Annexin 5 fitc

Hesperidin (purity >98%; determined by high-performance liquid chromatography) and MTT were obtained from Sigma-Aldrich; Merck KGaA (Darmstadt, Germany). Annexin V-FITC and propidium iodide were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China). Dulbecco's modified Eagle's medium (DMEM) and RPMI-1640 medium were purchased from HyClone (GE Healthcare, Chicago, IL, USA). Fetal bovine serum (FBS), penicillin and streptomycin were purchased from Tianjin HaoYang Biological Manufacture Co., Ltd. (Tianjin, China). Horseradish peroxidase-labeled anti-mouse and anti-rabbit secondary antibodies and all other antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). Cell culture plastic ware was purchased from BD Biosciences (San Jose, CA, USA).

Asiaticoside (purity >98%, determined by high-performance liquid chromatography), 3-(4, 5-dimethyl-2-thiazolyl) -2, 5-diphenyl-2H-tetrazolium bromide (MTT) were obtained from SigmaAldrich Chemical Co. (St. Louis, MO, USA). Annexin V-FITC and propidium iodide were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China). Dulbecco’s modified Eagle’s medium (DMEM) and RPMI-1640 medium were purchased from HyClone (Logan, UT, USA). Fetal bovine serum (FBS), penicillin, and streptomycin were purchased from Tianjin HaoYang Biological Manufacture Co., Ltd. (Tianjin, China). Horseradish peroxidase-labeled anti-mice and anti-rabbit secondary antibodies and all other antibodies were purchased from Cell Signaling Technology (MA, USA). Cell culture plasticware was purchased from BD Biosciences (San Jose, CA, USA).

Glychionide-A (purity >98%; determined by high-performance liquid chromatography), 3-(4, 5-dimethyl-2-thiazolyl)-2, and 5-diphenyl-2H-tetrazolium bromide (MTT) were obtained from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Annexin V-FITC and propidium iodide were purchased from Wuhan Boster Biological Technology (Wuhan, China). Dulbecco’s modified Eagle’s medium (DMEM) and RPMI-1640 medium were purchased from HyClone (Logan, UT, USA). Fetal bovine serum (FBS), penicillin, and streptomycin were purchased from Tianjin HaoYang Biological Manufacture Co. (Tianjin, China). Horseradish peroxidase-labeled anti-mouse and anti-rabbit secondary antibodies and all other antibodies were purchased from Cell Signalling Technology (MA, USA). Cell culture plasticware was purchased from BD Biosciences (San Jose, CA, USA).