Anti rna polymerase 2 antibody

Anti-HYPK and anti-β-actin antibody were obtained from Sigma. Anti-HSF1, anti-Hsp70 and anti-acteylated histone H4 antibody were purchased from Abcam. Anti-RNA polymerase II antibody was purchased from Imgenex. The anti-mouse and anti-rabbit secondary antibodies conjugated with horseradish peroxidase were purchased from Bangalore Genei (India). Cobalt chloride (CoCl₂) was purchased from Merck and MG132 was purchased from Calbiochem. Immobilon-P Transfer membrane was from Millipore; Taq polymerase was from Bioline and restriction enzymes were from New England Biolabs (NEB). Protease inhibitor mixture was purchased from Roche Applied Science. TRIzol reagent, Lipofectamine 2000 and Hygromycin were obtained from Invitrogen. Other molecular biology grade fine chemicals were procured locally.