ELISA experiments were performed with fibroblast culture supernatant, TNF-α, and IL-1β ELISA kits, according to the manufacturer's instructions. The cell culture supernatant (100 μL) was pipetted into a 96-well plate to incubated for 2 hours, then washed 3 times with washing buffer, then the well plate was dried before 200 μL of substrate (tetramethylbenzopyridine) was added to every in the well. Let it react for 20 minutes. Plates were read by using an MCC 340 multi-scan plate reader (Thermo Fisher Scientific Inc, Pittsburgh, PA) at a wavelength of 450 nm. The expression of TNF-α and IL-1β in the samples were determined by comparison with a standard curve generated using a manufacturer-provided standard. The standard curve was completed by plotting average O.D. (450 nm) acquired for each concentration on the “Y” axis and average O.D. (450 nm) for the corresponding concentration on the horizontal (“X”) axis.
Mcc 340
Manufactured by Thermo Fisher Scientific
Sourced in United States
The MCC 340 is a laboratory instrument used for monitoring and controlling temperature-dependent processes. It features a digital display and supports a range of temperature sensors.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mcc 340
1
Quantifying Cytokine Expression in Fibroblasts
2
HUVEC Proliferation Viability Assay
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Cell proliferative viability of HUVECs was examined using CCK-8 commercial kit (Cat. No. C0038, Beyotime Biotech.) based on protocol of the manufacturer. Briefly, HUVECs were cultured to the dosage of 1×10 5 cells/ml and treasfected with microRNA-126 inhibitor and microRNA-126 control plasmids. Then, the transfected HUVECs were cultured for 24 h and administrated with CCK-8 regent for 4 h at 37 ℃ (at final dosage of 10 μl/ml).
At last, proliferative viabilities of HUVECs were evaluated using a micro-plate reader (Mode: MCC/340, Thermo Fisher Scientific, Hudson, NH, USA) at 450 nm.
At last, proliferative viabilities of HUVECs were evaluated using a micro-plate reader (Mode: MCC/340, Thermo Fisher Scientific, Hudson, NH, USA) at 450 nm.
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