Oxaliplatin oxa

Human CRC cell lines HCT116 and SW480 were cultured in Dulbecco’s Modified Eagle Medium with 4.5 g/L glucose (DMEM; Gibco BRL, Grand Island, NY, USA) containing 10% fetal bovine serum (FBS; Gibco BRL) and 1% antibiotic/antimycotic solution. Cells were maintained at 37 °C in an atmosphere of 5% CO₂ and 95% room air.
LncRNA CRNDE siRNA, control siRNA, miR-181a-5p and miR-181a-5p inhibitor were all obtained from GenePharma (Shanghai, China). 5-fluorouracil (5-Fu) and oxaliplatin (Oxa) were all purchased from Sigma Aldrich (St Louis, MO, USA). Lipofectamine™ 3000 (Life Technologies, San Diego, CA, USA) was used for cell transfection according to manufacturer’s instructions. After 48 h of transfection, cells were treated different concentration of 5-Fu (0, 3, 6, 12, 24 and 48 μg/ml) or Oxa (0, 4, 8, 16, 32 and 64 μg/ml).

Iron (III) chloride hexahydrate (≥99%), iron (II) chloride tetrahydrate (98%), oleic acid (≥90%) (OA), potassium dihydrogen phosphate (≥98%), N-hydroxysuccinimide (NHS) (98%), N-(3-dimethyl aminoproyl)-N’-ethylcarbodiimide hydrochloride (EDC), N,N′-dicyclohexylcarbodiimide (DCC) (≥99.0%), poly(ethylene glycol diamine) (NH₂-PEG-NH₂, Mn = 3000), (≥98.0%), polyvinyl alcohol (PVA, Mowiol 4–88), deuterated chloroform (≥99%) and oxaliplatin (OXA) (≥99%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Dialysis bags (Spectra/Por, MWCO: 3.5 and 12–14 kDa) and a monoclonal anti-CD133-TMP4 Ab conjugated to Alexa Fluor 488 were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Commercial PLGA Mn of 10 kDa (Purasorb PDLG 7502A, 70/30 LA/GA) was kindly donated by Corbion (The Netherlands). Sodium hydroxide, chloroform, ethyl ether, tetrahydrofuran (THF), dichloromethane (DCM), ethanol, and ammonia p.a. were purchased from Penta (The Czech Republic). Media used for cell culture Eagle’s Minimum Essential Medium (EMEM) and Dulbecco’s Modified Eagles’ medium (DMEM) were bought from Thermo Fisher Scientific (Waltham, MA, USA). Microscopy dishes with glass bottom (ø35 mm, 1.5#) were supplied by MatTek Life Sciences (Boston, MA, USA). Filters with a 0.45-µm pore size were purchased from JetBiofil (China). For all experiments, Millipore water was used.