Log In Sign up
The largest database of trusted experimental protocols

Anti ps317γh2ax 05636

Manufactured by Merck Group
Visit Supplier

Anti-pS317γH2AX (05636) is a laboratory research antibody that specifically recognizes the phosphorylated form of the histone variant H2AX at serine 317. This antibody can be used to detect and measure the levels of this post-translational modification, which is often associated with the cellular response to DNA damage.

Automatically generated - may contain errors

Lab products found in correlation

Histone h3 fl 136 and anti vinculin h 10, by Santa Cruz Biotechnology (2 mentions) Anti ps824kap 1 a300 767a, by Fortis Life Sciences (2 mentions) Anti ps15p53 ab1431 and anti polq ab80906, by Abcam (2 mentions) Anti brdu 555627, by BD (2 mentions) Parpi rucaparib ag 014699, by Selleck Chemicals (2 mentions) Abt 888, by Abbvie (2 mentions) Mitomycin c mmc, by Merck Group (2 mentions) Hydroxyurea hu, by Merck Group (2 mentions) Anti flag m2, by Merck Group (2 mentions)

2 protocols using anti ps317γh2ax 05636

1

Antibody-based DNA Damage Response

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies used in this study included: anti-PCNA (PC-10), anti-FANCD2 (FI-17), anti-RAD51 (H-92), anti-GST (B14), and Histone H3 (FL-136) and anti-vinculin (H-10) (Santa Cruz); anti-Flag (M2) (Sigma); anti-pS317CHK1 (2344), anti-pT68CHK2 (2661) (Cell signalling); anti-pS824KAP-1 (A300-767A) (Bethyl); anti-pS317γH2AX (05636) (Millipore); anti-pS15p53 (ab1431) and anti-POLQ (ab80906) (abcam); anti-BrdU (555627) (BD Pharmingen). Mitomycin C (MMC), cis-diamminedichloroplatinum(II) (Cisplatin, CDDP), and Hydroxyurea (HU) were purchased from Sigma. The PARPi rucaparib (AG-014699) was purchased from Selleckchem and ABT-888 from AbbVie. Rucaparib was used for all in vitro assays and ABT-888 was used for all in vivo experiments.
Ceccaldi R., Liu J.C., Amunugama R., Hajdu I., Primack B., Petalcorin M.I., O'Connor K.W., Konstantinopoulos P.A., Elledge S.J., Boulton S.J., Yusufzai T, & D'Andrea A.D. (2015). Homologous recombination-deficient tumors are hyper-dependent on POLQ-mediated repair. Nature, 518(7538), 258-262.
+ Open protocol
+ Expand
2

Antibody-based DNA Damage Response

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies used in this study included: anti-PCNA (PC-10), anti-FANCD2 (FI-17), anti-RAD51 (H-92), anti-GST (B14), and Histone H3 (FL-136) and anti-vinculin (H-10) (Santa Cruz); anti-Flag (M2) (Sigma); anti-pS317CHK1 (2344), anti-pT68CHK2 (2661) (Cell signalling); anti-pS824KAP-1 (A300-767A) (Bethyl); anti-pS317γH2AX (05636) (Millipore); anti-pS15p53 (ab1431) and anti-POLQ (ab80906) (abcam); anti-BrdU (555627) (BD Pharmingen). Mitomycin C (MMC), cis-diamminedichloroplatinum(II) (Cisplatin, CDDP), and Hydroxyurea (HU) were purchased from Sigma. The PARPi rucaparib (AG-014699) was purchased from Selleckchem and ABT-888 from AbbVie. Rucaparib was used for all in vitro assays and ABT-888 was used for all in vivo experiments.
Ceccaldi R., Liu J.C., Amunugama R., Hajdu I., Primack B., Petalcorin M.I., O'Connor K.W., Konstantinopoulos P.A., Elledge S.J., Boulton S.J., Yusufzai T, & D'Andrea A.D. (2015). Homologous recombination-deficient tumors are hyper-dependent on POLQ-mediated repair. Nature, 518(7538), 258-262.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!