The largest database of trusted experimental protocols

Percp conjugated cd3

Manufactured by BioLegend
Sourced in United States

PerCP-conjugated CD3 is a fluorescently-labeled antibody that binds to the CD3 antigen expressed on T cells. It is used for the identification and enumeration of T cells in flow cytometry applications.

Automatically generated - may contain errors

2 protocols using percp conjugated cd3

1

Phenotypic Analysis of Human PBMCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Peripheral blood mononuclear cells (PBMCs) were isolated from fresh blood by density gradient centrifugation on Ficoll-Paque PLUS (Cytiva). The isolated PBMCs were then analyzed with an 8-color flow cytometer (FACSCanto™II, BD Biosciences, CA, USA) after labeling with the following anti-human fluorochrome-conjugated antibodies: FITC-conjugated CD38 (clone HIT2); APC or FITC-conjugated PD-1 (clone EH12.2H7); FITC-conjugated CD45RA (clone HI100); PE-conjugated CD24 (clone ML5); PE-conjugated ICOS (clone C398.4A); PE-conjugated CCR6 (clone G034E3); PerCP-conjugated CD3 (clone UCHT1); PE/cyanine7-conjugated CD27 (clone O323); PE/cyanine7-conjugated CD4 (clone OKT4); APC-conjugated CD19 (clone HIB19); APC-conjugated CD45RO (clone UCHL1); APC-conjugated CXCR3 (clone G025H7); and Brilliant Violet 421-conjugated CXCR5 (clone J252D4) (all antibodies were from BioLegend, CA, USA). Cell viability was assessed using a live/dead assay (Fixable Viability Dye eFluor 780; eBioscience). All data were collected and analyzed using FlowJo software. The gating strategies of the isotype-matched IgG control for specific cell populations are shown in the supplementary figure (Figure S1).
+ Open protocol
+ Expand
2

Multiparametric Immunophenotyping of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fluorescent‐conjugated anti‐human antibodies including fluorescein isothiocyanate (FITC)‐conjugated CD16 (clone: B73.1), phycoerythrin (PE)‐conjugated CD56 (5.1H11), Peridinin‐chlorophyll‐protein complex (PerCP)‐conjugated NKp44 (clone: P44‐8), Allophycocyanin (APC)‐conjugated CD3 (clone: OKT3), FITC‐conjugated CD3 (clone: OKT3), PerCP‐conjugated CD3 (clone: HIT3a), PerCp‐Cy5 conjugated CXCR3 (clone: G025H7), PerCp‐Cy5‐conjugated NKG2D (clone: 1D11), APC‐conjugated CD11b (clone: ICRF44), PerCP‐Cy5.5–conjugated CD27 (clone: M‐T271), PerCP‐Cy5.5‐conjugated perforin (clone: B‐D48), Alexa Fluor® 647‐conjugated granzyme B (clone: GB11), PerCP‐Cy5.5‐conjugated CD96 (clone: NK92.39) and their cognate isotype controls (all from Biolegend) as well as PerCP‐Cy5.5‐conjugated NKG2C (clone: 134591, R&D system) antibodies were used for immunophenotyping. Lysing solution (BD Biosciences) was used for removing the red blood cells. For washing, fixing, and cell permeabilizing, 1X phosphate buffer saline (PBS), 1% paraformaldehyde solution (Sigma), and 1X perm/wash solution (BD Biosciences) were used, respectively.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!