After calcium imaging, DRG cells in the culture dish were fixed in formalin, followed by 30% sucrose, and then incubated with 5% normal serum. They were immunostained with rabbit anti-TRPV4 antibody (1:300; Abcam, Cambridge MA) at 4°C overnight, followed by incubation with the corresponding secondary antibody conjugated with Alexa Fluor 488 (1:500; Life Technologies, Grand Island, NY) for 2 h. DRG cells were counterstained with DAPI in the mounting medium (Vector Laboratories, Burlingame, CA). Images were captured using a fluorescence microscope (Nikon Eclipse Ti; Technical Instruments). Immunohistofluorescent images were aligned with images captured during calcium imaging to determine the percentages of 5-HT-responsive DRG cells that were double labeled for TRPV4.
Rabbit anti trpv4 antibody
Manufactured by Abcam
Rabbit anti-TRPV4 antibody is a primary antibody that specifically recognizes the transient receptor potential cation channel subfamily V member 4 (TRPV4) protein. TRPV4 is a calcium-permeable cation channel that plays a role in various cellular processes. This antibody can be used to detect and study the expression and localization of TRPV4 in biological samples.
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2 protocols using rabbit anti trpv4 antibody
1
Fluorescent Labeling of TRPV4 in DRG Cells
2
Fluorescent Labeling of TRPV4 in DRG Cells
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After calcium imaging, DRG cells in the culture dish were fixed in formalin, followed by 30% sucrose, and then incubated with 5% normal serum. They were immunostained with rabbit anti-TRPV4 antibody (1:300; Abcam, Cambridge MA) at 4°C overnight, followed by incubation with the corresponding secondary antibody conjugated with Alexa Fluor 488 (1:500; Life Technologies, Grand Island, NY) for 2 h. DRG cells were counterstained with DAPI in the mounting medium (Vector Laboratories, Burlingame, CA). Images were captured using a fluorescence microscope (Nikon Eclipse Ti; Technical Instruments). Immunohistofluorescent images were aligned with images captured during calcium imaging to determine the percentages of 5-HT-responsive DRG cells that were double labeled for TRPV4.
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