 cm was performed through the center of the abdomen. Then the duodenum was exposed and the common bile duct was clamped near the liver. The intraductal injection was performed using a 28G1/2 U-100 Insulin Syringe into the pancreatic duct from the duodenum through the ampulla of Vater. The needle was clamped and 150 μl of saline or a single dose of a cocktail of proinflammatory cytokines ([1X] = TNFα [50ng/mL]; IL-1β [25ng/mL]; IFNγ [100ng/mL], n=3), ([2X] = TNFα [100ng/mL]; IL-1β [50ng/mL]; IFNγ [200ng/mL], n=6), ([10X] = TNFα [500ng/mL]; IL-1β [250ng/mL]; IFNγ [1000ng/mL], n=4) or saline injections (n=5) were administered. After clamp removal, the wound on the ampulla was sealed with 3M Vetbond Tissue Adhesive (3M Animal Care). Abdominal muscle layer was closed with interrupted suture and the overlying skin was sutured using Needle Dafilon® and Polyamide Monofilament (B. Braun). Blood glucose levels were measured once a week in the morning for 3 weeks. Pancreata were harvested 3 weeks post-injection.
Polyamide monofilament
Manufactured by B. Braun
Polyamide Monofilament is a type of lab equipment made of a single continuous thread of polyamide material. It is designed for use in various laboratory applications.
Automatically generated - may contain errors
3 protocols using polyamide monofilament
1
Cytokine-Induced Pancreatic Injury Model
2
Retrograde Pancreatic Duct Injection in Mice
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Mice were anesthetized with ketamine (100 mg/kg BW) and xylazine (10 mg/kg BW) and received a subcutaneous injection of buprenorphine (0.05 mg/kg BW). A midline incision of 2-to-3 cm was made along the abdomen. The duodenum was exposed and the common bile duct was clamped caudal to the liver. The intraductal injection was performed using a 28G1/2 needle. The syringe was entered into the pancreatic duct from the duodenum through the ampulla of Vater and carefully advanced in a retrograde manner. The needle was secured by a micro clamp and 100 μl of vehicle or capsaicin (50 μg/100 μl) were slowly administered over one minute. After clamp removal, the wound on the ampulla was sealed with 3M Vetbond Tissue Adhesive (3M Animal Care). Abdominal muscle layer was closed with simple interrupted stitch with Polyamide Monofilament (B. Braun) and the overlying skin was sutured using 9 mm autoclips (Braintree Scientific).
3
Surgical Exposure of Dorsal Root Ganglia
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Mice were anesthetized with ketamine (100 mg/kg BW) and xylazine (10 mg/kg BW) and received a subcutaneous injection of buprenorphine (0.05 mg/kg BW). The skin and muscles were separated exposing unilaterally the region of 11th, 12th and 13th thoracic vertebrae and the dorsal region transverse processes. To expose the DRGs, transverse processes were trimmed with a micro drill (Roboz Surgical Instrument). Whole DRGs were carefully cut with tipped Dumont #5 forceps and Vannas spring scissors (Fine Science Tools) without disrupting the spinal cord. Dorsal muscle layer was then closed with interrupted suture technique with Polyamide Monofilament (B. Braun). The overlying skin was sutured using 9 mm autoclips (Braintree Scientific).
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