Angpt 1

Manufactured by R&D Systems

Angpt-1 is a protein that is involved in the regulation of angiogenesis, the process of new blood vessel formation. It functions as a growth factor for endothelial cells, which are the cells that line the interior of blood vessels. Angpt-1 plays a role in the stabilization and maturation of blood vessels.

Automatically generated - may contain errors

Lab products found in correlation

Pakt ser473 d9e, by Cell Signaling Technology (1 mentions) Tnf α, by R&D Systems (1 mentions) Ubiquitin, by Cell Signaling Technology (1 mentions) Tie2 clone ab33, by Merck Group (1 mentions) Gata3 d13c9, by Cell Signaling Technology (1 mentions) Akt 11e7, by Cell Signaling Technology (1 mentions) β tubulin and gapdh, by Santa Cruz Biotechnology (1 mentions) Angpt 2, by R&D Systems (1 mentions) 96 well plate, by Corning (1 mentions) Egm 2, by Lonza (1 mentions) Ebm 2, by Lonza (1 mentions) Ripk3, by Cusabio (1 mentions) Dmem f12, by Thermo Fisher Scientific (1 mentions) Egm 2 singlequot, by Lonza (1 mentions) Gfp antibody, by Abcam (1 mentions) Ebm 2 media, by Lonza (1 mentions)

4 protocols using angpt 1

Endothelial Cell Signaling Pathway

Check if the same lab product or an alternative is used in the 5 most similar protocols

All chemicals and reagents were purchased from Sigma-Aldrich (St. Louis, MO) unless otherwise specified. Antibodies against Tie2 (C-20) (Santa Cruz Biotechnology, Inc.), Tie2 (clone Ab33) (Merck Millipore), GATA3 (D13C9) (Cell Signaling), MMP14/MT1-MMP (D1E4) (Cell Signaling), pAKT (Ser473, D9E) (Cell Signaling), AKT (11E7) (Cell Signaling) and ubiquitin (Cell Signaling) β-tubulin and GAPDH (Santa Cruz Biotechnology, Inc.) were used. Soluble Tie2 levels were determined using the human Tie-2 DuoSet ELISA and the mouse Tie-2 Quantikine Kit. HUVECs were stimulated with TNFα, Angpt-1 and Angpt-2 (all from R&D systems, Minneapolis, MN).

Thamm K., Schrimpf C., Retzlaff J., Idowu T.O., van Meurs M., Zijlstra J.G., Ghosh C.C., Zeitvogel J., Werfel T.A., Haller H., Parikh S.M, & David S. (2018). Molecular regulation of acute Tie2 suppression in sepsis. Critical care medicine, 46(9), e928-e936.

+ Open protocol

+ Expand

Endothelial Cell Response to COVID-19 Plasma

Check if the same lab product or an alternative is used in the 5 most similar protocols

HUVECs (pooled donor, Lonza) were grown and maintained in endothelial cell growth basal media (EBM-2, Lonza), containing 2% FBS and contents of the EGM-2 SingleQuots growth factor supplement kit. Cells from passages 3–5 were used for experiments. HUVECs were grown to confluency in 96-well plates (Corning) and incubated overnight with 10% pooled patient plasma and H-Gly-Pro-Arg-Pro-OH (GPRP, 5 mM, Cayman Chemical) in the presence of complete growth media. Supplementation of Angpt-1 (300 ng/mL, R&D Systems) or AKB-9778 (5 μM, a gift from Aerpio Pharmaceuticals, Inc.) was performed 30 minutes prior to addition of patient plasma. All endothelial cell–based studies were performed in a dedicated tissue culture room designated specifically for work with COVID-19 biospecimens.

Schmaier A.A., Pajares Hurtado G.M., Manickas-Hill Z.J., Sack K.D., Chen S.M., Bhambhani V., Quadir J., Nath A.K., Collier A.R., Ngo D., Barouch D.H., Shapiro N.I., Gerszten R.E., Yu X.G., Peters K.G., Flaumenhaft R, & Parikh S.M. (2021). Tie2 activation protects against prothrombotic endothelial dysfunction in COVID-19. JCI Insight, 6(20), e151527.

