Heat inactivated fetal bovine serum fbs hi

Dulbecco’s Modified Eagle Medium-High Glucose Medium (DMEM) and Heat Inactivated Fetal Bovine Serum (FBS-HI) were obtained from Atlanta Biologicals (Flowery Branch, GA). Penicillin/streptomycin and Hank’s Balanced Salt Solution (HBSS) were obtained from GIBCO (Waltham, MA). Dimethyl sulfoxide (DMSO), PGG, lipopolysaccharide from Salmonella enterica (LPS), interferon γ (IFN γ), and other general reagents and supplies were all purchased from Sigma-Aldrich Co. (St. Louis, MO) and VWR International (Radnor, PA).

Dulbecco’s Modified Eagle Medium-High Glucose Medium (DMEM) and Heat Inactivated Fetal Bovine Serum (FBS-HI) were obtained from Atlanta Biologicals (Flowery Branch, GA, USA). Penicillin/streptomycin and Hank’s Balanced Salt Solution (HBSS) were obtained from GIBCO (Waltham, MA, USA). Dimethyl sulfoxide (DMSO), 1,2,3,4,6-Penta-O-Galloyl-β-D-glucose (purity 96.8%), lipopolysaccharide from Salmonella enterica (LPS), interferon γ (IFNγ), and other general reagents were all purchased from Sigma-Aldrich Co. (St. Louis, MO, USA) and VWR International (Radnor, PA, USA). NFƙB and MAPK signaling pathways arrays, Bradford protein assay and PCR reagents were bought from Bio-Rad (Hercules, CA, USA). Protease and Phosphatase inhibitors were obtained from G-Biosciences (Saint Louis, MO, USA). Anti-goat IgG-horseradish peroxidase and anti-GAPDH antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA) and primary antibodies from Santa Cruz Biotechnology, Cell Signaling (Danvers, MA, USA), and Thermo Fisher Scientific (Waltham, MA, USA), as listed below.