Sodium acetate

TRIzol reagent (Thermo Fisher Scientific, cat# 15596018), TRIzol LS Reagent (Ambion, Cat# 10296010), chloroform (Sigma-Aldrich, Cat No. C2432), glycogen (Thermo Fisher Scientific, cat# R0551), 3 M sodium acetate (pH5.2, Quality Biological, cat# 351035721), ethyl alcohol (Thermo Scientific Richard-Allan Scientific, for in vitro diagnostic use), isopropyl alcohol (Thermo Scientific Richard-Allan Scientific, for in vitro diagnostic use), nuclease-free water (Cell Signaling Technology, cat# 12931S), mixture vortex (VWR, Analog Vortex Mixer), and centrifuge (Thermo Scientific, Legend Micro 21R). MagMAX Blood RNA Isolation Kit (ThermoFisher Scientific, Cat# AM1837), NucleoSpin for miRNA and RNA purification kit (Macherey-Nagel, Cat# 740971.50), and Plasma/Serum RNA Purification kit (Norgen Biotek Corp, Cat# 56100).

1E7-03 (purity above 98%) was synthesized by Enamine (Kiev, Ukraine) as previously described [3 (link)]. Acetonitrile and water containing 0.1% formic acid (FA) were Optima LC/MS grade (Fisher Scientific, Fair Lawn, NJ). High-purity nitrogen (99.9%) was purchased from Roberts Oxygen Co, Inc. (Rockville, MD). Other reagents were of analytical grade. Dimethyl sulphoxide (DMSO), acetone, hydrochloric acid and sodium hydroxide were from Fisher Scientific (Fair Lawn, NJ). Phosphate buffered saline (pH 7.4) was from Life Technologies (Grand Island, NY). Sodium acetate (pH 5.2) was from Quality Biological (Gaithersburg, MD).

1E7–03 (purity above 98%) was synthesized by Enamine (Kyiv, Ukraine) as previously described [14 (link)]. Dimethyl sulfoxide (DMSO), acetone, hydrochloric acid and sodium hydroxide were obtained from Fisher Scientific (Fair Lawn, NJ). Sodium acetate (pH 5.2) was from Quality Biological (Gaithersburg, MD). Phosphate buffered saline (pH 7.4) was from Life Technologies (Grand Island, NY).