+ Open protocol

+ Expand

Measuring Plasma Protein Levels in ARDS

Check if the same lab product or an alternative is used in the 5 most similar protocols

Plasma samples from the ARDS and recovery cohorts were used for ELISAs, according to manufacturer recommendations. Human ANGPT2 (R&D Systems, Minneapolis, MN; catalog number DANG20) and RIPK3 (Cusabio, Houston, TX; catalog number CSB-EL019737HU) kits were used to measure plasma protein levels. In addition, CD40LG (R&D Systems; catalog number DCDL40) and ANGPT1 (R&D Systems; catalog number DANG10) were measured in high- and low-expressing patient samples (N = 6 each, 12 total) to validate the O-link platform. Plasma samples were diluted (1:8 dilution for ANGPT2, 1:10 dilution for RIPK3, 1:8 dilution for ANGPT1, and 1:15 dilution for CD40L) before plating. Final sample absorbance was measured at 450 nm with wavelength correction performed at 570 nm. Sample concentrations were calculated from a four-parameter logistic curve generated from known standard concentrations. Dilution factors were accounted for to calculate the final sample concentration. Plasma protein values were log10 transformed before statistical analysis.

Price D.R., Benedetti E., Hoffman K.L., Gomez-Escobar L., Alvarez-Mulett S., Capili A., Sarwath H., Parkhurst C.N., Lafond E., Weidman K., Ravishankar A., Cheong J.G., Batra R., Büyüközkan M., Chetnik K., Easthausen I., Schenck E.J., Racanelli A.C., Outtz Reed H., Laurence J., Josefowicz S.Z., Lief L., Choi M.E., Schmidt F., Borczuk A.C., Choi A.M., Krumsiek J, & Rafii S. (2022). Angiopoietin 2 Is Associated with Vascular Necroptosis Induction in Coronavirus Disease 2019 Acute Respiratory Distress Syndrome. The American Journal of Pathology, 192(7), 1001-1015.

+ Open protocol

+ Expand

Investigating TEK-CYP1B1 Interaction in HEK293 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

HEK293 cells were maintained in DMEM/F12 (Thermo Fisher) plus 10% FBS and penicillin/streptomycin. For TEK-CYP1B1 interaction studies, HEK293 cells were transiently transfected with plasmids encoding recombinant HA-TEK and GFP-tagged CYP1B1 proteins. Cells were then subjected to pull down using GFP antibody (Abcam, Cambridge, MA) crosslinked to AminoLink Plus Coupling Resin (Chromotek, Hauppauge, NY), as described earlier (Rao et al. 2016 (link)). The pulled-down proteins were washed three times with lysis buffer, and the samples were eluted in glycine lysis buffer (pH 2.8), neutralized using 1 M Tris pH 9.5, and analyzed by SDS-PAGE and immunoblotting.
HUVECs were maintained in EBM-2 media from Lonza (Walkersville, MD) supplemented with EGM-2 SingleQuots (Lonza). After transient transfection, the cells were treated with recombinant human Angiopoietin 1 (ANGPT-1; 600 ng/ml; R&D Systems, Minneapolis, MN), as described earlier (Souma et al. 2016 (link)). The cells were then fixed with 4% paraformaldehyde, blocked with 5% goat serum, and incubated with primary antibodies: HA (Abcam) and ZO-1 (Thermo Fisher) overnight. After washing, Alexa 488-conjugated and Alexa 546-conjugated secondary antibodies (Thermo Fisher) were added for 1 h. After washing, nuclear stain Hoechst was added, and the cells were imaged using a Leica microscope (DM5500).

Kabra M., Zhang W., Rathi S., Mandal A.K., Senthil S., Pyatla G., Ramappa M., Banerjee S., Shekhar K., Marmamula S., Mettla A.L., Kaur I., Khanna R.C., Khanna H, & Chakrabarti S. (2017). Angiopoietin receptor TEK interacts with CYP1B1 in primary congenital glaucoma. Human genetics, 136(8), 941-949.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!