Anti ndufa9

Manufactured by Merck Group

Anti-NDUFA9 is a laboratory reagent that targets the NDUFA9 protein, a subunit of the mitochondrial complex I. This product is used in research applications to study the structure, function, and regulation of complex I in cellular and biochemical systems.

Automatically generated - may contain errors

Lab products found in correlation

Criterion tgx stain free gel, by Bio-Rad (1 mentions) Donkey anti mouse hrp, by Thermo Fisher Scientific (1 mentions) Anti rcan1, by Merck Group (1 mentions) Anti sdha, by Merck Group (1 mentions) Anti opa1, by Merck Group (1 mentions) Anti β actin, by Merck Group (1 mentions) Gel doc ez imager, by Bio-Rad (1 mentions) Anti ndufs4, by Novus Biologicals (1 mentions) Anti gfap, by Merck Group (1 mentions) Anti cd68, by Abcam (1 mentions)

Close spelling variants of this product

NDUFA9

2 protocols using anti ndufa9

Western Blot Analysis of Mitochondrial Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

25 μg of islet or mitochondrial protein lysates were separated on a Criterion-TGX stain-free gel (Bio-Rad). Immunoreactive proteins were visualised on a Fuji-Film LAS4000 and Gel-Doc Ez-Imager (Bio-Rad). Primary antibodies; anti-RCAN1 (1:200, Sigma Aldrich), anti-TOM20 (1:1000, Jackson Laboratories), anti-β-actin (1:200, Sigma Aldrich), anti-NDUFA9 (1:1000, Sigma Aldrich), anti-SDHA (1:2000, Sigma Aldrich), anti-CORE1 (1:1200, Sigma Aldrich) and anti-Opa1 (1:1500, Sigma Aldrich). Secondary antibodies; donkey anti-rabbit horse-radish peroxidase (HRP) (1:100, Life Technologies) and donkey anti-mouse HRP (1:100, Life Technologies).

Peiris H., Duffield M.D., Fadista J., Jessup C.F., Kashmir V., Genders A.J., McGee S.L., Martin A.M., Saiedi M., Morton N., Carter R., Cousin M.A., Kokotos A.C., Oskolkov N., Volkov P., Hough T.A., Fisher E.M., Tybulewicz V.L., Busciglio J., Coskun P.E., Becker A., Belichenko P.V., Mobley W.C., Ryan M.T., Chan J.Y., Laybutt D.R., Coates P.T., Yang S., Ling C., Groop L., Pritchard M.A, & Keating D.J. (2016). A Syntenic Cross Species Aneuploidy Genetic Screen Links RCAN1 Expression to β-Cell Mitochondrial Dysfunction in Type 2 Diabetes. PLoS Genetics, 12(5), e1006033.

+ Open protocol

+ Expand

Antibody Panel for Mitochondrial Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies used included: anti-NDUFS4 (1:100 for immunohistochemistry and 1:1000 for western blot) was from Novus Biologicals (catalogue no. NBP1-31465); anti-CD68 (1:250) was from ABCAM; anti-GFAP (1:1000) was from Sigma; anti-NDUFA9, and anti- succinate dehydrogenase B (1:1000) were from MitoSciences (catalogue no. ab14713). Secondary antibodies were from Promega [catalogue nos. W4011 (rabbit) and W4021 (mouse)].

Corrà S., Cerutti R., Balmaceda V., Viscomi C, & Zeviani M. (2022). Double administration of self-complementary AAV9NDUFS4 prevents Leigh disease in Ndufs4−/− mice. Brain, 145(10), 3405-3414.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!