Following decapitation, trunk blood was collected immediately in BD Vacutainer EDTA collection tubes (BD, Franklin Lakes, NJ). Blood was spun down at 1800rcf and the plasma fraction was collected. Plasma corticosterone levels were assayed via ELISA: corticosterone (sensitivity: 27 pg/ml, Enzo Life Sciences, Farmingdale, NY, USA). Samples were run in duplicate.
Edta vacutainer collection tubes
Manufactured by BD
Sourced in United States
EDTA Vacutainer collection tubes are used for the collection and transportation of venous blood samples. These tubes contain the anticoagulant EDTA (Ethylenediaminetetraacetic acid) which prevents the blood from clotting during the collection process.
Automatically generated - may contain errors
Lab products found in correlation
Corticosterone, by Enzo Life Sciences (1 mentions)
Nucleospin virus kit, by Macherey-Nagel (1 mentions)
Brilliant 3 ultra fast qrt pcr master mix, by Agilent Technologies (1 mentions)
Stepone real time pcr system, by Thermo Fisher Scientific (1 mentions)
Qiaamp dna mini kit, by Qiagen (1 mentions)
Cfx96 real time pcr detection system, by Bio-Rad (1 mentions)
Histopaque 1077, by Merck Group (1 mentions)
70 μm cell strainer, by Corning (1 mentions)
Ack lysing buffer, by Quality Biological (1 mentions)
6 protocols using edta vacutainer collection tubes
1
Plasma Corticosterone Extraction Protocol
2
HIV RNA Viral Load and Resistance Assay
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Blood samples were collected in 8 ml BD Vacutainer EDTA collection tubes (BD, Franklin Lakes, New Jersey, USA). Cell-free plasma was collected by centrifugation at 956g for 5 min and frozen at −80°C until testing for HIV RNA viral load or viral drug resistance. Assays for viral load and sequencing for HIV drug resistance were performed at the Ifakara Health Institute laboratory in Ifakara. HIV RNA from 400 μl plasma was extracted using the NucleoSpin Virus kit (Macherey-Nagel, Oensingen, Switzerland) according to the manufacturer's protocol. Viral RNA quantification was performed using a validated in-house protocol [23 (link)] with the Brilliant III Ultra-Fast QRT-PCR Master Mix (Agilent Technologies, La Jolla, California, USA) using the StepOne Real-Time PCR System (Applied Biosystems, Foster City, California, USA), with a detection limit of 200 viral RNA copies/ml of plasma. HIV drug resistance genotyping was performed by Sanger sequencing on a 3130 Genetic Analyser 4-capillary model (Applied Biosystems, Foster City, California, USA) using a validated in-house PCR protocol [23 (link)].
3
Genotyping for COMT Haplotype in Burn Patients
4
Alloantibody Plasma Isolation from Volunteers
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All experiments using human blood cells were done following protocol approval by the University of British Columbia Clinical Research Ethics Board and in accordance with the Declaration of Helsinki. Following informed written consent, donor blood was collected in EDTA‐Vacutainer® collection tubes (BD, Franklin Lakes, NJ) from normal D+ and D− donors. RBC were washed (3×) in isotonic saline prior to use. De‐identified human anti‐D alloantibody plasma samples were obtained from volunteer blood donors at LifeShare Blood Centers (Shreveport, LA) and stored at −80°C until used. Peripheral blood mononuclear cells (PBMC) were isolated using histopaque‐1077 (Sigma‐Aldrich, St. Louis, MO) and washed (2×) with PBS prior to suspension in culture media.
5
Genetic Characterization of Hereditary Anemia in Malaysia
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This study was approved by the Universiti Kebangsaan Malaysia Ethics Committee and the ethics committee of the Ministry of Health of Malaysia. Written informed consent taken from all patients with confirmed non-familial HA (n=100) and HB (n=15) who were being followed-up at the National Blood Centre, Kuala Lumpur. Detailed clinical history along with pedigree data were taken, and the disease severity classification was as the following: 1) mild HA (FVIII/FIX:C:>5–40%), 2) moderate HA (FVIII/FIX:C:1–5%), and 3) severe HA (FVIII/FIX:C:<1%).21 (link) Venous blood (10mL) was collected in EDTA Vacutainer collection tubes (BD, New Jersey, USA) and proceeded to DNA extraction using the salting-out extraction method.22 (link) DNA quality and concentration were determined by using NanoDrop Spectrophotometry and gel electrophoresis according to the manufacturer’s instruction.
6
Isolation and Flow Cytometry of Immune Cells
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The spleen and inguinal lymph nodes were collected from each mouse 5 months after stereotaxic injection of α-syn PFF seeds or monomers. Single cell suspension was prepared by mincing and filtering through a 70-μm cell strainer (Corning). Splenic red blood cells were lysed by resuspension of cell pellet in 5 mL of ACK lysing buffer (QualityBiological) for 5 min. Cells were counted and 2 million cells were used for flow cytometry. Blood was collected from atrial puncture and immediately transferred into EDTA Vacutainer collection tubes (BD) and mixed. EDTA-treated blood was then transferred into 50-mL conical tubes and red blood cells were lysed with 5 mL of ACK lysing buffer (QualityBiological) for 5 min and quenched with 1× HBSS. Cells were counted and 2 million cells were used for flow cytometry.
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